Question 1

DNA helicase inhibitors are well studied as potential drug targets.What would you expect to see if DNA helicase activity is inhibited?

Accepted Answer

A) The replisome complex would not assemble on the oriC region. 
B) The DNA cannot bend,so hydrogen bonds in the 13-mer region of oriC remain intact. 
C) Helicase catalyzes ATP hydrolysis and DNA strands separation,so the helix cannot be unwound and strands will not separate. 
D) Helicase carries the SSB protein to the open region of DNA,so hydrolysis and strand separation will not occur. 
E) Helicase prevents reannealing of the separated strands,so strands would quickly reanneal and DNA replication cannot proceed. 
A) The replisome complex would not assemble on the oriC region. 
B) The DNA cannot bend,so hydrogen bonds in the 13-mer region of oriC remain intact. 
C) Helicase catalyzes ATP hydrolysis and DNA strands separation,so the helix cannot be unwound and strands will not separate. 
D) Helicase carries the SSB protein to the open region of DNA,so hydrolysis and strand separation will not occur. 
E) Helicase prevents reannealing of the separated strands,so strands would quickly reanneal and DNA replication cannot proceed.

Question 2

What are the three steps of each PCR cycle?

Accepted Answer

denaturati

Question 3

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA,a function of the enzyme's

Accepted Answer

A) 3′-to-5′ exonuclease activity. 
B) 5′-to-3′ exonuclease activity. 
C) 3′-to-5′ polymerase activity. 
D) 5′-to-3′ polymerase activity. 
E) 3′-to-5′ helicase activity. 
A) 3′-to-5′ exonuclease activity. 
B) 5′-to-3′ exonuclease activity. 
C) 3′-to-5′ polymerase activity. 
D) 5′-to-3′ polymerase activity. 
E) 3′-to-5′ helicase activity.

Question 4

What types of bonds are formed between complementary DNA bases?

Accepted Answer

A) glycosidic bonds 
B) phosphodiester bonds 
C) covalent bonds 
D) hydrogen bonds 
E) ionic bonds 
A) glycosidic bonds 
B) phosphodiester bonds 
C) covalent bonds 
D) hydrogen bonds 
E) ionic bonds

Question 5

Which of these components would you use to distinguish whether a sample has been prepared for a Sanger sequencing reaction or for a polymerase chain reaction?

Accepted Answer

A) DNA template 
B) DNA polymerase 
C) dNTPs 
D) ddNTPs 
E) DNA primer 
A) DNA template 
B) DNA polymerase 
C) dNTPs 
D) ddNTPs 
E) DNA primer

Question 6

In 1928,Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

Accepted Answer

A) living type RII 
B) heat-killed type SIII 
C) heat-killed type RII 
D) heat-killed type SIII and living type RII 
E) heat-killed type RII and living type RII 
A) living type RII 
B) heat-killed type SIII 
C) heat-killed type RII 
D) heat-killed type SIII and living type RII 
E) heat-killed type RII and living type RII

Question 7

During DNA replication in E.coli,what is the role of DnaA?

Accepted Answer

A) DnaA is a region of DNA recognized by the replisome where replication is initiated. 
B) DnaA bends DNA,hydrolyzing the hydrogen bonds in the 13-mer region of oriC and creating an open complex. 
C) DnaA is a helicase that uses ATP to hydrolyze hydrogen bonds and separate DNA strands. 
D) DnaA carries the helicase protein to the open region of DNA where hydrolysis and strand separation will occur. 
E) DnaA prevents reannealing of the separated strands so replication can proceed. 
A) DnaA is a region of DNA recognized by the replisome where replication is initiated. 
B) DnaA bends DNA,hydrolyzing the hydrogen bonds in the 13-mer region of oriC and creating an open complex. 
C) DnaA is a helicase that uses ATP to hydrolyze hydrogen bonds and separate DNA strands. 
D) DnaA carries the helicase protein to the open region of DNA where hydrolysis and strand separation will occur. 
E) DnaA prevents reannealing of the separated strands so replication can proceed.

Question 8

What are the three parts of a DNA nucleotide?

Accepted Answer

deoxyribos

Question 9

________ is the term for multiprotein complexes in which a core enzyme is associated with additional protein components leading to its function.

Accepted Answer

The answer of ________ is the term for multiprotein complexes...

DNA helicase inhibitors are well studied as potential drug targets.What would you expect to see if DNA helicase activity is inhibited?

What are the three steps of each PCR cycle?

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA,a function of the enzyme's

What types of bonds are formed between complementary DNA bases?

Which of these components would you use to distinguish whether a sample has been prepared for a Sanger sequencing reaction or for a polymerase chain reaction?

In 1928,Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

During DNA replication in E.coli,what is the role of DnaA?

What are the three parts of a DNA nucleotide?

________ is the term for multiprotein complexes in which a core enzyme is associated with additional protein components leading to its function.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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Exam 7: DNA Structure and Replication

DNA helicase inhibitors are well studied as potential drug targets.What would you expect to see if DNA helicase activity is inhibited?

What are the three steps of each PCR cycle?

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA,a function of the enzyme's

What types of bonds are formed between complementary DNA bases?

Which of these components would you use to distinguish whether a sample has been prepared for a Sanger sequencing reaction or for a polymerase chain reaction?

In 1928,Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

During DNA replication in E.coli,what is the role of DnaA?

What are the three parts of a DNA nucleotide?

________ is the term for multiprotein complexes in which a core enzyme is associated with additional protein components leading to its function.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters