Question 1

Which of the following is the first step in DNA isolation from cells in a clinical sample?

Accepted Answer

A)  DNA precipitation 
B)  Precipitation of proteins 
C)  Lysis of cells 
D)  Eluting DNA from a spin column 
A)  DNA precipitation 
B)  Precipitation of proteins 
C)  Lysis of cells 
D)  Eluting DNA from a spin column C

Question 2

Phenol/chloroform and an aqueous solution will form what type of mixture?

Accepted Answer

A)  Simple solution 
B)  Emulsion 
C)  Suspension 
D)  Supersaturated solution 
A)  Simple solution 
B)  Emulsion 
C)  Suspension 
D)  Supersaturated solution B

Question 3

What is the yield of DNA from a sample with concentration of 280 F1F1F1S1F1F1F10g/mL and a volume of 0.5 mL?

Accepted Answer

A)  70 F1F1F1S1F1F1F10g 
B)  140 F1F1F1S1F1F1F10g 
C)  280 F1F1F1S1F1F1F10g 
D)  560 F1F1F1S1F1F1F10g 
A)  70 F1F1F1S1F1F1F10g 
B)  140 F1F1F1S1F1F1F10g 
C)  280 F1F1F1S1F1F1F10g 
D)  560 F1F1F1S1F1F1F10g B

Question 4

The most abundant form of RNA in all cells is:

Accepted Answer

A)  Ribosomal 
B)  Transfer 
C)  Messenger 
D)  Small nuclear 
A)  Ribosomal 
B)  Transfer 
C)  Messenger 
D)  Small nuclear

Question 5

Which fixative is the least damaging to nucleic acids?

Accepted Answer

A)  B5 
B)  Bouin's 
C)  All mercury-based fixatives 
D)  Buffered formalin 
A)  B5 
B)  Bouin's 
C)  All mercury-based fixatives 
D)  Buffered formalin

Question 6

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

Accepted Answer

A)  DNA 
B)  RNA 
C)  Carbohydrate 
D)  Protein 
A)  DNA 
B)  RNA 
C)  Carbohydrate 
D)  Protein

Question 7

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

Accepted Answer

A)  6 F1F1F1S1F1F1F10g/mL 
B)  60 F1F1F1S1F1F1F10g/mL 
C)  75 F1F1F1S1F1F1F10g/mL 
D)  750 F1F1F1S1F1F1F10g/mL 
A)  6 F1F1F1S1F1F1F10g/mL 
B)  60 F1F1F1S1F1F1F10g/mL 
C)  75 F1F1F1S1F1F1F10g/mL 
D)  750 F1F1F1S1F1F1F10g/mL

Question 8

In what way is fluorometry more accurate than spectrophotometry?

Accepted Answer

A)  Fluorescence is more specific for single nucleotides. 
B)  Spectrophotometry only measures absorbance from intact nucleic acid polymers. 
C)  Using specific stains, fluorometry only detects intact double-stranded DNA. 
D)  Absorbance may require dilution of sample before reading. 
A)  Fluorescence is more specific for single nucleotides. 
B)  Spectrophotometry only measures absorbance from intact nucleic acid polymers. 
C)  Using specific stains, fluorometry only detects intact double-stranded DNA. 
D)  Absorbance may require dilution of sample before reading.

Question 9

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

Accepted Answer

A)  Electrophoresis 
B)  Enzyme immunoassay 
C)  Sequencing 
D)  Spectrophotometry at 260 nm 
A)  Electrophoresis 
B)  Enzyme immunoassay 
C)  Sequencing 
D)  Spectrophotometry at 260 nm

Question 10

DNA, after separation from other cellular constituents, is precipitated in which of the following?

Accepted Answer

A)  Chloroform 
B)  Isopropanol 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium dodecyl sulfate 
A)  Chloroform 
B)  Isopropanol 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium dodecyl sulfate

Question 11

Which whole blood fraction is the most abundant source of genomic DNA?

Accepted Answer

A)  Red blood cells 
B)  Buffy coat 
C)  Serum 
D)  Plasma 
A)  Red blood cells 
B)  Buffy coat 
C)  Serum 
D)  Plasma

Question 12

A DNA-specific dye that is used in fluorometry procedures to measure DNA concentration is:

Accepted Answer

A)  Ethidium bromide 
B)  Diethyl pyrocarbonate 
C)  SYBR Green 
D)  Hoechst 33258 
A)  Ethidium bromide 
B)  Diethyl pyrocarbonate 
C)  SYBR Green 
D)  Hoechst 33258

Question 13

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

Accepted Answer

A)  Carrier molecules 
B)  Detergent 
C)  Tris buffer 
D)  Isotonic saline 
A)  Carrier molecules 
B)  Detergent 
C)  Tris buffer 
D)  Isotonic saline

Question 14

Which of the following will yield the best DNA preparation?

