Exam 15: Analysis of Gene Function by Forward Genetics and Reverse Genetics

Snuppy, a male Afghan hound, was created by transferring the nucleus from an ear cell of the male Afghan hound, Tai, into an enucleated egg from a donor. The embryo was transplanted into a surrogate mother to develop to term. Which of the following animals would be a genetic match for Snuppy if nuclear DNA were tested?

Which of the following is FALSE regarding the formation of transgenic animals?

In selecting recombinant bacteria, cells are chosen that are resistant to a specific antibiotic. How are the bacteria made resistant?

If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?

You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with one enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp. Cutting the same DNA with another enzyme you recover 700 and 900 bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which of the following order of fragments, if any, best represents the restriction map?

Half of the palindromic restriction site that the endonuclease PstI recognizes, binds, and cleaves is 5'- CTG _______- 3'. What is the complete sequence?

Which of the following is a possible advantage for the environment of having farmers use plants that genetically produce Bt toxin?

Which of the following is true regarding the Cre- lox recombination system?

Why might the expression of a transgene be especially abnormal in vertebrates with larger average size of genes and amount of heterochromatin in their genomes?

What characteristics of plants allow them to be cloned relatively easily, whereas cloning in animals is rare and difficult to achieve?

Which of the following is FALSE regarding P element- mediated transformation?

Snuppy, a male Afghan hound, was created by transferring the nucleus from an ear cell of the male Afghan hound, Tai, into an enucleated egg from a donor. The embryo was transplanted into a surrogate mother to develop to term. Which of the following animals would be a genetic match for Snuppy if mitochondrial DNA were tested?

In species X, the UUU codon for phenylalanine is used significantly more frequently than other phenylalanine codons, and knowing this allows the scientist to manipulate translation efficiency. This unequal frequency is known as_________ .

If bacteria containing non- recombinant pUC18 are plated on X- gal medium, would colonies appear blue or white colonies or both?

You have isolated a plasmid from E. coli. You get the following results, using three restriction enzymes, 1 XhoI, 2 BamHI, and 3 EcoRI to perform six different digestions: single digests using each enzyme alone and double diges using each combination of two enzymes. enzyme \# fragments sizes (in kb) XhaI 1 10 BamHI 2 3.75,6.25 2 4.25,5.75 XhaI+BamHI 3 1.25,3.75,5 XhaI + 3 2,3.75,4.25 BamHI + 4 1.25,2.5,3,3.25 Draw a restriction map of the plasmid.

Cloning animals by nuclear implantation involves all of the following except?

Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s? I. Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading fra resulting in a fusion protein for each chain. II. Disulfide bonds were induced to form between the cysteine residues of the purified insulin A a chains. III. Both human insulin genes were reverse translated to create synthetic genes. IV. EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional cloning of each into a separate plasmid vector.

What technique of genomic library construction allows the genome to be cut by a given restriction enzyme at some but not all recognition sequences?

Which of the following is FALSE regarding grape cultivation?

What size fragments you would expect from an EcoRI/ XhoI double digest of the plasmid below?