Question 1

Which of the following best describes the function of the RecA protein in E.coli?

Accepted Answer

A)  Promotes strand invasion and D-loop formation in the double-stranded break model 
B)  Recognizes double-stranded breaks in the DNA 
C)  Involved in the initial formation of Holliday junctions 
D)  Resolves Holliday junctions into separate chromosomes 
A)  Promotes strand invasion and D-loop formation in the double-stranded break model 
B)  Recognizes double-stranded breaks in the DNA 
C)  Involved in the initial formation of Holliday junctions 
D)  Resolves Holliday junctions into separate chromosomes

Question 2

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Accepted Answer

A)  5′ to 3′ 
B)  3′ to 5′ 
C)  They travel in both directions. 
D)  They remain stationary. 
A)  5′ to 3′ 
B)  3′ to 5′ 
C)  They travel in both directions. 
D)  They remain stationary.

Question 3

Homologous recombination that leads to genetic diversity occurs during meiosis II.

Accepted Answer

A) True 
 B)False

Question 4

Recombination between sister chromatids produces new combinations of alleles that may be beneficial to the species.

Accepted Answer

A) True 
 B)False

Question 5

The proofreading of the DNA occurs in the ________.

Accepted Answer

A)  5′ to 3′ direction 
B)  3′ to 5′ direction 
C)  both directions 
A)  5′ to 3′ direction 
B)  3′ to 5′ direction 
C)  both directions

Question 6

You are studying DNA replication in human cells. You discover a mutant cell line that can carry out DNA replication,but the final product contains both RNA and DNA fragments. What enzyme is likely to be missing in this cell line?

Accepted Answer

A)  flap endonuclease 
B)  DNA ligase 
C)  DNA polymerase I 
D)  DNA helicase 
A)  flap endonuclease 
B)  DNA ligase 
C)  DNA polymerase I 
D)  DNA helicase

Question 7

DNA polymerase III carries out leading and lagging strand synthesis in prokaryotes. How many enzymes accomplish this task in eukaryotes (not counting primer creation)?

Accepted Answer

A)  one 
B)  two 
C)  three 
D)  four 
A)  one 
B)  two 
C)  three 
D)  four

Question 8

The problem of replicating the 3′ ends of chromosomes is solved by using the enzyme telomerase.

Accepted Answer

A) True 
 B)False

Question 9

The movement of the replication fork in bacterial replication is unidirectional.

Accepted Answer

A) True 
 B)False

Question 10

What enzyme relaxes supercoiling ahead of the replication fork?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 11

In E.coli,which protein(s)are necessary for the cell to recognize double-stranded breaks and conduct recombination during meiosis?

Accepted Answer

A)  RecG 
B)  RecA 
C)  RecBCD 
D)  RuvABC 
A)  RecG 
B)  RecA 
C)  RecBCD 
D)  RuvABC

Question 12

Select the statements that are true regarding the initiation of DNA replication in bacteria.

Accepted Answer

A)  It involves a region of the DNA called oriC. 
B)  Proteins bind to the DNA to begin separation of the strands. 
C)  The strands are initially separated at GC-rich regions of DNA. 
D)  Following initial separation, DNA helicase enzymes continue to separate the strands. 
A)  It involves a region of the DNA called oriC. 
B)  Proteins bind to the DNA to begin separation of the strands. 
C)  The strands are initially separated at GC-rich regions of DNA. 
D)  Following initial separation, DNA helicase enzymes continue to separate the strands.

Question 13

In the attachment of a nucleotide to a DNA strand,the cleavage of the dNTP into deoxyribonucleoside monophosphate and pyrophosphate is ________.

Accepted Answer

A)  exergonic 
B)  endergonic 
C)  energy neutral 
A)  exergonic 
B)  endergonic 
C)  energy neutral

Question 14

Replication usually begins in GC-rich regions due to the presence of only 2 hydrogen bonds between the bases.

Accepted Answer

A) True 
 B)False

Question 15

DNA polymerases are unable to replicate what areas of the chromosome?

Accepted Answer

A)  centromeres 
B)  3′ ends 
C)  origins of replication 
D)  5′ ends 
A)  centromeres 
B)  3′ ends 
C)  origins of replication 
D)  5′ ends

Question 16

DNA polymerases add new nucleotides in what direction?

Accepted Answer

A)  5′ to 3′ 
B)  3′ to 5′ 
C)  both directions 
A)  5′ to 3′ 
B)  3′ to 5′ 
C)  both directions

Question 17

Which enzyme is responsible for the majority of DNA synthesis during replication?

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 18

After the action of the helicase,single-stranded binding proteins keep the parental DNA strands from reforming a double helix.

Accepted Answer

A) True 
 B)False

Question 19

A heteroduplex is a region within a DNA double helix that contains base mismatches that can lead to gene conversion.

Accepted Answer

A) True 
 B)False

Question 20

DNA polymerase III has an error in replication once every 100 million nucleotides.

Accepted Answer

A) True 
 B)False

Exam 13: Dna Replication and Recombination

Which of the following best describes the function of the RecA protein in E.coli?

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Homologous recombination that leads to genetic diversity occurs during meiosis II.

Recombination between sister chromatids produces new combinations of alleles that may be beneficial to the species.

The proofreading of the DNA occurs in the ________.

You are studying DNA replication in human cells. You discover a mutant cell line that can carry out DNA replication,but the final product contains both RNA and DNA fragments. What enzyme is likely to be missing in this cell line?

DNA polymerase III carries out leading and lagging strand synthesis in prokaryotes. How many enzymes accomplish this task in eukaryotes (not counting primer creation)?

The problem of replicating the 3′ ends of chromosomes is solved by using the enzyme telomerase.

The movement of the replication fork in bacterial replication is unidirectional.

What enzyme relaxes supercoiling ahead of the replication fork?

In E.coli,which protein(s)are necessary for the cell to recognize double-stranded breaks and conduct recombination during meiosis?

Select the statements that are true regarding the initiation of DNA replication in bacteria.

In the attachment of a nucleotide to a DNA strand,the cleavage of the dNTP into deoxyribonucleoside monophosphate and pyrophosphate is ________.

Replication usually begins in GC-rich regions due to the presence of only 2 hydrogen bonds between the bases.

DNA polymerases are unable to replicate what areas of the chromosome?

DNA polymerases add new nucleotides in what direction?

Which enzyme is responsible for the majority of DNA synthesis during replication?

After the action of the helicase,single-stranded binding proteins keep the parental DNA strands from reforming a double helix.

A heteroduplex is a region within a DNA double helix that contains base mismatches that can lead to gene conversion.

DNA polymerase III has an error in replication once every 100 million nucleotides.

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Molecular Technologies

Genomics

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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