Question 1

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Accepted Answer

A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  Class II antigens 
A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  Class II antigens

Question 2

Which of the following methods used to determine HLA types always gives high-resolution results?

Accepted Answer

A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT 
A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT

Question 3

The level of detail to which an allele is identified is called which of the following?

Accepted Answer

A)  Resolution 
B)  Ambiguity 
C)  Specificity 
D)  Typing 
A)  Resolution 
B)  Ambiguity 
C)  Specificity 
D)  Typing

Question 4

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

Accepted Answer

A)  HLA-D 
B)  mHag 
C)  KIR 
D)  HLA-A 
A)  HLA-D 
B)  mHag 
C)  KIR 
D)  HLA-A

Question 5

What are private epitopes?

Accepted Answer

A)  Molecules that bind to many different antibodies 
B)  Molecules only found on one individual 
C)  Molecules that bind to one specific HLA antibody type 
D)  Molecules that do not bind to any known HLA antibody 
A)  Molecules that bind to many different antibodies 
B)  Molecules only found on one individual 
C)  Molecules that bind to one specific HLA antibody type 
D)  Molecules that do not bind to any known HLA antibody

Question 6

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

Accepted Answer

A)  SBT 
B)  CDC 
C)  PCR-SSP 
D)  PCR-SSOP 
A)  SBT 
B)  CDC 
C)  PCR-SSP 
D)  PCR-SSOP

Question 7

DNA-based typing methods are used for which of the following?

Accepted Answer

A)  Determining serum antibody status 
B)  Crossmatching donors and recipients 
C)  Measuring T-cell-mediated cytotoxicity 
D)  Identifying HLA types 
A)  Determining serum antibody status 
B)  Crossmatching donors and recipients 
C)  Measuring T-cell-mediated cytotoxicity 
D)  Identifying HLA types

Question 8

HLA is an abbreviation for what term?

Accepted Answer

A)  Histocompatability locus array 
B)  Human leukocyte antibody 
C)  Human leukocyte antigen 
D)  Hepatic lipid antigen 
A)  Histocompatability locus array 
B)  Human leukocyte antibody 
C)  Human leukocyte antigen 
D)  Hepatic lipid antigen

Question 9

In PCR-SSOP, the HLA allele is identified based on which of the following?

Accepted Answer

A)  Primers that are specific for particular alleles 
B)  Probes that are specific for particular alleles 
C)  Restriction enzyme cutting and band patterns 
D)  Differences in single-strand conformations 
A)  Primers that are specific for particular alleles 
B)  Probes that are specific for particular alleles 
C)  Restriction enzyme cutting and band patterns 
D)  Differences in single-strand conformations

Question 10

A woman requires a kidney transplant. Five members of her family would like to be tested to determine if they are eligible donors. Technologists use sequence-specific oligonucleotide probe hybridization methods to perform HLA typing. Which of the following anticoagulants is preferred when collecting blood from the woman and her family members?

Accepted Answer

A)  None 
B)  Heparin 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium polyanethol sulfonate 
A)  None 
B)  Heparin 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium polyanethol sulfonate

Question 11

An HLA haplotype is inherited from:

Accepted Answer

A)  Only the mother 
B)  Only the father 
C)  Both the mother and the father 
D)  Neither the mother nor the father 
A)  Only the mother 
B)  Only the father 
C)  Both the mother and the father 
D)  Neither the mother nor the father

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Which of the following methods used to determine HLA types always gives high-resolution results?

The level of detail to which an allele is identified is called which of the following?

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

What are private epitopes?

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

DNA-based typing methods are used for which of the following?

HLA is an abbreviation for what term?

In PCR-SSOP, the HLA allele is identified based on which of the following?

An HLA haplotype is inherited from:

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 15: Dna-Based Tissue Typing

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Which of the following methods used to determine HLA types always gives high-resolution results?

The level of detail to which an allele is identified is called which of the following?

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

What are private epitopes?

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

DNA-based typing methods are used for which of the following?

HLA is an abbreviation for what term?

In PCR-SSOP, the HLA allele is identified based on which of the following?

An HLA haplotype is inherited from:

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

Filters