Exam 17: Genomics: Genetics From a Whole-Genome Perspective

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Explain the distinction between primary and secondary endosymbiosis.

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Primary endosymbiosis is when descendants of a free- living species without endosymbionts evolves into the endosymbiont of another species. Secondary endosymbiosis is where a free- living species with its own endosymbiont(s) is enveloped by a second host species. After a period of gene transfer, the endosymbionts from the once free- living species become the endosymbionts of the second host, resulting in the transfer of endosymbiont genomes (mitochondria or chloroplasts) across phylogenetically- distinct host taxa.

What are the closest "relatives" of chloroplasts still found as free- living organisms today?

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cyanobacteria

A consequence of organelle heredity is that sequence changes in mtDNA vary more than nuclear DNA in response to reduction in population size. Which of the following does this imply about human evolutionary history?

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If a chloroplast coding sequence has been transferred to the nuclear genome, but the protein product is needed in the chloroplast, what must be added to the original polypeptide sequence?

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In one or two words, give two characteristics of mitochondrial genomes.

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DNA transfer has occurred many times between organelle and nuclear genomes. Two pairs of nuclear and mitochondrial DNA sequences are analyzed. Pair A has more similarities than pair B. Which of these is the most ancient transfer?

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The noncoding regions of mammalian mitochondrial genomes have little to no selective pressure. Which of the following are consequences of this condition?

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The protein- coding genes of chloroplasts are involved in what part of photosynthesis?

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Is the rate of mutation in mitochondrial DNA of mammals greater or less than that of the species' nuclear DNA?

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When a cell divides, chloroplasts are randomly distributed. What is this called?

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How can you establish that, in a newly described species of plant, a particular phenotype is due to a chloroplast rather than a nuclear gene?

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Myoclonal epilepsy and ragged red fiber disease (MERRF) is a human condition named for the ragged red fibers of skeletal muscle cells and myoclonic epilepsy in affected individuals. People with this disorder have a mutation in a mitochondrial gene for a tRNA, specifically that for lysine. Affected individuals are heteroplastic. Why?

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The human egg has about 2000 mitochondrial genomes, but somatic cells have a range of hundreds to thousands. Which of the following is most likely to account for the difference?

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In mammals, the number of mitochondria per cell is highly variable. Which of the following would have the largest number: skin cells, red blood cells, or skeletal muscle cells?

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Which factor in organelle replication immediately precedes "organelle division"?

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Sequencing of genomes of eukaryotes, both mitochondrial and nuclear, have revealed evidence of sequence transfer from one to the other. Are such transfers ancient, recent, or both?

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In a plant, analysis of the Rubisco protein complex reveals that, in some complexes, rbcL is deficient while rbcS is normal. In other Rubisco complexes, both rbcL and rbcS are normal. What does this indicate about the two genes encoding these subunits?

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The plastids can differentiate into ___________that carry out photosynthesis.

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Variation in numbers of mutated chloroplast genomes can give rise to green, white, or variegated leaves. What color leaves do you expect in offspring of pollen from a white- leafed plant and ovules from a green- leafed plant?

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What is the name of the area of the organelle in which organelle DNA is packaged?

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