Accepted Answer

A)  Fixed tissue 
B)  Hemolyzed blood 
C)  Fresh blood 
D)  Stool 
A)  Fixed tissue 
B)  Hemolyzed blood 
C)  Fresh blood 
D)  Stool

Question 15

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

Accepted Answer

A)  33.75 F1F1F1S1F1F1F10g/mL 
B)  337.5 F1F1F1S1F1F1F10g/mL 
C)  27 F1F1F1S1F1F1F10g/mL 
D)  270 F1F1F1S1F1F1F10g/mL 
A)  33.75 F1F1F1S1F1F1F10g/mL 
B)  337.5 F1F1F1S1F1F1F10g/mL 
C)  27 F1F1F1S1F1F1F10g/mL 
D)  270 F1F1F1S1F1F1F10g/mL

Question 16

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

Accepted Answer

A)  Yes 
B)  No 
C)  Cannot be determined with the data given 
A)  Yes 
B)  No 
C)  Cannot be determined with the data given

Question 17

In organic DNA extraction methods, the DNA is found in which fraction?

Accepted Answer

A)  Upper aqueous 
B)  Lower aqueous 
C)  Upper hydrophobic 
D)  Lower hydrophobic 
A)  Upper aqueous 
B)  Lower aqueous 
C)  Upper hydrophobic 
D)  Lower hydrophobic

Question 18

PolyT oligomers bound to a matrix resin column will selectively isolate which of the following?

Accepted Answer

A)  Ribosomal RNA 
B)  GC-rich DNA 
C)  Transfer RNA 
D)  Messenger RNA 
A)  Ribosomal RNA 
B)  GC-rich DNA 
C)  Transfer RNA 
D)  Messenger RNA

Question 19

Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191. What is the 260 nm/280 nm ratio?

Accepted Answer

A)  1:2 
B)  1:4 
C)  1:6 
D)  1:8 
A)  1:2 
B)  1:4 
C)  1:6 
D)  1:8

Question 20

What is the yield of RNA from the sample with concentration of 450 F1F1F1S1F1F1F10g/mL and a volume of 0.3 mL?

Accepted Answer

A)  135 F1F1F1S1F1F1F10g 
B)  150 F1F1F1S1F1F1F10g 
C)  225 F1F1F1S1F1F1F10g 
D)  450 F1F1F1S1F1F1F10g 
A)  135 F1F1F1S1F1F1F10g 
B)  150 F1F1F1S1F1F1F10g 
C)  225 F1F1F1S1F1F1F10g 
D)  450 F1F1F1S1F1F1F10g

Which of the following is the first step in DNA isolation from cells in a clinical sample?

Phenol/chloroform and an aqueous solution will form what type of mixture?

What is the yield of DNA from a sample with concentration of 280 g/mL and a volume of 0.5 mL?

The most abundant form of RNA in all cells is:

Which fixative is the least damaging to nucleic acids?

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

In what way is fluorometry more accurate than spectrophotometry?

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

DNA, after separation from other cellular constituents, is precipitated in which of the following?

Which whole blood fraction is the most abundant source of genomic DNA?

A DNA-specific dye that is used in fluorometry procedures to measure DNA concentration is:

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

Which of the following will yield the best DNA preparation?

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

In organic DNA extraction methods, the DNA is found in which fraction?

PolyT oligomers bound to a matrix resin column will selectively isolate which of the following?

Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191. What is the 260 nm/280 nm ratio?

What is the yield of RNA from the sample with concentration of 450 g/mL and a volume of 0.3 mL?

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 4: Nucleic Acid Extraction Methods

Which of the following is the first step in DNA isolation from cells in a clinical sample?

Phenol/chloroform and an aqueous solution will form what type of mixture?

What is the yield of DNA from a sample with concentration of 280 g/mL and a volume of 0.5 mL?

The most abundant form of RNA in all cells is:

Which fixative is the least damaging to nucleic acids?

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

In what way is fluorometry more accurate than spectrophotometry?

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

DNA, after separation from other cellular constituents, is precipitated in which of the following?

Which whole blood fraction is the most abundant source of genomic DNA?

A DNA-specific dye that is used in fluorometry procedures to measure DNA concentration is:

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

Which of the following will yield the best DNA preparation?

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

In organic DNA extraction methods, the DNA is found in which fraction?

PolyT oligomers bound to a matrix resin column will selectively isolate which of the following?

Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191. What is the 260 nm/280 nm ratio?

What is the yield of RNA from the sample with concentration of 450 g/mL and a volume of 0.3 mL?

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters