Question 1

What reactions are catalyzed by DNA methyltransferase?

Accepted Answer

A)  Addition of methyl groups to nitrogen bases 
B)  Addition of methyl groups to ribose sugars 
C)  Addition of methyl groups to proteins 
D)  Removal of methyl groups from DNA 
A)  Addition of methyl groups to nitrogen bases 
B)  Addition of methyl groups to ribose sugars 
C)  Addition of methyl groups to proteins 
D)  Removal of methyl groups from DNA A

Question 2

Nucleotides in DNA consist of which of the following?

Accepted Answer

A)  Nitrogen base, deoxyribose, and phosphate 
B)  Nitrogen base, ribose, and sulfur 
C)  Carbon base, ribose, and phosphate 
D)  Carbon base, glucose, and carboxyl 
A)  Nitrogen base, deoxyribose, and phosphate 
B)  Nitrogen base, ribose, and sulfur 
C)  Carbon base, ribose, and phosphate 
D)  Carbon base, glucose, and carboxyl A

Question 3

The purpose of ligase is to:

Accepted Answer

A)  Synthesize DNA 
B)  Cut DNA within the double helix 
C)  Chew DNA from the ends 
D)  Paste two ends of DNA together 
A)  Synthesize DNA 
B)  Cut DNA within the double helix 
C)  Chew DNA from the ends 
D)  Paste two ends of DNA together D

Question 4

The movement of DNA from one bacterium to another through the activity of bacteriophages is called:

Accepted Answer

A)  Conjugation 
B)  Transformation 
C)  Transduction 
D)  Crossing over 
A)  Conjugation 
B)  Transformation 
C)  Transduction 
D)  Crossing over

Question 5

DNA replication proceeds with the new daughter strand synthesized in the following orientation:

Accepted Answer

A)  5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10to 5F1F1F1S1F1F1F10 
C)  The daughter strand is not synthesized in any order. 
D)  Either 5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10or 3F1F1F1S1F1F1F10 to 5F1F1F1S1F1F1F10 
A)  5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10to 5F1F1F1S1F1F1F10 
C)  The daughter strand is not synthesized in any order. 
D)  Either 5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10or 3F1F1F1S1F1F1F10 to 5F1F1F1S1F1F1F10

Question 6

What is the fertility factor (F) observed by Hayes and Lederberg?

Accepted Answer

A)  A chemical that encourages bacterial mating 
B)  A virus 
C)  A plasmid 
D)  A polysaccharide on the cell surface 
A)  A chemical that encourages bacterial mating 
B)  A virus 
C)  A plasmid 
D)  A polysaccharide on the cell surface

Question 7

Which of the five ribose carbons of the deoxyribonucleotide are involved in formation of the DNA chain?

Accepted Answer

A)  1' and 5' 
B)  2' and 4' 
C)  3' and 4' 
D)  3' and 5' 
A)  1' and 5' 
B)  2' and 4' 
C)  3' and 4' 
D)  3' and 5'

Question 8

An exonuclease will catalyze what type of reaction?

Accepted Answer

A)  Polymerization of nucleotides into long strands 
B)  Separation of complementary DNA strands 
C)  Dissociation of phosphodiester bonds at the ends of DNA molecules 
D)  Formation of circular DNA molecules from linear ones 
A)  Polymerization of nucleotides into long strands 
B)  Separation of complementary DNA strands 
C)  Dissociation of phosphodiester bonds at the ends of DNA molecules 
D)  Formation of circular DNA molecules from linear ones

Question 9

Enzymes that recognize palindromic sequences of DNA, that are cut within the recognition sequence, that do not have methylating activity, and that are used frequently in the laboratory are which type of restriction enzymes?

Accepted Answer

A)  Type I 
B)  Type II 
C)  Type III 
D)  Type IV 
A)  Type I 
B)  Type II 
C)  Type III 
D)  Type IV

Question 10

In a double-stranded DNA molecule, base pairing between strands occurs between:

Accepted Answer

A)  A purine and a purine 
B)  A pyrimidine and a pyrimidine 
C)  A purine and a pyrimidine 
D)  Any type of nucleotide base 
A)  A purine and a purine 
B)  A pyrimidine and a pyrimidine 
C)  A purine and a pyrimidine 
D)  Any type of nucleotide base

Question 11

DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond?

Accepted Answer

A)  Aldehyde 
B)  Ester 
C)  Phosphodiester 
D)  Peptide 
A)  Aldehyde 
B)  Ester 
C)  Phosphodiester 
D)  Peptide

Question 12

The process of separating the two DNA strands into two single strands is called:

Accepted Answer

A)  Denaturation 
B)  Bidirectional 
C)  Depolymerization 
D)  Synthesis 
A)  Denaturation 
B)  Bidirectional 
C)  Depolymerization 
D)  Synthesis

Question 13

In DNA replication, the leading strand is the strand that has which orientation?

Accepted Answer

A)  5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10to 5F1F1F1S1F1F1F10 
C)  Both strands are leading. 
A)  5F1F1F1S1F1F1F10to 3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10to 5F1F1F1S1F1F1F10 
C)  Both strands are leading.

Question 14

In a DNA molecule, one end has a free hydroxyl group and one end has a free ___________.

Accepted Answer

A)  phosphate group 
B)  hydroxyl group 
C)  amino group 
D)  carboxyl group 
A)  phosphate group 
B)  hydroxyl group 
C)  amino group 
D)  carboxyl group

Question 15

Recombination between a plasmid and the cell chromosome will result in:

Accepted Answer

A)  Rapid degradation of the chromosome 
B)  Death of the cell 
C)  Immediate cellular replication 
D)  Stable integration of plasmid genes into the chromosome 
A)  Rapid degradation of the chromosome 
B)  Death of the cell 
C)  Immediate cellular replication 
D)  Stable integration of plasmid genes into the chromosome

Question 16

What type of bonds spontaneously form between two complementary strands of DNA?

Accepted Answer

A)  Hydrogen bonds 
B)  Covalent bonds 
C)  Phosphodiester bonds 
D)  Polar covalent bonds 
A)  Hydrogen bonds 
B)  Covalent bonds 
C)  Phosphodiester bonds 
D)  Polar covalent bonds

Question 17

DNA replication requires the presence of:

Accepted Answer

A)  DNA template 
B)  Pyrophosphate 
C)  Amino acids 
D)  Messenger RNA 
A)  DNA template 
B)  Pyrophosphate 
C)  Amino acids 
D)  Messenger RNA

Question 18

Which DNA sequence is complementary to 5F1F1F1S1F1F1F10GATTCTCAAAGGACT3F1F1F1S1F1F1F10?

Accepted Answer

A)  5F1F1F1S1F1F1F10GATTCTCAAAGGACT3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10GATTCTCAAAGGACT5F1F1F1S1F1F1F10 
C)  3F1F1F1S1F1F1F10CTAAGAGTTTCCTGA5F1F1F1S1F1F1F10 
D)  5F1F1F1S1F1F1F10CTAAGAGTTTCCTGA3F1F1F1S1F1F1F10 
A)  5F1F1F1S1F1F1F10GATTCTCAAAGGACT3F1F1F1S1F1F1F10 
B)  3F1F1F1S1F1F1F10GATTCTCAAAGGACT5F1F1F1S1F1F1F10 
C)  3F1F1F1S1F1F1F10CTAAGAGTTTCCTGA5F1F1F1S1F1F1F10 
D)  5F1F1F1S1F1F1F10CTAAGAGTTTCCTGA3F1F1F1S1F1F1F10

Question 19

Which of the following promote resistance to common antibiotics?

Accepted Answer

A)  Helicases 
B)  R factors 
C)  Endonucleases 
D)  Methylases 
A)  Helicases 
B)  R factors 
C)  Endonucleases 
D)  Methylases

Question 20

The term used to describe the arrangement of the individual strands in the double-stranded DNA molecule is:

Accepted Answer

A)  Parallel 
B)  Antiparallel 
C)  Tangential 
D)  Divergent 
A)  Parallel 
B)  Antiparallel 
C)  Tangential 
D)  Divergent

What reactions are catalyzed by DNA methyltransferase?

Nucleotides in DNA consist of which of the following?

The purpose of ligase is to:

The movement of DNA from one bacterium to another through the activity of bacteriophages is called:

DNA replication proceeds with the new daughter strand synthesized in the following orientation:

What is the fertility factor (F) observed by Hayes and Lederberg?

Which of the five ribose carbons of the deoxyribonucleotide are involved in formation of the DNA chain?

An exonuclease will catalyze what type of reaction?

Enzymes that recognize palindromic sequences of DNA, that are cut within the recognition sequence, that do not have methylating activity, and that are used frequently in the laboratory are which type of restriction enzymes?

In a double-stranded DNA molecule, base pairing between strands occurs between:

DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond?

The process of separating the two DNA strands into two single strands is called:

In DNA replication, the leading strand is the strand that has which orientation?

In a DNA molecule, one end has a free hydroxyl group and one end has a free ___________.

Recombination between a plasmid and the cell chromosome will result in:

What type of bonds spontaneously form between two complementary strands of DNA?

DNA replication requires the presence of:

Which DNA sequence is complementary to 5GATTCTCAAAGGACT3?

Which of the following promote resistance to common antibiotics?

The term used to describe the arrangement of the individual strands in the double-stranded DNA molecule is:

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

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Exam 1: DNA

What reactions are catalyzed by DNA methyltransferase?

Nucleotides in DNA consist of which of the following?

The purpose of ligase is to:

The movement of DNA from one bacterium to another through the activity of bacteriophages is called:

DNA replication proceeds with the new daughter strand synthesized in the following orientation:

What is the fertility factor (F) observed by Hayes and Lederberg?

Which of the five ribose carbons of the deoxyribonucleotide are involved in formation of the DNA chain?

An exonuclease will catalyze what type of reaction?

Enzymes that recognize palindromic sequences of DNA, that are cut within the recognition sequence, that do not have methylating activity, and that are used frequently in the laboratory are which type of restriction enzymes?

In a double-stranded DNA molecule, base pairing between strands occurs between:

DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond?

The process of separating the two DNA strands into two single strands is called:

In DNA replication, the leading strand is the strand that has which orientation?

In a DNA molecule, one end has a free hydroxyl group and one end has a free ___________.

Recombination between a plasmid and the cell chromosome will result in:

What type of bonds spontaneously form between two complementary strands of DNA?

DNA replication requires the presence of:

Which DNA sequence is complementary to 5GATTCTCAAAGGACT3?

Which of the following promote resistance to common antibiotics?

The term used to describe the arrangement of the individual strands in the double-stranded DNA molecule is:

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters