Exam 12: Detection and Identification of Microorganisms

A positive control is added (spiked) into a test specimen. The test results of the specimen alone and the spiked mixture are negative. What does this most likely indicate?

DNA is isolated from a test organism and cut with restriction enzymes. The fragments are ligated to adapters that are complementary to primers that are added to perform two rounds of PCR. The PCR products are separated by gel electrophoresis and analyzed. Which of the following methods was described?

The following methods that are used to determine the HIV viral load in a newly diagnosed patient. Which uses signal amplification?

In the interpretation of pulsed field gel electrophoresis patterns, what is the minimum number of bands that can differ between two organisms before those organisms are determined to be unrelated?

Which is a flow cytometry test?

What characteristic of parasites complicates molecular testing?

A PCR assay that uses short primers that amplify random sequences found throughout the genome of the test organism is what variation of PCR?

In a PCR assay of DNA isolated from a nasopharyngeal swab, amplification using primers to IS481 was positive and using primers to IS1001 was negative. All controls were acceptable. Which of the following organisms was present in this sample?

What will be observed in a PCR test of a true negative sample for the sample, positive control, negative control, and amplification control, respectively?

Which of the following is an important characteristic of a target sequence for molecular detection of a microorganism?

When a typing method is repeated and the results are the same each time, the method has high:

Which of the following typing methods is an RNA test?

Pulsed field gel electrophoresis has been performed on two isolates of Escherichia coli from the blood of newborns in the same neonatal unit. The banding pattern of one isolate had two differences from the banding pattern of the other isolate. Which of the following is the correct interpretation of these results?

Which of the following genes is the target in molecular-based assays that are used to quantify the amount of HIV in a patient?

Which of the following molecular-based assays targets rRNA?

Which virus does not share sequence homology with the others?

Which of the following targets are used to type molds?

HIV genotyping is performed for which of the following reasons?

What is a convenient way to concentrate organisms present in cerebrospinal fluid or urine samples before testing?

The ability of a typing method to distinguish between unrelated strains is which of the following criteria?