Question 1

A functional protein microarray can be used to establish the function or substrates for a given group of
proteins.

Accepted Answer

A) True 
 B)False

Question 2

can be used to determine the amino acid sequence of a protein or peptide.

Accepted Answer

A)  X-ray crystallography 
B)  nuclear magnetic resonance 
C)  mass spectrometry 
D)  Chip assay 
A)  X-ray crystallography 
B)  nuclear magnetic resonance 
C)  mass spectrometry 
D)  Chip assay

Question 3

DNA microarray data may be used to determine the relationship between gene expression and protein abundance, however, these results must be confirmed by blot analysis.

Accepted Answer

A)  Western 
B)  Eastern 
C)  Northern 
D)  Southern 
A)  Western 
B)  Eastern 
C)  Northern 
D)  Southern

Question 4

Which of the following areas of genetics applies mathematical principles to genetic sequences in order to better understand the genetic information?

Accepted Answer

A)  Structural genomics 
B)  Functional genomics 
C)  Proteomics 
D)  Bioinformatics 
A)  Structural genomics 
B)  Functional genomics 
C)  Proteomics 
D)  Bioinformatics

Question 5

A cluster analysis is used when reading a DNA microarray to identify genes that appear to be
expressed together.

Accepted Answer

A) True 
 B)False

Question 6

The use of mathematics to understand the information within genetic sequences is called mathematical
genomics.

Accepted Answer

A) True 
 B)False

Question 7

What is the purpose of sodium dodecyl sulfate (SDS) in a two-dimensional gel electrophoresis experiment?

Accepted Answer

A)  Allows proteins to be separated by charge only 
B)  Allows proteins to be separated by size only 
C)  Neither of the above 
A)  Allows proteins to be separated by charge only 
B)  Allows proteins to be separated by size only 
C)  Neither of the above

Question 8

Proteome databases are collections of information on the genes of a specific species.

Accepted Answer

A) True 
 B)False

Question 9

Which of the following would be used with a protein microarray to establish protein expression?

Accepted Answer

A)  cDNA 
B)  mRNA 
C)  Plasmid vectors 
D)  Antibodies 
E)  None of the answers are correct 
A)  cDNA 
B)  mRNA 
C)  Plasmid vectors 
D)  Antibodies 
E)  None of the answers are correct

Question 10

What information would a subtractive cDNA library provide to a researcher?

Accepted Answer

A)  The total protein content of a cell 
B)  The number of introns in the genome 
C)  The mRNAs that are produced only under specific conditions 
D)  The total genes in an organism 
A)  The total protein content of a cell 
B)  The number of introns in the genome 
C)  The mRNAs that are produced only under specific conditions 
D)  The total genes in an organism

Question 11

Which of the following procedures is used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Accepted Answer

A)  Subtractive cDNA library 
B)  Treatment with reverse transcriptase to isolate the DNA of interest 
C)  Southern blotting 
D)  Mass spectrometry 
A)  Subtractive cDNA library 
B)  Treatment with reverse transcriptase to isolate the DNA of interest 
C)  Southern blotting 
D)  Mass spectrometry

Question 12

Which of the following is used to separate cellular proteins for analysis?

Accepted Answer

A)  Western blots 
B)  Subtractive cDNA libraries 
C)  Two-dimensional gel electrophoresis 
D)  Pulsed field gel electrophoresis 
E)  None of the answers are correct 
A)  Western blots 
B)  Subtractive cDNA libraries 
C)  Two-dimensional gel electrophoresis 
D)  Pulsed field gel electrophoresis 
E)  None of the answers are correct

Question 13

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by .

Accepted Answer

A)  Net charge 
B)  Molecular weight 
C)  Abundance 
D)  None of the answers are correct 
A)  Net charge 
B)  Molecular weight 
C)  Abundance 
D)  None of the answers are correct

Question 14

The most common mechanism by which the proteome varies from the genome is

Accepted Answer

A)  Alternative splicing 
B)  Posttranslational covalent modification 
C)  RNA editing 
D)  None of the answers are correct 
A)  Alternative splicing 
B)  Posttranslational covalent modification 
C)  RNA editing 
D)  None of the answers are correct

Question 15

To identify novel DNA binding sites for a protein, it would be best to use:

Accepted Answer

A)  BLAST 
B)  YACs 
C)  Chromatin immunoprecipitation 
D)  ChIP on chip 
E)  Time shift experiments 
A)  BLAST 
B)  YACs 
C)  Chromatin immunoprecipitation 
D)  ChIP on chip 
E)  Time shift experiments

Question 16

Two genes that share a common ancestry are said to have homology.

Accepted Answer

A) True 
 B)False

Question 17

Genes are to the genome as are to the proteome.

Accepted Answer

A)  carbohydrates 
B)  proteins 
C)  DNA 
D)  prions 
A)  carbohydrates 
B)  proteins 
C)  DNA 
D)  prions

Question 18

Which of the following terms best describes two homologous genes found in different species?

Accepted Answer

A)  Homologues 
B)  Paralogs 
C)  Gene family 
D)  Orthologs 
E)  None of the answers are correct 
A)  Homologues 
B)  Paralogs 
C)  Gene family 
D)  Orthologs 
E)  None of the answers are correct

Question 19

cDNA uses chromosomal DNA as its template.

Accepted Answer

A) True 
 B)False

Question 20

In the screening of a DNA microarray, detection of a fluorescent signal from one of the genes indicates which of the following?

Accepted Answer

A)  The probe hybridized to that particular gene 
B)  The probe did not hybridize to that particular gene 
C)  The experiment was not conducted correctly 
D)  None of the answers are correct 
A)  The probe hybridized to that particular gene 
B)  The probe did not hybridize to that particular gene 
C)  The experiment was not conducted correctly 
D)  None of the answers are correct

Exam 21: Genomics II: Functional Genomics, Proteomics, and

A functional protein microarray can be used to establish the function or substrates for a given group of proteins.

can be used to determine the amino acid sequence of a protein or peptide.

DNA microarray data may be used to determine the relationship between gene expression and protein abundance, however, these results must be confirmed by blot analysis.

Which of the following areas of genetics applies mathematical principles to genetic sequences in order to better understand the genetic information?

A cluster analysis is used when reading a DNA microarray to identify genes that appear to be expressed together.

The use of mathematics to understand the information within genetic sequences is called mathematical genomics.

What is the purpose of sodium dodecyl sulfate (SDS) in a two-dimensional gel electrophoresis experiment?

Proteome databases are collections of information on the genes of a specific species.

Which of the following would be used with a protein microarray to establish protein expression?

What information would a subtractive cDNA library provide to a researcher?

Which of the following procedures is used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Which of the following is used to separate cellular proteins for analysis?

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by .

The most common mechanism by which the proteome varies from the genome is

To identify novel DNA binding sites for a protein, it would be best to use:

Two genes that share a common ancestry are said to have homology.

Genes are to the genome as are to the proteome.

Which of the following terms best describes two homologous genes found in different species?

cDNA uses chromosomal DNA as its template.

In the screening of a DNA microarray, detection of a fluorescent signal from one of the genes indicates which of the following?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Biotechnology

Genomics I: Analysis of Dna

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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