Question 1

What is learned from the size of restriction fragments as determined by migration speed in gel electrophoresis?

Accepted Answer

A)  The specificity of the restriction enzyme 
B)  The distance between restriction enzyme recognition sites 
C)  The rate of digestion of the enzyme 
D)  The number of restriction sites in the DNA 
A)  The specificity of the restriction enzyme 
B)  The distance between restriction enzyme recognition sites 
C)  The rate of digestion of the enzyme 
D)  The number of restriction sites in the DNA B

Question 2

A circular molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4 C

Question 3

What is the next step in the Southern blot procedure after restriction enzyme digestion?

Accepted Answer

A)  Denaturation 
B)  Gel electrophoresis 
C)  Blotting to a membrane 
D)  Probe hybridization 
A)  Denaturation 
B)  Gel electrophoresis 
C)  Blotting to a membrane 
D)  Probe hybridization B

Question 4

A northern blot allows you to do which of the following?

Accepted Answer

A)  Analyze a specific region of DNA in a complex background 
B)  Measure gene expression 
C)  Investigate DNA binding proteins 
D)  Determine antigen/antibody interactions 
A)  Analyze a specific region of DNA in a complex background 
B)  Measure gene expression 
C)  Investigate DNA binding proteins 
D)  Determine antigen/antibody interactions

Question 5

The entire human genome can be analyzed at one time by which of the following methods?

Accepted Answer

A)  Northern blot 
B)  Southern blot 
C)  Slot blot 
D)  Microarray 
A)  Northern blot 
B)  Southern blot 
C)  Slot blot 
D)  Microarray

Question 6

Which of the following is not a method of transferring nucleic acids from a gel to a membrane substrate?

Accepted Answer

A)  Capillary 
B)  Vacuum 
C)  Electrophoretic 
D)  Ionic 
A)  Capillary 
B)  Vacuum 
C)  Electrophoretic 
D)  Ionic

Question 7

A linear molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 8

Which of the following conditions are the least stringent?

Accepted Answer

A)  40% formamide, 1X SSC (salt), 50oC 
B)  50% formamide, 1X SSC, 55oC 
C)  50% formamide, 0.5X SSC, 55oC 
D)  40% formamide, 0.2X SSC, 60oC 
A)  40% formamide, 1X SSC (salt), 50oC 
B)  50% formamide, 1X SSC, 55oC 
C)  50% formamide, 0.5X SSC, 55oC 
D)  40% formamide, 0.2X SSC, 60oC

Question 9

Which of the following solutions has the highest stringency?

Accepted Answer

A)  0% formamide 
B)  10% formamide 
C)  high-salt buffer 
D)  50% formamide 
A)  0% formamide 
B)  10% formamide 
C)  high-salt buffer 
D)  50% formamide

Question 10

Which of the following methods is best suited for qualitative analysis of DNA, such as mutational analysis, where many samples are compared simultaneously?

Accepted Answer

A)  Dot blot 
B)  Traditional Southern blot 
C)  Western blot 
D)  Northern blot 
A)  Dot blot 
B)  Traditional Southern blot 
C)  Western blot 
D)  Northern blot

Question 11

The melting temperature of nucleic acid is the temperature when:

Accepted Answer

A)  All of the double-stranded DNA is single-stranded 
B)  Half of the double-stranded DNA is single-stranded 
C)  A quarter of the double-stranded DNA is single-stranded 
D)  All of the single-stranded DNA is double-stranded 
A)  All of the double-stranded DNA is single-stranded 
B)  Half of the double-stranded DNA is single-stranded 
C)  A quarter of the double-stranded DNA is single-stranded 
D)  All of the single-stranded DNA is double-stranded

Question 12

A circular molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 13

Which of the following molecules comprises most membranes that bind to nucleic acids in blotting procedures?

Accepted Answer

A)  Nitrocellulose 
B)  Agarose 
C)  Acrylamide 
D)  Formamide 
A)  Nitrocellulose 
B)  Agarose 
C)  Acrylamide 
D)  Formamide

Question 14

What was the great advantage to molecular analysis offered by Southern blot?

Accepted Answer

A)  Ability to analyze specific regions of DNA without cloning them 
B)  A rapid method to eliminate DNA contaminants 
C)  A more efficient buffer system 
D)  Ability to analyze nanogram amounts of DNA 
A)  Ability to analyze specific regions of DNA without cloning them 
B)  A rapid method to eliminate DNA contaminants 
C)  A more efficient buffer system 
D)  Ability to analyze nanogram amounts of DNA

Question 15

A linear molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 16

How do gene expression arrays differ from comparative genome hybridization (CGH) arrays?

Accepted Answer

A)  CGH arrays detect gain or loss of DNA regions; gene expression arrays detect changes in gene expression. 
B)  CGH arrays can interrogate hundreds of genes, while gene expression arrays test thousands. 
C)  CGH arrays are simpler to perform than gene expression arrays. 
D)  CGH arrays require co-hybridization with comparative reference nucleic acid, while gene expression arrays do not. 
A)  CGH arrays detect gain or loss of DNA regions; gene expression arrays detect changes in gene expression. 
B)  CGH arrays can interrogate hundreds of genes, while gene expression arrays test thousands. 
C)  CGH arrays are simpler to perform than gene expression arrays. 
D)  CGH arrays require co-hybridization with comparative reference nucleic acid, while gene expression arrays do not.

Question 17

What process facilitates denaturation of long DNA fragments?

Accepted Answer

A)  Precipitation 
B)  Removal of bases from the ends of the molecules 
C)  Dephosphorylation 
D)  Depurination 
A)  Precipitation 
B)  Removal of bases from the ends of the molecules 
C)  Dephosphorylation 
D)  Depurination

Question 18

What determines the specificity in terms of the gene or region that is detected in the Southern blot?

Accepted Answer

A)  Nitrocellulose membranes 
B)  Agarose gel 
C)  Restriction enzymes 
D)  Labeled probe 
A)  Nitrocellulose membranes 
B)  Agarose gel 
C)  Restriction enzymes 
D)  Labeled probe

Question 19

Which of the following bind(s) specifically to a sequence of interest, thus facilitating the analysis of that sequence?

Accepted Answer

A)  Restriction enzymes 
B)  Probes 
C)  Hybridomas 
D)  Digoxigenin 
A)  Restriction enzymes 
B)  Probes 
C)  Hybridomas 
D)  Digoxigenin

Question 20

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

Accepted Answer

A)  Horseradish peroxidase with Luminol/H2O2/p-iodophenol 
B)  Alkaline phosphatase with 1-2 dioxetane derivatives 
C)  Horseradish peroxidase with disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2F1F1F1S1F1F1F10-(5F1F1F1S1F1F1F10-chloro) tricyclo [3.3.1.1] decan}4-yl)-1-phenyl phosphate 
D)  Alkaline phosphatase with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate 
A)  Horseradish peroxidase with Luminol/H2O2/p-iodophenol 
B)  Alkaline phosphatase with 1-2 dioxetane derivatives 
C)  Horseradish peroxidase with disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2F1F1F1S1F1F1F10-(5F1F1F1S1F1F1F10-chloro) tricyclo [3.3.1.1] decan}4-yl)-1-phenyl phosphate 
D)  Alkaline phosphatase with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate

What is learned from the size of restriction fragments as determined by migration speed in gel electrophoresis?

A circular molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

What is the next step in the Southern blot procedure after restriction enzyme digestion?

A northern blot allows you to do which of the following?

The entire human genome can be analyzed at one time by which of the following methods?

Which of the following is not a method of transferring nucleic acids from a gel to a membrane substrate?

A linear molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Which of the following conditions are the least stringent?

Which of the following solutions has the highest stringency?

Which of the following methods is best suited for qualitative analysis of DNA, such as mutational analysis, where many samples are compared simultaneously?

The melting temperature of nucleic acid is the temperature when:

A circular molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Which of the following molecules comprises most membranes that bind to nucleic acids in blotting procedures?

What was the great advantage to molecular analysis offered by Southern blot?

A linear molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

How do gene expression arrays differ from comparative genome hybridization (CGH) arrays?

What process facilitates denaturation of long DNA fragments?

What determines the specificity in terms of the gene or region that is detected in the Southern blot?

Which of the following bind(s) specifically to a sequence of interest, thus facilitating the analysis of that sequence?

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 6: Analysis and Characterization of Nucleic Acids and Proteins

What is learned from the size of restriction fragments as determined by migration speed in gel electrophoresis?

A circular molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

What is the next step in the Southern blot procedure after restriction enzyme digestion?

A northern blot allows you to do which of the following?

The entire human genome can be analyzed at one time by which of the following methods?

Which of the following is not a method of transferring nucleic acids from a gel to a membrane substrate?

A linear molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Which of the following conditions are the least stringent?

Which of the following solutions has the highest stringency?

Which of the following methods is best suited for qualitative analysis of DNA, such as mutational analysis, where many samples are compared simultaneously?

The melting temperature of nucleic acid is the temperature when:

A circular molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

Which of the following molecules comprises most membranes that bind to nucleic acids in blotting procedures?

What was the great advantage to molecular analysis offered by Southern blot?

A linear molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

How do gene expression arrays differ from comparative genome hybridization (CGH) arrays?

What process facilitates denaturation of long DNA fragments?

What determines the specificity in terms of the gene or region that is detected in the Southern blot?

Which of the following bind(s) specifically to a sequence of interest, thus facilitating the analysis of that sequence?

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters