Question 1

Attenuation of the trp operon requires the presence of tryptophanyl-tRNATrp.

Accepted Answer

A) True 
 B)False

Question 2

Posttranscriptional controls of gene expression in bacteria often serve to fine-tune the amount of protein needed.

Accepted Answer

A) True 
 B)False

Question 3

In the regulation of the trp operon,tryptophan acts as a(n)

Accepted Answer

A)  repressor. 
B)  attenuator. 
C)  activator. 
D)  corepressor. 
E)  operator. 
A)  repressor. 
B)  attenuator. 
C)  activator. 
D)  corepressor. 
E)  operator.

Question 4

In the trp operon,the protein repressor alone cannot bind to the operator to be a negative regulator.

Accepted Answer

A) True 
 B)False

Question 5

There is only one site on the operator to which the lac repressor binds.

Accepted Answer

A) True 
 B)False

Question 6

In the operon model of gene regulation,transcription is shut off by the binding of the repressor to the promoter.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following combinations will produce the highest expression of ?-galactosidase? Assume crp- is a complete deletion of CRP (cAMP receptor protein).

Accepted Answer

A)  crp- on medium with glucose but no lactose 
B)  crp- on medium with lactose but no glucose 
C)  crp- on medium with both glucose and lactose 
D)  crp+ on medium with lactose but no glucose 
E)  crp+ on medium with both glucose and lactose 
A)  crp- on medium with glucose but no lactose 
B)  crp- on medium with lactose but no glucose 
C)  crp- on medium with both glucose and lactose 
D)  crp+ on medium with lactose but no glucose 
E)  crp+ on medium with both glucose and lactose

Question 8

You have isolated 15 mutant bacteria unable to use maltose.All 15 mutations map in the same region.You have tested the mutant for two enzymes in maltose metabolism and find at least one of the mutants lacks one of the enzymes,other mutants lack the other.What does this suggest?

Accepted Answer

A)  There are lots of enzymes in maltose metabolism. 
B)  All the genes for maltose metabolism are in a cluster and could be one operon. 
C)  There are genes scattered all over,we've simply found one set of mutants. 
D)  Maltose transport is very complex,these are transport mutants. 
E)  None of the choices are correct. 
A)  There are lots of enzymes in maltose metabolism. 
B)  All the genes for maltose metabolism are in a cluster and could be one operon. 
C)  There are genes scattered all over,we've simply found one set of mutants. 
D)  Maltose transport is very complex,these are transport mutants. 
E)  None of the choices are correct.

Question 9

How do positive regulators stimulate initiation of transcription by RNA polymerase?

Accepted Answer

A)  by allowing passage of the polymerase through the operator 
B)  by binding to the polymerase and increasing its ability to initiate transcription 
C)  by causing the helix to unwind in the operator allowing easier initiation 
D)  by making the transcription start site more exposed to the polymerase 
E)  by accelerating alpha helix formation 
A)  by allowing passage of the polymerase through the operator 
B)  by binding to the polymerase and increasing its ability to initiate transcription 
C)  by causing the helix to unwind in the operator allowing easier initiation 
D)  by making the transcription start site more exposed to the polymerase 
E)  by accelerating alpha helix formation

Question 10

Which of the following strains will be useful in demonstrating cis- regulation? In the genotypes listed below,the genes on the chromosome are on the left of the slash (/)symbol and the genes F' episome are on the right.

Accepted Answer

A)  lacI+ lacO+ lacZ+/lacI+ lacO+ lacZ+ 
B)  lacI+ lacOc lacZ+/lacI+ lacO+ lacZ- 
C)  lacI+ lacO+ lacZ+/lacI+ lacOc lacZ- 
D)  lacI+ lacO+ lacZ+/lacIs lacO+ lacZ- 
E)  lacIs lacO+ lacZ+/lacI+ lacO+ lacZ- 
A)  lacI+ lacO+ lacZ+/lacI+ lacO+ lacZ+ 
B)  lacI+ lacOc lacZ+/lacI+ lacO+ lacZ- 
C)  lacI+ lacO+ lacZ+/lacI+ lacOc lacZ- 
D)  lacI+ lacO+ lacZ+/lacIs lacO+ lacZ- 
E)  lacIs lacO+ lacZ+/lacI+ lacO+ lacZ-

Question 11

Negative regulation of gene expression usually takes place through enhancement of RNA polymerase activity.

