Exam 16: Quality Assurance and Quality Control in the Molecular Laboratory

Which is the proper way to report a threonine-to-methionine mutation at amino acid position 790?

A blood sample is received in the laboratory for factor V Leiden analysis. What precautions are appropriate?

Which of the following organizations has established standards used by laboratory professionals when developing, implementing, and performing molecular-based laboratory tests?

What is the proper way to handle raw data from a completed test run?

A quantitative method was performed 100 times, and results from 99 of the 100 runs were in agreement with each other. This method has 99%:

What is required for incorporating an FDA-approved test into a laboratory?

An unlabeled bottle of clear liquid is found on a shelf. A sign on the shelf says, "Tris-EDTA Buffer, pH 8.0." What is the proper course of action?

A blood specimen is received in the laboratory for a PCR assay to detect cytomegalovirus (a DNA virus) on a Wednesday night. The PCR assay will not be performed until the next Monday morning. How should this specimen be stored to maintain the integrity of the nucleic acid prior to analysis?

A technologist in a tissue bank who performed HLA typing by DNA analysis was asked to bank all DNA extracted from specimens received in the laboratory so as to maintain the nucleic acid for the longest period in the most limited space. How should the DNA be stored to satisfy this request?

An outbreak of Salmonella has occurred in the community. Molecular-based assays will be performed to determine if multiple isolates of Salmonella taken at hospital X are related to each other and part of the outbreak. Laboratory technologists have accumulated 10 different cultures growing Salmonella and suspect that there may be more isolated in the next few days. How should these organisms be handled until the molecular-based assays can be performed in about 3 days?