Question 1

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

Accepted Answer

A) Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA. 
B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size.This can take place with only a specific forward OR a specific reverse primer. 
C) No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs. 
D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes.Otherwise, you won't be able to detect the fragments that are made in the PCR process. 
A) Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA. 
B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size.This can take place with only a specific forward OR a specific reverse primer. 
C) No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs. 
D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes.Otherwise, you won't be able to detect the fragments that are made in the PCR process.

Question 2

A common vector used for cloning genes is/are

Accepted Answer

A) bacteria. 
B) viruses. 
C) nucleotides. 
D) plasmids. 
A) bacteria. 
B) viruses. 
C) nucleotides. 
D) plasmids.

Question 3

A DNA microarray contains oligonucleotides that contain a label.

Accepted Answer

A) True 
 B)False

Question 4

PCR is useful for amplifying a particular section of DNA.

Accepted Answer

A) True 
 B)False

Question 5

Digestion of DNA by restriction enzymes

Accepted Answer

A) produces sticky ends. 
B) produces blunt ends. 
C) cuts both strands of the DNA molecule. 
D) generates restriction fragments. 
E) All of the choices are correct. 
A) produces sticky ends. 
B) produces blunt ends. 
C) cuts both strands of the DNA molecule. 
D) generates restriction fragments. 
E) All of the choices are correct.

Question 6

FISH uses labeled probes to detect specific whole cells.

Accepted Answer

A) True 
 B)False

Question 7

Short tandem repeats (STRs)

Accepted Answer

A) are useful in identifying specific individuals 
B) are important sites in vectors where foreign DNA can be integrated 
C) are errors that can arise during DNA sequencing 
D) are DNA fragments generated during PCR 
A) are useful in identifying specific individuals 
B) are important sites in vectors where foreign DNA can be integrated 
C) are errors that can arise during DNA sequencing 
D) are DNA fragments generated during PCR

Question 8

DNA molecules that contain pieces of DNA from two different sources are defined as

Accepted Answer

A) biotechnology. 
B) gene cloning. 
C) recombinant DNA. 
D) genetic engineering. 
A) biotechnology. 
B) gene cloning. 
C) recombinant DNA. 
D) genetic engineering.

Question 9

The energy to separate fragments during agarose gel electrophoresis is supplied by

Accepted Answer

A) gravity. 
B) active transport. 
C) agarosis. 
D) electricity. 
A) gravity. 
B) active transport. 
C) agarosis. 
D) electricity.

Question 10

Which of the following genera has proved useful for manipulating plant cells?

Accepted Answer

A) Escherichia 
B) Bacillus 
C) Pseudomonas 
D) Agrobacterium 
A) Escherichia 
B) Bacillus 
C) Pseudomonas 
D) Agrobacterium

Question 11

The polymerase chain reaction is used to

Accepted Answer

A) amplify certain sections of DNA. 
B) amplify mRNA. 
C) produce proteins. 
D) produce long polymers of carbohydrates to be used in electrophoresis. 
A) amplify certain sections of DNA. 
B) amplify mRNA. 
C) produce proteins. 
D) produce long polymers of carbohydrates to be used in electrophoresis.

Question 12

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

Accepted Answer

A) red colonies. 
B) white colonies. 
C) blue colonies. 
D) All of the choices are correct. 
A) red colonies. 
B) white colonies. 
C) blue colonies. 
D) All of the choices are correct.

Question 13

The current cost of sequencing a human genome is about

Accepted Answer

A) $ 10,000 
B) $ 250,000 
C) $ 1,000,000 
D) $ 4,000,000 
A) $ 10,000 
B) $ 250,000 
C) $ 1,000,000 
D) $ 4,000,000

Exam 9: Biotechnology and Recombinant Dna

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

A common vector used for cloning genes is/are

A DNA microarray contains oligonucleotides that contain a label.

PCR is useful for amplifying a particular section of DNA.

Digestion of DNA by restriction enzymes

FISH uses labeled probes to detect specific whole cells.

Short tandem repeats (STRs)

DNA molecules that contain pieces of DNA from two different sources are defined as

The energy to separate fragments during agarose gel electrophoresis is supplied by

Which of the following genera has proved useful for manipulating plant cells?

The polymerase chain reaction is used to

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

The current cost of sequencing a human genome is about

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Dynamics of Prokaryotic Growth

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From Dna to Protein

Bacterial Genetics

Identification and Classification of Prokaryotes

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Infections

Nervous System Infections

Blood and Lymphatic Infections

Hiv Disease and Complications of Immunodeficiency

Microbial Ecology

Environmental Microbiology: Treatment of Water, Wastes, and Polluted Habitats

Food Microbiology

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