Question 1

If an organism is missing the U5 snRNP,it will have trouble performing which step of splicing?

Accepted Answer

A)  Binding of snRNPs to the 5' splice site 
B)  Binding of snRNPs to the branch site 
C)  Formation of the intron loop 
D)  Exon degredation 
A)  Binding of snRNPs to the 5' splice site 
B)  Binding of snRNPs to the branch site 
C)  Formation of the intron loop 
D)  Exon degredation

Question 2

What RNA modification is important for mRNA stability?

Accepted Answer

A)  alternative splicing 
B)  RNA editing 
C)  base modification 
D)  3' polyA tailing 
E)  processing 
A)  alternative splicing 
B)  RNA editing 
C)  base modification 
D)  3' polyA tailing 
E)  processing

Question 3

The process of RNA editing can alter the base sequence of the mRNA following transcription.

Accepted Answer

A) True 
 B)False

Question 4

The term ribozyme is given to catalytic RNA molecules.

Accepted Answer

A) True 
 B)False

Question 5

In transcription,the closed complex consists of

Accepted Answer

A)  RNA polymerase. 
B)  transcription factors. 
C)  double-stranded DNA. 
D)  single-stranded DNA. 
A)  RNA polymerase. 
B)  transcription factors. 
C)  double-stranded DNA. 
D)  single-stranded DNA.

Question 6

Select the statement that is true regarding tRNA processing in E.coli.

Accepted Answer

A)  RNaseP acts as an endonuclease. 
B)  The recognition sequence of RNaseD is 3'CCA5'. 
C)  RNaseD acts as an endonuclease. 
D)  The action of RNaseP produces the correct 3' end of the mature tRNA. 
A)  RNaseP acts as an endonuclease. 
B)  The recognition sequence of RNaseD is 3'CCA5'. 
C)  RNaseD acts as an endonuclease. 
D)  The action of RNaseP produces the correct 3' end of the mature tRNA.

Question 7

In eukaryotic organisms,the processing of the 45S rRNA into 5.8S,18S,and 28S rRNA occurs where?

Accepted Answer

A)  in the cytoplasm 
B)  in the nucleolus 
C)  in the endoplasmic reticulum 
D)  in the Golgi body 
E)  throughout the cell 
A)  in the cytoplasm 
B)  in the nucleolus 
C)  in the endoplasmic reticulum 
D)  in the Golgi body 
E)  throughout the cell

Question 8

What structural motif is used by sigma factor to bind to DNA?

Accepted Answer

A)  zinc-finger 
B)  helix-turn-helix 
C)  leucine zipper 
D)  homeodomain 
A)  zinc-finger 
B)  helix-turn-helix 
C)  leucine zipper 
D)  homeodomain

Question 9

What is the name of the transcription start site?

Accepted Answer

A)  promoter 
B)  terminator 
C)  regulation sequences 
D)  transcription factors 
A)  promoter 
B)  terminator 
C)  regulation sequences 
D)  transcription factors

Question 10

How many general transcription factors are needed for eukaryotic translation?

Accepted Answer

A)  3 
B)  4 
C)  5 
D)  6 
A)  3 
B)  4 
C)  5 
D)  6

Question 11

What process is important for the initiation of translation?

Accepted Answer

A)  alternative splicing 
B)  RNA editing 
C)  5' capping 
D)  base modification 
A)  alternative splicing 
B)  RNA editing 
C)  5' capping 
D)  base modification

Question 12

What do both the rho-dependent and rho-independent mechanisms of termination have in common?

Accepted Answer

A)  terminate transcription immediately after the stop codon 
B)  a sequence rich with A-U base pairs 
C)  Both require a helicase to separate the DNA-RNA complex. 
D)  formation of a stem-loop structure 
A)  terminate transcription immediately after the stop codon 
B)  a sequence rich with A-U base pairs 
C)  Both require a helicase to separate the DNA-RNA complex. 
D)  formation of a stem-loop structure

Question 13

You are studying transcription in two different unknown systems. Select the experimental questions you could test that would allow you to definitively determine if the system is eukaryotic or prokaryotic.

Accepted Answer

A)  Does transcription in the system require a promoter? 
B)  Does RNA capping occur? 
C)  Does RNA splicing occur, and if so is it self-splicing? 
D)  Does RNA tailing occur? 
E)  How many RNA polymerases are present? 
A)  Does transcription in the system require a promoter? 
B)  Does RNA capping occur? 
C)  Does RNA splicing occur, and if so is it self-splicing? 
D)  Does RNA tailing occur? 
E)  How many RNA polymerases are present?

Question 14

What general transcription factor is a helicase?

Accepted Answer

A)  TFIID 
B)  TFIIH 
C)  TFIIF 
D)  TFIIB 
A)  TFIID 
B)  TFIIH 
C)  TFIIF 
D)  TFIIB

Question 15

Select the eukaryotic regulatory elements.

Accepted Answer

A)  enhancers 
B)  silencers 
C)  cis-acting elements 
D)  Pribnow box 
A)  enhancers 
B)  silencers 
C)  cis-acting elements 
D)  Pribnow box

Question 16

Which modifications are typically found on eukaryotic mRNAs

Accepted Answer

A)  Capping only 
B)  Addition of poly A only 
C)  Capping and poly-A addition 
D)  Eukayotic mRNAs are not modified before translation 
A)  Capping only 
B)  Addition of poly A only 
C)  Capping and poly-A addition 
D)  Eukayotic mRNAs are not modified before translation

Question 17

Most eukaryotic genes are colinear.

Accepted Answer

A)  True 
B)  False 
C)  It is unknown at this time. 
A)  True 
B)  False 
C)  It is unknown at this time.

Question 18

The unit of transcription at the molecular level is the gene.

Accepted Answer

A) True 
 B)False

Question 19

What protein allows RNA polymerase II to interact with transcription factors bound to enhancers or silencers?

Accepted Answer

A)  mediator 
B)  TFIIB 
C)  TFIID 
D)  sigma factors 
A)  mediator 
B)  TFIIB 
C)  TFIID 
D)  sigma factors

Question 20

Sigma factor belongs to a general group of proteins called the transcription factors.

Accepted Answer

A) True 
 B)False

Exam 14: Gene Transcription and RNA Modification

If an organism is missing the U5 snRNP,it will have trouble performing which step of splicing?

What RNA modification is important for mRNA stability?

The process of RNA editing can alter the base sequence of the mRNA following transcription.

The term ribozyme is given to catalytic RNA molecules.

In transcription,the closed complex consists of

Select the statement that is true regarding tRNA processing in E.coli.

In eukaryotic organisms,the processing of the 45S rRNA into 5.8S,18S,and 28S rRNA occurs where?

What structural motif is used by sigma factor to bind to DNA?

What is the name of the transcription start site?

How many general transcription factors are needed for eukaryotic translation?

What process is important for the initiation of translation?

What do both the rho-dependent and rho-independent mechanisms of termination have in common?

You are studying transcription in two different unknown systems. Select the experimental questions you could test that would allow you to definitively determine if the system is eukaryotic or prokaryotic.

What general transcription factor is a helicase?

Select the eukaryotic regulatory elements.

Which modifications are typically found on eukaryotic mRNAs

Most eukaryotic genes are colinear.

The unit of transcription at the molecular level is the gene.

What protein allows RNA polymerase II to interact with transcription factors bound to enhancers or silencers?

Sigma factor belongs to a general group of proteins called the transcription factors.

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Dna Replication and Recombination

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Molecular Technologies

Genomics

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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