Accepted Answer

A) True 
 B)False

Question 12

The critical step in the regulation of most bacterial genes occurs _____________.

Accepted Answer

A)  during transcription 
B)  at the release of mRNA 
C)  during translation 
D)  posttranslationally 
E)  during replication 
A)  during transcription 
B)  at the release of mRNA 
C)  during translation 
D)  posttranslationally 
E)  during replication

Question 13

The concept that proteins bind to DNA and regulate transcription holds true for both positive as well as negative regulation of gene expression.

Accepted Answer

A) True 
 B)False

Question 14

Describe the unique feature of the base sequence of the lac operator to which the lac repressor binds?

Accepted Answer

The lac repressor binds to a region of t

Question 15

Why is the lactose operon not fully induced in the presence of both lactose and glucose?

Accepted Answer

Glucose acts in cata

Question 16

Because there is no nuclear membrane in prokaryotes,transcription and translation from a single gene can be taking place at the same.

Accepted Answer

A) True 
 B)False

Question 17

An underlying principle of prokaryotic gene regulation is that it occurs through the binding of regulatory proteins to specific DNA sequences.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following combinations will produce the highest expression of β-galactosidase?

Accepted Answer

A)  lacI +on medium with lactose 
B)  lacIs on medium with lactose 
C)  lacI+ on medium with no lactose 
D)  lacIs on medium with no lactose 
E)  lacZ- on medium with no lactose 
A)  lacI +on medium with lactose 
B)  lacIs on medium with lactose 
C)  lacI+ on medium with no lactose 
D)  lacIs on medium with no lactose 
E)  lacZ- on medium with no lactose

Question 19

Global regulatory control of the heat-shock response in bacteria is achieved by the binding of σ70 to RNA polymerase.

Accepted Answer

A) True 
 B)False

Question 20

Explain what is meant by trans-acting and cis-acting elements.

Accepted Answer

Elements that act in trans can diffuse t

Exam 9: Genome Annotation

Attenuation of the trp operon requires the presence of tryptophanyl-tRNATrp.

Posttranscriptional controls of gene expression in bacteria often serve to fine-tune the amount of protein needed.

In the regulation of the trp operon,tryptophan acts as a(n)

In the trp operon,the protein repressor alone cannot bind to the operator to be a negative regulator.

There is only one site on the operator to which the lac repressor binds.

In the operon model of gene regulation,transcription is shut off by the binding of the repressor to the promoter.

Which of the following combinations will produce the highest expression of ?-galactosidase? Assume crp- is a complete deletion of CRP (cAMP receptor protein).

You have isolated 15 mutant bacteria unable to use maltose.All 15 mutations map in the same region.You have tested the mutant for two enzymes in maltose metabolism and find at least one of the mutants lacks one of the enzymes,other mutants lack the other.What does this suggest?

How do positive regulators stimulate initiation of transcription by RNA polymerase?

Which of the following strains will be useful in demonstrating cis- regulation? In the genotypes listed below,the genes on the chromosome are on the left of the slash (/)symbol and the genes F' episome are on the right.

Negative regulation of gene expression usually takes place through enhancement of RNA polymerase activity.

The critical step in the regulation of most bacterial genes occurs _____________.

The concept that proteins bind to DNA and regulate transcription holds true for both positive as well as negative regulation of gene expression.

Describe the unique feature of the base sequence of the lac operator to which the lac repressor binds?

Why is the lactose operon not fully induced in the presence of both lactose and glucose?

Because there is no nuclear membrane in prokaryotes,transcription and translation from a single gene can be taking place at the same.

An underlying principle of prokaryotic gene regulation is that it occurs through the binding of regulatory proteins to specific DNA sequences.

Which of the following combinations will produce the highest expression of β-galactosidase?

Global regulatory control of the heat-shock response in bacteria is achieved by the binding of σ70 to RNA polymerase.

Explain what is meant by trans-acting and cis-acting elements.

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters

Attenuation of the trp operon requires the presence of tryptophanyl-tRNA^Trp.

Which of the following combinations will produce the highest expression of ?-galactosidase? Assume crp^- is a complete deletion of CRP (cAMP receptor protein).

Global regulatory control of the heat-shock response in bacteria is achieved by the binding of σ⁷⁰ to RNA polymerase.