Exam 13: An Introduction to Genetic Technology

A restriction enzyme is most comparable to a ___________.

Recombinant vectors are often propagated in bacterial cells.

Recall that human and other eukaryotic genes usually contain introns (untranslated segments), whereas bacterial (prokaryotic) genes do not. What problem does this create if we wish to transfer a human gene into a bacterium for production of a useful protein product?

Restriction enzymes recognize and cut DNA at specific nucleotide sequences.

The cloning technique that employs in vitro fertilization is ____________.

Embryo splitting is done when the embryo contains ____________________ to ____________________ cells.

In recombinant DNA technology, _________ are used to cut DNA at specific sites.

Plasmids are short sequences of DNA used as primers in PCR.

In Southern blotting, DNA fragments on filters must be ____________________ so that probes can bond with them.

In the PCR technique, what bonds are broken when DNA is heated?

Southern blotting is a technique for analyzing DNA.

Finding a specific clone in a library does NOT require

Explain what restriction enzymes do to a strand of DNA and how they are used in recombinant DNA experiments.

Restriction enzymes

The vectors most commonly used in recombinant DNA technology are ____________.

In the DNA sequencing procedure, each of the four nucleotides is distinguished from the others by

To find a specific DNA sequence in a genomic library, researchers employ single-stranded RNA or DNA molecules called _________.

The polymerase chain reaction

Heat is required for the polymerase chain reaction.

A method for amplifying DNA segments using DNA polymerase is ____________.