Question 1

The gene for human insulin has been successfully cloned in

Accepted Answer

A)  S. aureus. 
B)  yeast. 
C) E. coli. 
D)  rhinovirus. 
A)  S. aureus. 
B)  yeast. 
C) E. coli. 
D)  rhinovirus.

Question 2

The polymerase chain reaction is used to duplicate small sections of

Accepted Answer

A)  DNA. 
B)  RNA. 
C)  proteins. 
D)  lipids. 
A)  DNA. 
B)  RNA. 
C)  proteins. 
D)  lipids.

Question 3

DNA molecules that contain pieces of DNA from two different sources are defined as

Accepted Answer

A)  biotechnology. 
B)  gene cloning. 
C)  recombinant DNA. 
D)  genetic engineering. 
A)  biotechnology. 
B)  gene cloning. 
C)  recombinant DNA. 
D)  genetic engineering.

Question 4

Knowing the sequence of a genome is useful in

Accepted Answer

A)  identifying genetic alterations associated with disease. 
B)  studying evolutionary relationships. 
C)  determining protein sequences. 
D)  All of the choices are correct. 
A)  identifying genetic alterations associated with disease. 
B)  studying evolutionary relationships. 
C)  determining protein sequences. 
D)  All of the choices are correct.

Question 5

In a FISH experiment, what would happen if unbound probe was not washed off?

Accepted Answer

A)  Nothing-it's not necessary to wash off the unbound probe. 
B)  You would get false positive results in different areas where the probe hadn't actually bound, but it was still sitting there and lighting up. 
C)  Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them. 
D)  Nothing-the target nucleotide sequences are labeled, not the probe. Therefore, excess unbound probe wouldn't matter for the experiment. 
A)  Nothing-it's not necessary to wash off the unbound probe. 
B)  You would get false positive results in different areas where the probe hadn't actually bound, but it was still sitting there and lighting up. 
C)  Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them. 
D)  Nothing-the target nucleotide sequences are labeled, not the probe. Therefore, excess unbound probe wouldn't matter for the experiment.

Question 6

A dye often used for its ease and sensitivity to visualize nucleic acid after agarose gel electrophoresis is

Accepted Answer

A)  nigrosin. 
B)  malachite green. 
C)  gold oxide. 
D)  ethidium bromide. 
A)  nigrosin. 
B)  malachite green. 
C)  gold oxide. 
D)  ethidium bromide.

Question 7

The Ti plasmid is used as a vector to transfer DNA into

Accepted Answer

A)  viruses. 
B)  bacteria. 
C)  plant cells. 
D)  animal cells. 
A)  viruses. 
B)  bacteria. 
C)  plant cells. 
D)  animal cells.

Question 8

In order to get around the lack of ability of prokaryotes to remove introns from precursor RNA, it may be necessary to

Accepted Answer

A)  use the DNA directly. 
B)  use the DNA after it has been processed. 
C)  use different promoters. 
D)  turn mRNA into cDNA. 
E)  use the DNA directly AND use the DNA after it has been processed. 
A)  use the DNA directly. 
B)  use the DNA after it has been processed. 
C)  use different promoters. 
D)  turn mRNA into cDNA. 
E)  use the DNA directly AND use the DNA after it has been processed.

Question 9

PCR typically results in the generation of fragments of all sizes.

Accepted Answer

A) True 
 B)False

Question 10

The energy to separate fragments during agarose gel electrophoresis is supplied by

Accepted Answer

A)  gravity. 
B)  active transport. 
C)  agarosis. 
D)  electricity. 
A)  gravity. 
B)  active transport. 
C)  agarosis. 
D)  electricity.

Question 11

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

Accepted Answer

A)  Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA. 
B)  No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer. 
C)  No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs. 
D)  Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process. 
A)  Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA. 
B)  No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer. 
C)  No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs. 
D)  Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process.

Question 12

Goal(s) of gene cloning may be to produce

Accepted Answer

A)  a protein. 
B)  many copies of the gene to be used as a probe. 
C)  many copies of the gene for sequencing. 
D)  more copies of the host cell. 
E)  a protein, many copies of the gene to be used as a probe AND many copies of the gene for sequencing. 
A)  a protein. 
B)  many copies of the gene to be used as a probe. 
C)  many copies of the gene for sequencing. 
D)  more copies of the host cell. 
E)  a protein, many copies of the gene to be used as a probe AND many copies of the gene for sequencing.

Question 13

PCR produces

Accepted Answer

A)  DNA fragments of all possible sizes. 
B)  DNA fragments that are one nucleotide larger than the next fragment. 
C)  DNA fragments of a particular size. 
D)  DNA fragments of increasing size. 
E)  DNA fragments that are one nucleotide larger than the next fragment AND DNA fragments of increasing size. 
A)  DNA fragments of all possible sizes. 
B)  DNA fragments that are one nucleotide larger than the next fragment. 
C)  DNA fragments of a particular size. 
D)  DNA fragments of increasing size. 
E)  DNA fragments that are one nucleotide larger than the next fragment AND DNA fragments of increasing size.

Question 14

Most eukaryotic genes are cloned directly into the vector for expression in prokaryotes.

Accepted Answer

A) True 
 B)False

Question 15

Host cells containing recombinant DNA can be selected on the basis of the properties of the

Accepted Answer

A)  vector. 
B)  ribosomes. 
C)  enzymes. 
D)  virus. 
A)  vector. 
B)  ribosomes. 
C)  enzymes. 
D)  virus.

Question 16

The molecules used as molecular scissors in genetic engineering is/are

Accepted Answer

A)  exonucleases. 
B)  proteases. 
C)  restriction enzymes. 
D)  RNA polymerase. 
A)  exonucleases. 
B)  proteases. 
C)  restriction enzymes. 
D)  RNA polymerase.

Question 17

DNA microarray technology

Accepted Answer

A)  may use many DNA fragments that function like probes. 
B)  attaches nucleotides to a solid support such as a glass slide. 
C)  relies on arrays that contain a detectable tag. 
D)  uses nucleic acid hybridization. 
E)  may use many DNA fragments that function like probes, attaches nucleotides to a solid support such as a glass slide AND uses nucleic acid hybridization. 
A)  may use many DNA fragments that function like probes. 
B)  attaches nucleotides to a solid support such as a glass slide. 
C)  relies on arrays that contain a detectable tag. 
D)  uses nucleic acid hybridization. 
E)  may use many DNA fragments that function like probes, attaches nucleotides to a solid support such as a glass slide AND uses nucleic acid hybridization.

Question 18

Taq polymerase is

Accepted Answer

A)  a reverse transcriptase. 
B)  an RNA polymerase. 
C)  from E. coli. 
D)  a heat stable DNA polymerase from Thermus aquaticus. 
E)  an RNA polymerase AND from E. coli. 
A)  a reverse transcriptase. 
B)  an RNA polymerase. 
C)  from E. coli. 
D)  a heat stable DNA polymerase from Thermus aquaticus. 
E)  an RNA polymerase AND from E. coli.

Question 19

A common vector used for cloning genes is/are

Accepted Answer

A)  bacteria. 
B)  viruses. 
C)  nucleotides. 
D)  plasmids. 
A)  bacteria. 
B)  viruses. 
C)  nucleotides. 
D)  plasmids.

Question 20

PCR is useful for amplifying a particular section of DNA.

Accepted Answer

A) True 
 B)False

Exam 9: Biotechnology and Recombinant Dna

The gene for human insulin has been successfully cloned in

The polymerase chain reaction is used to duplicate small sections of

DNA molecules that contain pieces of DNA from two different sources are defined as

Knowing the sequence of a genome is useful in

In a FISH experiment, what would happen if unbound probe was not washed off?

A dye often used for its ease and sensitivity to visualize nucleic acid after agarose gel electrophoresis is

The Ti plasmid is used as a vector to transfer DNA into

In order to get around the lack of ability of prokaryotes to remove introns from precursor RNA, it may be necessary to

PCR typically results in the generation of fragments of all sizes.

The energy to separate fragments during agarose gel electrophoresis is supplied by

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

Goal(s) of gene cloning may be to produce

PCR produces

Most eukaryotic genes are cloned directly into the vector for expression in prokaryotes.

Host cells containing recombinant DNA can be selected on the basis of the properties of the

The molecules used as molecular scissors in genetic engineering is/are

DNA microarray technology

Taq polymerase is

A common vector used for cloning genes is/are

PCR is useful for amplifying a particular section of DNA.

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Dynamics of Prokaryotic Growth

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From Dna to Protein

Bacterial Genetics

Identification and Classification of Prokaryotes

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Infections

Nervous System Infections

Blood and Lymphatic Infections

Hiv Disease and Complications of Immunodeficiency

Microbial Ecology

Environmental Microbiology: Treatment of Water, Wastes, and Polluted Habitats

Food Microbiology

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