Question 1

After being cut with the same restriction enzyme, what cellular enzyme is used to link the selected gene and the cloning vector?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  DNA helicase 
A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  DNA helicase

Question 2

A short sequence with many sites where restriction enzymes may cut.

Accepted Answer

A)  Cloning vector 
B)  Multiple cloning site 
C)  Restriction enzyme site 
D)  Origin sequence 
A)  Cloning vector 
B)  Multiple cloning site 
C)  Restriction enzyme site 
D)  Origin sequence

Question 3

The three fundamental functions of genetic material in a cell include ________. (Select all that apply)

Accepted Answer

A)  storage of information needed for making all the parts of a living organism 
B)  accurate replication of the information so that it may be passed on to the next generation 
C)  opportunity for genetic variation to increase diversity in populations so that they will have a chance to survive environmental change 
D)  to have enzymatic activity necessary for the metabolism of the cell 
A)  storage of information needed for making all the parts of a living organism 
B)  accurate replication of the information so that it may be passed on to the next generation 
C)  opportunity for genetic variation to increase diversity in populations so that they will have a chance to survive environmental change 
D)  to have enzymatic activity necessary for the metabolism of the cell

Question 4

The white colonies in the illustration below ________.

Accepted Answer

A)  do not produce a functional beta-galactosidase protein so the clear lactose analog is not converted to a blue-colored product 
B)  produce an active beta-galactosidase protein that does not hydrolyze the blue-colored lactose analog 
C)  naturally produces a blue pigment which is masked by the gram positive cell wall 
D)  produce lysozyme which breaks open the cells to release an inner white pigment 
A)  do not produce a functional beta-galactosidase protein so the clear lactose analog is not converted to a blue-colored product 
B)  produce an active beta-galactosidase protein that does not hydrolyze the blue-colored lactose analog 
C)  naturally produces a blue pigment which is masked by the gram positive cell wall 
D)  produce lysozyme which breaks open the cells to release an inner white pigment

Question 5

mRNA ________.

Accepted Answer

A)  is transcribed from DNA template strand, coding molecules are transcribed into proteins 
B)  participates in various regulatory functions 
C)  is a structural components of ribosomes 
D)  is involved in translation by carrying amino acids to ribosomes 
A)  is transcribed from DNA template strand, coding molecules are transcribed into proteins 
B)  participates in various regulatory functions 
C)  is a structural components of ribosomes 
D)  is involved in translation by carrying amino acids to ribosomes

Question 6

The terminator sequence is the ________.

Accepted Answer

A)  binding site for enzymes that bind RNA polymerase to DNA, separate strands of DNA and select template strand 
B)  RNA sequence that binds factors to stop translation and release newly formed mRNA complex 
C)  RNA sequence that binds factors to stop transcription and release newly formed mRNA complex 
D)  DNA sequence that binds factors to stop transcription and release newly formed mRNA complex 
A)  binding site for enzymes that bind RNA polymerase to DNA, separate strands of DNA and select template strand 
B)  RNA sequence that binds factors to stop translation and release newly formed mRNA complex 
C)  RNA sequence that binds factors to stop transcription and release newly formed mRNA complex 
D)  DNA sequence that binds factors to stop transcription and release newly formed mRNA complex

Question 7

Gene regulation in Eukaryotes include(s) ________. (Select all that apply)

Accepted Answer

A)  packaging of DNA into tight coils around the histone proteins to activate transcription 
B)  degrading mRNA 
C)  processing proteins to an active form by cleavage 
D)  removal of introns and splicing together of exons 
A)  packaging of DNA into tight coils around the histone proteins to activate transcription 
B)  degrading mRNA 
C)  processing proteins to an active form by cleavage 
D)  removal of introns and splicing together of exons

Question 8

What is the correct order of steps in the process of cloning a Eukaryotic gene and inserting it into a plasmid?
1) isolate the spliced mRNA that produces the desired protein
2) synthesize a cDNA using reverse transcriptase
3) transform a bacteria and select for appropriate antibiotic resistance
4) insert the cDNA into a cloning vector to form recombinant DNA

Accepted Answer

A)  1, 2, 4, 3 
B)  1, 2, 3, 4 
C)  1, 4, 2, 3 
D)  1, 3, 2, 4 
A)  1, 2, 4, 3 
B)  1, 2, 3, 4 
C)  1, 4, 2, 3 
D)  1, 3, 2, 4

Question 9

The DNA sequence for enzymes that bind RNA polymerase to DNA, separate strands of DNA and select template strand ________.

