Exam 15: Regulation of Gene Expression in Eukaryotes

Regulatory regions of a eukaryotic gene all contain which of the following sequences,which act only on one copy of the chromosome?

Inherited mutations in the Xist promoter lead to defects in which epigenetic process?

In yeast,which gene would you expect to be activated during phosphate starvation?

Which protein complex directs DNA bending into loops that contact RNA polymerase and transcription factors bound at the core promoter or with protein complexes bound to proximal promoter elements?

The imprinting control region (ICR)involved in genomic imprinting on chromosome 15 is what type of sequence?

In animals,the ________ enzyme cuts pri-mRNA to produce mi-RNA.

Prader-Willi syndrome is a genetic disorder involving a partial deletion of chromosome 15q on the paternal chromosome.When both copies of a gene (or chromosome)are functional but only one is expressed,this is an example of

A region of chromatin has recently become DNAse I hypersensitive.Which enzyme has been activated to cause this change in chromatin structure?

How does transcription inhibition differ between prokaryotes and eukaryotes? Describe the two mechanisms used by eukaryotes to direct the enhancers toward certain promoters and away from others.

Which sequences would likely have both a nucleosome-depleted region (NDR)and a poly A/T tract to attract transcriptional activators to a transcription start sequence?

What are three proteins you would expect to find bound to the core promoter region in eukaryotes?

In the GAL gene system,which protein acts as an activator protein through its transcription-initiating effect?

In the GAL gene system,________ are cis-acting regulatory elements,and ________ protein is a trans-acting regulatory protein.

Many types of cancer are known to overexpress the receptor protein tyrosine kinase.Which molecular technique can be used to reduce expression of an oncogene in vitro?

If you want to affect chromatin packaging,which amino acid could you mutate to affect both histone acetylation and methylation patterns?

In the presence of galactose,you unexpectedly find that transcription is still blocked.Assuming Gal80 is present and functional,which other protein may be mutated to prevent transcriptional activation?

Demethylation and acetylation lead to open chromatin structure and are associated with ________ regions of genomes.

Chromatin remodeling involves both sliding and relocating of the nucleosomes.Which eukaryotic chromatin remodeling complex is likely involved?

In gal⁺ yeast,what is the product of the galactose utilization pathway?

Which protein binds to the silencer sequence and promotes transcriptional silencing in the presence of glucose?