Exam 5: The Genetics of Bacteria and Their Viruses

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A StrR mutant:

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A bacterium that is unable to grow on minimal medium is said to be:

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The following statements pertaining to Hfr, F+, F′, and/or F– strains of bacteria. Indicate which statements apply to each category.
Contains genes that regulate conjugation and the transfer of DNA from one cell to another.
Hfr
Always acts as the recipient in conjugation.
F+
F factor is integrated into the host chromosome.
F′
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Contains genes that regulate conjugation and the transfer of DNA from one cell to another.
Hfr
Always acts as the recipient in conjugation.
F+
F factor is integrated into the host chromosome.
F′
Cannot form a pilus.
F–
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Strain A of E.coli is auxotrophic for methionine,while strain B is auxotrophic for lysine.Both strains are F-.You want to produce a strain that is prototrophic.Design an experiment to produce the desired prototroph.Include in your experimental design your screening method for detecting the prototroph.

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