Exam 7: Enzyme Mechanisms

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A patient presents with symptoms associated with the disease beriberi.An analysis of pyruvate dehydrogenase complex activity shows significantly reduced levels from normal.Predict how a deficiency in a coenzyme may be the culprit.Specify the coenzyme involved.

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What three amino acids are targeted for phosphorylation by kinases?

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The mechanism of HMG-CoA reductase involves

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The Lineweaver-Burk plot shows data obtained for an enzyme in the absence and presence of a noncompetitive inhibitor.If the [I] is increased significantly in the experiment,the Vmaxapp would __________ and the Kmapp would __________. The Lineweaver-Burk plot shows data obtained for an enzyme in the absence and presence of a noncompetitive inhibitor.If the [I] is increased significantly in the experiment,the V<sub>maxapp</sub> would __________ and the K<sub>mapp</sub> would __________.

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In vivo arginine decarboxylase activity is monitored over several days in the absence and presence of protease inhibitors.The activity is greatly reduced when protease inhibitors are present.Arginine decarboxylase is not a protease.Interpret these findings.

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