Question 1

Which of these would not be used to introduce DNA in animal cells?

Accepted Answer

A)  liposomes 
B)  electroporation 
C)  microinjection 
D)  particle bombardment 
E)  a Ti plasmid 
A)  liposomes 
B)  electroporation 
C)  microinjection 
D)  particle bombardment 
E)  a Ti plasmid E

Question 2

Genetic modification

Accepted Answer

A)  alters the genetic codes so that one species' code is like another's. 
B)  alters, deletes or adds sugars and phosphates in a nucleus. 
C)  alters, deletes, or adds DNA to a cell. 
D)  removes RNA from a cell. 
E)  substitutes entire nuclei to genetically engineer a cell. 
A)  alters the genetic codes so that one species' code is like another's. 
B)  alters, deletes or adds sugars and phosphates in a nucleus. 
C)  alters, deletes, or adds DNA to a cell. 
D)  removes RNA from a cell. 
E)  substitutes entire nuclei to genetically engineer a cell. C

Question 3

Proteins isolated from bacteria and used in recombinant DNA technology to cut DNA at specific sequences are

Accepted Answer

A)  restriction enzymes. 
B)  bacteriophage enzymes. 
C)  plasmids. 
D)  methylating enzymes. 
E)  telomerases. 
A)  restriction enzymes. 
B)  bacteriophage enzymes. 
C)  plasmids. 
D)  methylating enzymes. 
E)  telomerases. A

Question 4

Which technique uses tiny needles to deliver DNA into animal cells?

Accepted Answer

A)  electrophoresis 
B)  microinjection 
C)  particle bombardment 
D)  electroporation 
E)  bacteriophage bombardment 
A)  electrophoresis 
B)  microinjection 
C)  particle bombardment 
D)  electroporation 
E)  bacteriophage bombardment

Question 5

The first patent on a living organism was granted in

Accepted Answer

A)  1790, when the U.S. passed patent law. 
B)  1873, for Louis Pasteur's use of yeast. 
C)  1900, when Mendel's laws were rediscovered. 
D)  1953, when Watson and Crick discovered the structure of DNA. 
E)  1978, when modern biotechnology began with recombinant insulin. 
A)  1790, when the U.S. passed patent law. 
B)  1873, for Louis Pasteur's use of yeast. 
C)  1900, when Mendel's laws were rediscovered. 
D)  1953, when Watson and Crick discovered the structure of DNA. 
E)  1978, when modern biotechnology began with recombinant insulin.

Question 6

The type of RNA that carries out RNA intereference is

Accepted Answer

A)  tRNA. 
B)  mRNA. 
C)  rRNA. 
D)  siRNA. 
E)  fRNA. 
A)  tRNA. 
B)  mRNA. 
C)  rRNA. 
D)  siRNA. 
E)  fRNA.

Question 7

To create a transgenic organism,the researcher

Accepted Answer

A)  introduces foreign DNA into a gamete or fertilized ovum. 
B)  injects a gene of interest into a somatic cell. 
C)  injects a gene of interest into several somatic cells. 
D)  introduces foreign DNA into somatic cells in culture and transplants them. 
E)  uses site directed mutagenesis on the adult. 
A)  introduces foreign DNA into a gamete or fertilized ovum. 
B)  injects a gene of interest into a somatic cell. 
C)  injects a gene of interest into several somatic cells. 
D)  introduces foreign DNA into somatic cells in culture and transplants them. 
E)  uses site directed mutagenesis on the adult.

Question 8

Automated PCR machines use a heat stable

Accepted Answer

A)  DNA ligase. 
B)  helicase. 
C)  primase. 
D)  DNA polymerase. 
E)  amino acid synthetase. 
A)  DNA ligase. 
B)  helicase. 
C)  primase. 
D)  DNA polymerase. 
E)  amino acid synthetase.

Question 9

What innovation has improved on the thermal cycler in speeding PCR?