Accepted Answer

A)  promoter 
B)  terminator 
C)  coding strand 
D)  template strand 
A)  promoter 
B)  terminator 
C)  coding strand 
D)  template strand

Question 10

Based on the STR results, what is the correct conclusion for the paternity of the child? (Select all that apply)

Accepted Answer

A)  The mother does not have enough matching STR alleles to be the mother of the child. 
B)  The father does not have enough matching STR alleles to be the father of the child. 
C)  The matching STR allele for each locus is consistent with this woman being the mother of the child. 
D)  The matching STR allele for each locus is consistent with this man being the father of the child. 
A)  The mother does not have enough matching STR alleles to be the mother of the child. 
B)  The father does not have enough matching STR alleles to be the father of the child. 
C)  The matching STR allele for each locus is consistent with this woman being the mother of the child. 
D)  The matching STR allele for each locus is consistent with this man being the father of the child.

Question 11

What stage of the elongation of protein synthesis is shown in the illustration?

Accepted Answer

A)  Aminoacyl-tRNA selection 
B)  Translocation 
C)  Peptide transfer 
D)  Removal of an unacylated tRNA from the E site 
A)  Aminoacyl-tRNA selection 
B)  Translocation 
C)  Peptide transfer 
D)  Removal of an unacylated tRNA from the E site

Question 12

A structural component of ribosomes ________.

Accepted Answer

A)  mRNA 
B)  tRNA 
C)  rRNA 
D)  miRNA 
A)  mRNA 
B)  tRNA 
C)  rRNA 
D)  miRNA

Question 13

The Ames Test is a procedure that ________.

Accepted Answer

A)  tests the capability of a mutagen to produce reversion mutations 
B)  assesses the ability of methionine-requiring strains 
C)  analyzes the mutagenic ability of E. coli 
D)  screens for the presence of methionine 
A)  tests the capability of a mutagen to produce reversion mutations 
B)  assesses the ability of methionine-requiring strains 
C)  analyzes the mutagenic ability of E. coli 
D)  screens for the presence of methionine

Question 14

The genetic code ________. (Select all that apply)

Accepted Answer

A)  is contained in codon sequences found in DNA 
B)  is redundant because there is often more than one choice of codon for each amino acid 
C)  uses AUG coding for Methionine as the start of every protein 
D)  uses one of three codons that do not code for an amino acid to stand for "stop" at the end of the protein 
A)  is contained in codon sequences found in DNA 
B)  is redundant because there is often more than one choice of codon for each amino acid 
C)  uses AUG coding for Methionine as the start of every protein 
D)  uses one of three codons that do not code for an amino acid to stand for "stop" at the end of the protein

Question 15

In the illustration below, the item marked v is ________.

Accepted Answer

A)  the coding strand of DNA 
B)  the template strand of DNA 
C)  an Okazaki fragment of DNA 
D)  the protein coding region of a gene 
A)  the coding strand of DNA 
B)  the template strand of DNA 
C)  an Okazaki fragment of DNA 
D)  the protein coding region of a gene

Question 16

How has biotechnology improved our lives? (Select all that apply)

Accepted Answer

A)  Production of industrial enzymes used in detergents, paper production and food industry. 
B)  The use of DNA profiling techniques for paternity identification and crime scene investigation. 
C)  Development of herbicide and pest-resistant crop plants. 
D)  Production of needed pharmaceutical products. 
A)  Production of industrial enzymes used in detergents, paper production and food industry. 
B)  The use of DNA profiling techniques for paternity identification and crime scene investigation. 
C)  Development of herbicide and pest-resistant crop plants. 
D)  Production of needed pharmaceutical products.

Question 17

Restriction enzymes ________. (Select all that apply)

Accepted Answer

A)  are used to cut DNA at specific nucleotide sequences 
B)  DNA sequences where restriction enzyme cut are often palindromic 
C)  only cut DNA at one site on a chromosome or plasmid 
D)  often cut the DNA strand asymmetrically so that short single-stranded sticky ends are formed 
A)  are used to cut DNA at specific nucleotide sequences 
B)  DNA sequences where restriction enzyme cut are often palindromic 
C)  only cut DNA at one site on a chromosome or plasmid 
D)  often cut the DNA strand asymmetrically so that short single-stranded sticky ends are formed

Question 18

The enzyme used to make a cDNA from a eukaryotic mRNA is ________.

Accepted Answer

A)  reverse DNA polymerase 
B)  Taq thermostable DNA polymerase 
C)  reverse transcriptase 
D)  RNA primase 
A)  reverse DNA polymerase 
B)  Taq thermostable DNA polymerase 
C)  reverse transcriptase 
D)  RNA primase

Question 19

Genomics is ________.

Accepted Answer

A)  the study of the structure and function of an entire genome and its interrelated metabolic cellular pathways 
B)  the study of heredity and the variation of inherited characteristics 
C)  the study of the genetic material of all the microbes that live on and inside the human body 
D)  the study of noncoding DNA, as well as mitochondrial DNA 
A)  the study of the structure and function of an entire genome and its interrelated metabolic cellular pathways 
B)  the study of heredity and the variation of inherited characteristics 
C)  the study of the genetic material of all the microbes that live on and inside the human body 
D)  the study of noncoding DNA, as well as mitochondrial DNA

Question 20

A microbiome is ________.

Accepted Answer

A)  multiple genes and sets of related genes in used in cell metabolism or organismal development 
B)  the structure and function of an entire genome and its interrelated metabolic cellular pathways 
C)  the sequence of DNA used in DNA profiling 
D)  the combined genomes of organisms in a microbial community 
A)  multiple genes and sets of related genes in used in cell metabolism or organismal development 
B)  the structure and function of an entire genome and its interrelated metabolic cellular pathways 
C)  the sequence of DNA used in DNA profiling 
D)  the combined genomes of organisms in a microbial community

Exam 10: Microbial Genetics and Genetic Engineering

After being cut with the same restriction enzyme, what cellular enzyme is used to link the selected gene and the cloning vector?

A short sequence with many sites where restriction enzymes may cut.

The three fundamental functions of genetic material in a cell include ________. (Select all that apply)

The white colonies in the illustration below ________.

mRNA ________.

The terminator sequence is the ________.

Gene regulation in Eukaryotes include(s) ________. (Select all that apply)

The DNA sequence for enzymes that bind RNA polymerase to DNA, separate strands of DNA and select template strand ________.

Based on the STR results, what is the correct conclusion for the paternity of the child? (Select all that apply)

What stage of the elongation of protein synthesis is shown in the illustration?

A structural component of ribosomes ________.

The Ames Test is a procedure that ________.

The genetic code ________. (Select all that apply)

In the illustration below, the item marked v is ________.

How has biotechnology improved our lives? (Select all that apply)

Restriction enzymes ________. (Select all that apply)

The enzyme used to make a cDNA from a eukaryotic mRNA is ________.

Genomics is ________.

A microbiome is ________.

Microbial World

An Introduction to the Chemical Basis of Life

The Biochemistry of Macromolecules

Microscopy

Prokaryote Organisms

Eukaryotic Cells

Eukaryotic Organisms

Viruses and Infectious Particles

Metabolism

Microbial Growth and Control

Antimicrobial Agents

Innate Immunity

Adaptive Immunity

Vaccination, Immunoassays, and Immune Disorders

Microbial Pathogenesis

Epidemiology and Infection Control

Diseases of the Respiratory System

Diseases of the Skin and Eyes

Diseases of the Gastrointestinal System

Diseases of the Urogenital System

Diseases of the Nervous System

Diseases of the Cardiovascular and Lymphatic Systems

Environmental and Industrial Microbiology

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