Question 1

HLA-A, HLA-B, and HLA-C are in what MHC region?

Accepted Answer

A)  Class I 
B)  Class II 
C)  Class III 
D)  Cytokine gene 
A)  Class I 
B)  Class II 
C)  Class III 
D)  Cytokine gene A

Question 2

What does the term HLA-DQ designate?

Accepted Answer

A)  HLA gene region, locus Q, sub-region D 
B)  The entire MHC gene region, locus D 
C)  HLA gene region, locus D, sub-region Q 
D)  The entire HLA-D locus 
A)  HLA gene region, locus Q, sub-region D 
B)  The entire MHC gene region, locus D 
C)  HLA gene region, locus D, sub-region Q 
D)  The entire HLA-D locus C

Question 3

In PCR-SSOP methods for HLA typing, the primers used to amplify the region are complementary to sequences present in which exon of the class II genes?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4 B

Question 4

In PCR-SSOP, the HLA allele is identified based on which of the following?

Accepted Answer

A)  Primers that are specific for particular alleles 
B)  Probes that are specific for particular alleles 
C)  Restriction enzyme cutting and band patterns 
D)  Differences in single-strand conformations 
A)  Primers that are specific for particular alleles 
B)  Probes that are specific for particular alleles 
C)  Restriction enzyme cutting and band patterns 
D)  Differences in single-strand conformations

Question 5

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

Accepted Answer

A)  HLA-D 
B)  mHag 
C)  KIR 
D)  HLA-A 
A)  HLA-D 
B)  mHag 
C)  KIR 
D)  HLA-A

Question 6

What is the relationship between the number of HLA alleles that have been identified by serological methods as compared with DNA analysis methods?

Accepted Answer

A)  There are fewer serologically defined alleles than DNA analysis-defined alleles 
B)  There are more serologically defined alleles than DNA analysis-defined alleles 
C)  There are equal numbers of serologically defined alleles as DNA analysis-defined alleles 
A)  There are fewer serologically defined alleles than DNA analysis-defined alleles 
B)  There are more serologically defined alleles than DNA analysis-defined alleles 
C)  There are equal numbers of serologically defined alleles as DNA analysis-defined alleles

Question 7

An HLA haplotype is inherited from:

Accepted Answer

A)  Only the mother 
B)  Only the father 
C)  Both the mother and the father 
D)  Neither the mother nor the father 
A)  Only the mother 
B)  Only the father 
C)  Both the mother and the father 
D)  Neither the mother nor the father

Question 8

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

Accepted Answer

A)  SBT 
B)  CDC 
C)  PCR-SSP 
D)  PCR-SSOP 
A)  SBT 
B)  CDC 
C)  PCR-SSP 
D)  PCR-SSOP

Question 9

Which of the following denotes a null allele in the HLA locus?

Accepted Answer

A)  A*25:02Q 
B)  DR*10:04N 
C)  B*44:01:01X 
D)  B*16:12-B*16:14 
A)  A*25:02Q 
B)  DR*10:04N 
C)  B*44:01:01X 
D)  B*16:12-B*16:14

Question 10

Polymorphisms in the HLA locus arise primarily through which type of genetic event?

Accepted Answer

A)  Translocation 
B)  Gene conversion 
C)  Missense mutation 
D)  Deletion 
A)  Translocation 
B)  Gene conversion 
C)  Missense mutation 
D)  Deletion

Question 11

In accepted HLA nomenclature for identifying HLA alleles by DNA sequence information, the fourth specific allele of the HLA-A31 family would be noted as which of the following?

Accepted Answer

A)  HLA-A*31:04 
B)  HLA-A*04:31 
C)  HLA-A*31-04 
D)  HLA-A*04:-1 
A)  HLA-A*31:04 
B)  HLA-A*04:31 
C)  HLA-A*31-04 
D)  HLA-A*04:-1

Question 12

A set of alleles on the same chromosome is called which of the following?

Accepted Answer

A)  Polymorphism 
B)  Aneuploid 
C)  Haplotype 
D)  Histocompatibility 
A)  Polymorphism 
B)  Aneuploid 
C)  Haplotype 
D)  Histocompatibility

Question 13

Which of the following methods used to determine HLA types always gives high-resolution results?

Accepted Answer

A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT 
A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT

Question 14

Which is the term for recognition of two or more antigens by the same antibody?

Accepted Answer

A)  Synonymous 
B)  Ambiguity 
C)  Nonsense 
D)  Null 
A)  Synonymous 
B)  Ambiguity 
C)  Nonsense 
D)  Null

Question 15

What are private epitopes?

Accepted Answer

A)  Molecules that bind to many different antibodies 
B)  Molecules only found on one individual 
C)  Molecules that bind to one specific HLA antibody type 
D)  Molecules that do not bind to any known HLA antibody 
A)  Molecules that bind to many different antibodies 
B)  Molecules only found on one individual 
C)  Molecules that bind to one specific HLA antibody type 
D)  Molecules that do not bind to any known HLA antibody

Question 16

A husband volunteers to be the donor for his wife's organ transplant. In pre-transplantation testing, the woman's serum was mixed in vitro with lymphocytes from her husband. After a few hours, complement and Trypan blue were added to the culture well. The cells were observed under the microscope and determined to be viable. What is the interpretation of this assay?

Accepted Answer

A)  The husband has antibodies that recognize his wife's cells. 
B)  The wife has antibodies that recognize her husband's cells. 
C)  The husband cannot donate his organ to his wife. 
D)  The husband can donate his organ to his wife. 
A)  The husband has antibodies that recognize his wife's cells. 
B)  The wife has antibodies that recognize her husband's cells. 
C)  The husband cannot donate his organ to his wife. 
D)  The husband can donate his organ to his wife.

Question 17

Sequence-specific PCR using allele-specific primers and primers for an amplification control has been performed on DNA isolated from a patient requiring bone marrow transplantation. Analysis of the reaction by gel electrophoresis reveals the presence of two bands in a gel lane. How is this interpreted?

Accepted Answer

A)  False positive 
B)  False negative 
C)  True positive 
D)  True negative 
A)  False positive 
B)  False negative 
C)  True positive 
D)  True negative

Question 18

Which is in the MHC class II region?

Accepted Answer

A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  HLA-D 
A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  HLA-D

Question 19

Which of the following molecular-based methods can be used to perform HLA typing?

Accepted Answer

A)  Southern blot 
B)  Sequencing 
C)  Single-strand conformation polymorphism 
D)  Pulsed field gel electrophoresis 
A)  Southern blot 
B)  Sequencing 
C)  Single-strand conformation polymorphism 
D)  Pulsed field gel electrophoresis

Question 20

DNA-based typing methods are used for which of the following?

Accepted Answer

A)  Determining serum antibody status 
B)  Crossmatching donors and recipients 
C)  Measuring T-cell-mediated cytotoxicity 
D)  Identifying HLA types 
A)  Determining serum antibody status 
B)  Crossmatching donors and recipients 
C)  Measuring T-cell-mediated cytotoxicity 
D)  Identifying HLA types

HLA-A, HLA-B, and HLA-C are in what MHC region?

What does the term HLA-DQ designate?

In PCR-SSOP methods for HLA typing, the primers used to amplify the region are complementary to sequences present in which exon of the class II genes?

In PCR-SSOP, the HLA allele is identified based on which of the following?

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

What is the relationship between the number of HLA alleles that have been identified by serological methods as compared with DNA analysis methods?

An HLA haplotype is inherited from:

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

Which of the following denotes a null allele in the HLA locus?

Polymorphisms in the HLA locus arise primarily through which type of genetic event?

In accepted HLA nomenclature for identifying HLA alleles by DNA sequence information, the fourth specific allele of the HLA-A31 family would be noted as which of the following?

A set of alleles on the same chromosome is called which of the following?

Which of the following methods used to determine HLA types always gives high-resolution results?

Which is the term for recognition of two or more antigens by the same antibody?

What are private epitopes?

Which is in the MHC class II region?

Which of the following molecular-based methods can be used to perform HLA typing?

DNA-based typing methods are used for which of the following?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 15: DNA-Based Tissue Typing

HLA-A, HLA-B, and HLA-C are in what MHC region?

What does the term HLA-DQ designate?

In PCR-SSOP methods for HLA typing, the primers used to amplify the region are complementary to sequences present in which exon of the class II genes?

In PCR-SSOP, the HLA allele is identified based on which of the following?

Mice with compromised immune systems are still capable of graft rejection due to what proteins?

What is the relationship between the number of HLA alleles that have been identified by serological methods as compared with DNA analysis methods?

An HLA haplotype is inherited from:

Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

Which of the following denotes a null allele in the HLA locus?

Polymorphisms in the HLA locus arise primarily through which type of genetic event?

In accepted HLA nomenclature for identifying HLA alleles by DNA sequence information, the fourth specific allele of the HLA-A31 family would be noted as which of the following?

A set of alleles on the same chromosome is called which of the following?

Which of the following methods used to determine HLA types always gives high-resolution results?

Which is the term for recognition of two or more antigens by the same antibody?

What are private epitopes?

Which is in the MHC class II region?

Which of the following molecular-based methods can be used to perform HLA typing?

DNA-based typing methods are used for which of the following?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

Filters