Accepted Answer

A)  layered silicon 
B)  layered DNA 
C)  a new algorithm 
D)  using DNA polymerase from a cheetah 
E)  alternating DNA and RNA polymerase 
A)  layered silicon 
B)  layered DNA 
C)  a new algorithm 
D)  using DNA polymerase from a cheetah 
E)  alternating DNA and RNA polymerase

Question 10

A cDNA library represents

Accepted Answer

A)  all the DNA that comprises a genome. 
B)  all the possible DNA codons used to specify amino acids. 
C)  all the books in a science library that mention DNA. 
D)  all the protein-encoding genes in a genome. 
E)  all the non protein-encoding DNA sequences of a genome. 
A)  all the DNA that comprises a genome. 
B)  all the possible DNA codons used to specify amino acids. 
C)  all the books in a science library that mention DNA. 
D)  all the protein-encoding genes in a genome. 
E)  all the non protein-encoding DNA sequences of a genome.

Question 11

Patent law as it pertains to biotechnology has had to change in recent years in response to

Accepted Answer

A)  the changeable nature of the genetic code. 
B)  the number of people asking for genetic tests. 
C)  the increasing number of human genes. 
D)  the greatly accelerated speed of DNA sequencing. 
E)  the fact that not as many lawyers are going into patent law. 
A)  the changeable nature of the genetic code. 
B)  the number of people asking for genetic tests. 
C)  the increasing number of human genes. 
D)  the greatly accelerated speed of DNA sequencing. 
E)  the fact that not as many lawyers are going into patent law.

Question 12

The field of biotechnology began

Accepted Answer

A)  in 2001, when the human genome was sequenced. 
B)  in 1961, when the genetic code was deciphered. 
C)  in 1953, when the structure of DNA was discovered. 
D)  in the early 1900s, when Mendel's laws were rediscovered. 
E)  with the advent of agriculture, about 10,000 years ago. 
A)  in 2001, when the human genome was sequenced. 
B)  in 1961, when the genetic code was deciphered. 
C)  in 1953, when the structure of DNA was discovered. 
D)  in the early 1900s, when Mendel's laws were rediscovered. 
E)  with the advent of agriculture, about 10,000 years ago.

Question 13

Morpholinos are

Accepted Answer

A)  short DNA sequences found only in populations that trace their roots to Italy. 
B)  short synthetic molecules similar to DNA that are complementary to splice site mutations. 
C)  used in DNA amplification. 
D)  types of DNA microarrays used to study gene expression. 
E)  a type of recombinant bacteria. 
A)  short DNA sequences found only in populations that trace their roots to Italy. 
B)  short synthetic molecules similar to DNA that are complementary to splice site mutations. 
C)  used in DNA amplification. 
D)  types of DNA microarrays used to study gene expression. 
E)  a type of recombinant bacteria.

Question 14

Participants in RNAi include

Accepted Answer

A)  dicer, RISK, and argonaute. 
B)  electroporation, liposomes, and particle bombardment. 
C)  tRNA, mRNA, and rRNA. 
D)  RNA, DNA, and protein. 
E)  DNA polymerase, ligase, and acetylase. 
A)  dicer, RISK, and argonaute. 
B)  electroporation, liposomes, and particle bombardment. 
C)  tRNA, mRNA, and rRNA. 
D)  RNA, DNA, and protein. 
E)  DNA polymerase, ligase, and acetylase.

Question 15

A drug produced using recombinant DNA technology that is used illegally by athletes is

Accepted Answer

A)  insulin. 
B)  streptokinase. 
C)  tPA. 
D)  EPO. 
E)  telomerase. 
A)  insulin. 
B)  streptokinase. 
C)  tPA. 
D)  EPO. 
E)  telomerase.

Question 16

Transgenic pharming uses _______ to produce recombinant proteins in _______.

