Question 1

Nucleic acids are injected into the capillary in capillary electrophoresis by which of the following injection methods?

Accepted Answer

A)  Hydrostatic 
B)  Electrokinetic 
C)  Pneumatic 
D)  Vacuum 
A)  Hydrostatic 
B)  Electrokinetic 
C)  Pneumatic 
D)  Vacuum

Question 2

Which of the following is not an example of pulse-field gel electrophoresis?

Accepted Answer

A)  Field inversion gel electrophoresis 
B)  Contour-clamped homogeneous electric field 
C)  Polyacrylamide gel electrophoresis 
D)  Transverse alternating field electrophoresis 
A)  Field inversion gel electrophoresis 
B)  Contour-clamped homogeneous electric field 
C)  Polyacrylamide gel electrophoresis 
D)  Transverse alternating field electrophoresis

Question 3

To increase the density of a sample relative to the density of the buffer prior to loading the sample into a gel, which of the following is added to the sample?

Accepted Answer

A)  Glycerol 
B)  Ethidium bromide 
C)  Formaldehyde 
D)  Tris acetate EDTA 
A)  Glycerol 
B)  Ethidium bromide 
C)  Formaldehyde 
D)  Tris acetate EDTA

Question 4

How does one prepare a 2% agarose gel?

Accepted Answer

A)  Mix 0.01 g agarose/mL running buffer and heat. 
B)  Mix 0.02 g agarose/mL running buffer and heat. 
C)  Mix 2 g agarose/mL running buffer and heat. 
D)  Mix 1 g agarose/mL running buffer and heat. 
A)  Mix 0.01 g agarose/mL running buffer and heat. 
B)  Mix 0.02 g agarose/mL running buffer and heat. 
C)  Mix 2 g agarose/mL running buffer and heat. 
D)  Mix 1 g agarose/mL running buffer and heat.

Question 5

To separate a mixture of oligonucleotides with a resolution between bands of 1 bp, which of the following methods should be used?

Accepted Answer

A)  Contour-clamped homogeneous electric field 
B)  Rotating gel electrophoresis 
C)  Polyacrylamide gel electrophoresis 
D)  Traditional agarose gel electrophoresis 
A)  Contour-clamped homogeneous electric field 
B)  Rotating gel electrophoresis 
C)  Polyacrylamide gel electrophoresis 
D)  Traditional agarose gel electrophoresis

Nucleic acids are injected into the capillary in capillary electrophoresis by which of the following injection methods?

Which of the following is not an example of pulse-field gel electrophoresis?

To increase the density of a sample relative to the density of the buffer prior to loading the sample into a gel, which of the following is added to the sample?

How does one prepare a 2% agarose gel?

To separate a mixture of oligonucleotides with a resolution between bands of 1 bp, which of the following methods should be used?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 5: Resolution and Detection of Nucleic Acids

Nucleic acids are injected into the capillary in capillary electrophoresis by which of the following injection methods?

Which of the following is not an example of pulse-field gel electrophoresis?

To increase the density of a sample relative to the density of the buffer prior to loading the sample into a gel, which of the following is added to the sample?

How does one prepare a 2% agarose gel?

To separate a mixture of oligonucleotides with a resolution between bands of 1 bp, which of the following methods should be used?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters