Exam 20: Sexual Reproduction, Meiosis, and Genetic Recombination

Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduced into a strain of E. coli. To better the odds of getting expression of the eukaryotic gene in this system, you need to make certain that

Metaphase II arrest is mediated by , a biochemical activity present in mature eggs.

An individual has the genotype AaBbCcDdEe. How many different type of gametes can this individual make?

In studying T cell proliferation (cell division)in response to a specific antigen, you decide to stimulate these T cells with antigen and examine the genes that are activated by the interaction. To do this, following stimulation you should isolate

Fungi produce spores by which of the following processes?

You mate a squash with the genotype AAbb with a squash with the genotype aaBB. The resulting F1 squash are all AaBb. After mating two F1 squash, you obtain a F2 generation of squash as follows: A- B- 5 A- bb 45 aa B- 47 aa bb 3 total 100 Are A and B linked? Why or why not?

All of the following are capable of reproducing by parthenogenesis EXCEPT

When looking at a linear ascus of spores in Neurospora, you find the following octet: leu- leu- + + Leu- leu- leu- + This sequence of spores most likely arose as the result of

Genetic variability in sexually reproducing organisms arises within

The DNA of gene X in humans has been isolated, cloned, and sequenced and is 8000 base pairs in length. From cells that were actively transcribing gene X, a cDNA library was made. In this case, gene X was found to produce from a mRNA that was 4200 bases in length. The most probable explanation of these results is that

Charlie, an Angus bull, is heterozygous (Bb)for coat color, where B = black and b = brown. Charlie therefore has a black coat. Black is Charlie's

How many chromatids are present in prophase I of meiosis I in a diploid cell, 2n = 12?

Mitosis and meiosis differ in that

Mosses and ferns produce gametes by the process of

You purchase a black dog from a pet shop that tells you this animal is a purebred and that they will forward its papers to you at a later date. In this breed, black is dominant to white. You mate the dog with a white dog of the same breed. If the dog you purchased was a purebred animal, what approximate percentages of colors should you see in the offspring?

The synaptonemal complex forms in which subphase of prophase I of meiosis?

It is your task to prove the chromosomal theory of inheritance in your cell biology class. Your task is limited by examining only one trait in Drosophila. You may use the tools of modern biotechnology to aid you in your progress. Which of the following would be least likely to help you in your endeavor?

The alternative forms of a gene that can be expressed are known as

In bacterial cells, a cell that had the F plasmid integrated into the chromosome is known as

Homologous chromosomes are held together in the