Accepted Answer

A)  cows; milk 
B)  bacteriophage; bacteria 
C)  bacteria; culture media 
D)  fungi; culture media 
E)  viruses; cell culture 
A)  cows; milk 
B)  bacteriophage; bacteria 
C)  bacteria; culture media 
D)  fungi; culture media 
E)  viruses; cell culture

Question 17

Tiny fat bubbles used to deliver genes are

Accepted Answer

A)  electropores. 
B)  phospholipids. 
C)  cholesterols. 
D)  liposomes. 
E)  plasmids. 
A)  electropores. 
B)  phospholipids. 
C)  cholesterols. 
D)  liposomes. 
E)  plasmids.

Question 18

A limitation of transgenesis is that

Accepted Answer

A)  it only works on very short genes. 
B)  it only works on vertebrates. 
C)  the transgene can insert anywhere in the genome. 
D)  the transgene could disappear before it inserts in the genome. 
E)  it only works within species. 
A)  it only works on very short genes. 
B)  it only works on vertebrates. 
C)  the transgene can insert anywhere in the genome. 
D)  the transgene could disappear before it inserts in the genome. 
E)  it only works within species.

Question 19

In 1975,scientists convened in Asilomar,California and

Accepted Answer

A)  determined that restriction enzymes could cut DNA. 
B)  created the first transgenic animals. 
C)  reviewed the use of drugs produced by recombinant DNA technology. 
D)  drew up guidelines to regulate recombinant DNA technology. 
E)  developed PCR for amplifying DNA. 
A)  determined that restriction enzymes could cut DNA. 
B)  created the first transgenic animals. 
C)  reviewed the use of drugs produced by recombinant DNA technology. 
D)  drew up guidelines to regulate recombinant DNA technology. 
E)  developed PCR for amplifying DNA.

Question 20

The term "patent thicket" refers to

Accepted Answer

A)  genetic modification of trees. 
B)  the fact that many people are requesting genetic tests from their doctors as well as taking direct-to-consumer tests on the Web. 
C)  the backlog at the patent office. 
D)  the requirement for companies that offer genetic test panels to license every piece of DNA or gene that they put into kits. 
E)  the fact that the DNA sequence, exon pattern, SNP pattern, or gene expression pattern of a gene can be patented separately. 
A)  genetic modification of trees. 
B)  the fact that many people are requesting genetic tests from their doctors as well as taking direct-to-consumer tests on the Web. 
C)  the backlog at the patent office. 
D)  the requirement for companies that offer genetic test panels to license every piece of DNA or gene that they put into kits. 
E)  the fact that the DNA sequence, exon pattern, SNP pattern, or gene expression pattern of a gene can be patented separately.

Exam 19: Genetic Technologies: Amplifying, Modifying, and Monitoring DNA

Which of these would not be used to introduce DNA in animal cells?

Genetic modification

Proteins isolated from bacteria and used in recombinant DNA technology to cut DNA at specific sequences are

Which technique uses tiny needles to deliver DNA into animal cells?

The first patent on a living organism was granted in

The type of RNA that carries out RNA intereference is

To create a transgenic organism,the researcher

Automated PCR machines use a heat stable

What innovation has improved on the thermal cycler in speeding PCR?

A cDNA library represents

Patent law as it pertains to biotechnology has had to change in recent years in response to

The field of biotechnology began

Morpholinos are

Participants in RNAi include

A drug produced using recombinant DNA technology that is used illegally by athletes is

Transgenic pharming uses _______ to produce recombinant proteins in _______.

Tiny fat bubbles used to deliver genes are

A limitation of transgenesis is that

In 1975,scientists convened in Asilomar,California and

The term "patent thicket" refers to

Overview of Genetics

Cells

Meiosis and Development

Single-Gene Inheritance

Beyond Mendels Law

Matters of Sex

Multifactorial Traits

Genetics of Behavior

DNA Structure and Replication

Gene Action: From Dna to Protein

Gene Expression and Epigenetics

Gene Mutation

Chromosomes

Constant Allele Frequencies

Changing Allele Frequencies

Human Ancestry

Genetics of Immunity

Genetics of Cancer

Genetic Testing and Treatment

Reproductive Technologies

Genomics

Filters

Transgenic pharming uses _ to produce recombinant proteins in _.