Exam 10: DNA Sequencing

In Maxam-Gilbert sequencing, a band that is present in the lane from the tube treated with formic acid (G or A) but not the tube treated with dimethyl sulfate (G) is read as which of the following nucleotides?

Dye terminator sequencing has been performed using capillary electrophoresis to separate the fragments. ddATP has been labeled with a "green" fluor, ddTTP with "red," ddCTP with "blue," and ddGTP with "black." The colors are detected in the following order: red, green, black, red, green, blue, red, black, black, green, blue. What is the sequence of this fragment?

Incomplete cleaning of the fluorescently labeled sequencing ladder will result in what effect?

The ddNTP:dNTP ratio is too high (too much ddNTP). How will this affect sequencing results?

Which is the output from capillary electrophoresis of a fluorescent product?

When two sequences are found to be exactly the same, they have which of the following?

In bioinformatics, when a sequence is entered and compared with all other sequences in a database, which of the following is being performed?

An analysis that takes place in silico is happening in which of the following places?

A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the fragments were resolved on the gel, there were bands distributed throughout the T, C and A lanes, but in the G lane, there was one thick band at the bottom of the gel. What is the likely cause of these results?

Which end of the sequenced DNA is found at the bottom of a sequencing gel?

Which of the following is the most widely used method of DNA sequencing in clinical laboratories today?

A portion of a sequencing gel is described as follows: the first band at the bottom of the gel was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as determined on this gel?

Dideoxycytidine is added to one of four sequencing reactions. All of the DNA fragments produced in that reaction end with which of the following nucleotides?

Which of the following is a genetic sequence database that is sponsored by the National Institutes of Health?

Sequencing ladders resolved by gel electrophoresis are read in which of the following directions?

Which of the following are included in sequencing reactions to deter secondary structure in the template and synthesized fragments?

Ten equivalents of a genome were cut into small pieces and sequenced. Computers were then used to put the sequences of the pieces together to determine the sequence of the intact genome. The method described is which of the following?

In the Sanger sequencing method, which of the following when incorporated into the growing strand are used to determine the identity of the base at a particular position in a piece of DNA?

Cycle sequencing was made possible through the development of what reagents?

A pyrosequencer detects visible light, making it which type of instrument?