Question 1

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80.What effect would you expect to see in the absence of galactose?

Accepted Answer

transcript

Question 2

Which protein complex binds dsRNA fragments to generate ssRNAs for RNAi?

Accepted Answer

RNA-induce

Question 3

Which of the four nucleotides are typically methylated in "islands," resulting in closed chromatin structure?

Accepted Answer

The answer of Which of the four nucleotides are typically...

Question 4

For the following gene,MspI and HpaII restriction sites and their positions are indicated here.Recall that HpaII is methylation sensitive,while MspI is not.  
If this gene is being actively transcribed,how many fragments would you expect from this restriction digest?

Accepted Answer

A) 1 
B) 3 
C) 4 
D) 5 
E) 6 
A) 1 
B) 3 
C) 4 
D) 5 
E) 6

Question 5

When CpG islands are unmethylated,

Accepted Answer

A) chromatin in the promoter region is open,allowing access by transcription factors and RNA polymerase. 
B) chromatin in the promoter region is closed,preventing transcription factors and RNA polymerase from binding. 
C) chromatin in the enhancer region is closed,so they are unable to bind regulatory proteins to initiate transcription. 
D) DNAse hypersensitivity in that region of the chromosome is lost. 
E) genes downstream of the CpG islands cannot be expressed,because the promoter region is blocked by histones. 
A) chromatin in the promoter region is open,allowing access by transcription factors and RNA polymerase. 
B) chromatin in the promoter region is closed,preventing transcription factors and RNA polymerase from binding. 
C) chromatin in the enhancer region is closed,so they are unable to bind regulatory proteins to initiate transcription. 
D) DNAse hypersensitivity in that region of the chromosome is lost. 
E) genes downstream of the CpG islands cannot be expressed,because the promoter region is blocked by histones.

Question 6

In yeast,if you want to prevent the Gal4 regulatory protein from binding near each of the GAL genes,which sequence element would you target?

Accepted Answer

A) core promoter 
B) proximal elements 
C) enhancer sequence 
D) homeodomain motif 
E) upstream activator sequence 
A) core promoter 
B) proximal elements 
C) enhancer sequence 
D) homeodomain motif 
E) upstream activator sequence

Question 7

For the following gene,which type of regulatory sequence has likely been deleted in mutant 1?

Accepted Answer

The answer of For the following gene,which type of regulatory...

Question 8

For the following gene,you notice the following results.
 
What type of sequence has been mutated in mutant 4?

Accepted Answer

tissue-spe

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80.What effect would you expect to see in the absence of galactose?

Which protein complex binds dsRNA fragments to generate ssRNAs for RNAi?

Which of the four nucleotides are typically methylated in "islands," resulting in closed chromatin structure?

For the following gene,MspI and HpaII restriction sites and their positions are indicated here.Recall that HpaII is methylation sensitive,while MspI is not. If this gene is being actively transcribed,how many fragments would you expect from this restriction digest?

When CpG islands are unmethylated,

In yeast,if you want to prevent the Gal4 regulatory protein from binding near each of the GAL genes,which sequence element would you target?

For the following gene,which type of regulatory sequence has likely been deleted in mutant 1?

For the following gene,you notice the following results. What type of sequence has been mutated in mutant 4?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophage

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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Exam 15: Regulation of Gene Expression in Eukaryotes

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80.What effect would you expect to see in the absence of galactose?

Which protein complex binds dsRNA fragments to generate ssRNAs for RNAi?

Which of the four nucleotides are typically methylated in "islands," resulting in closed chromatin structure?

For the following gene,MspI and HpaII restriction sites and their positions are indicated here.Recall that HpaII is methylation sensitive,while MspI is not. If this gene is being actively transcribed,how many fragments would you expect from this restriction digest?

When CpG islands are unmethylated,

In yeast,if you want to prevent the Gal4 regulatory protein from binding near each of the GAL genes,which sequence element would you target?

For the following gene,which type of regulatory sequence has likely been deleted in mutant 1?

For the following gene,you notice the following results. What type of sequence has been mutated in mutant 4?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophage

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters