Question 1

Transgenic plants must be approved by the Food and Drug Administration before they are considered safe for human consumption.

Accepted Answer

A) True 
 B)False  True

Question 2

What was the minimal requirement to perform a "classic DNA fingerprinting" test in order to associate a blood sample with a criminal?

Accepted Answer

A) one sample of DNA,PCR amplification,restriction enzymes,and gel electrophoresis 
B) one sample of DNA,restriction enzymes,a DNA synthesizer,and gel electrophoresis 
C) two samples of DNA,PCR amplification,restriction enzymes,and gel electrophoresis 
D) two samples of DNA,PCR amplification,a DNA synthesizer,and gel electrophoresis 
E) two samples of DNA,PCR amplification,restriction enzymes,and a DNA synthesizer 
A) one sample of DNA,PCR amplification,restriction enzymes,and gel electrophoresis 
B) one sample of DNA,restriction enzymes,a DNA synthesizer,and gel electrophoresis 
C) two samples of DNA,PCR amplification,restriction enzymes,and gel electrophoresis 
D) two samples of DNA,PCR amplification,a DNA synthesizer,and gel electrophoresis 
E) two samples of DNA,PCR amplification,restriction enzymes,and a DNA synthesizer C

Question 3

What is the major reason why xenotransplantation is not being widely used in medicine today?

Accepted Answer

A) The human patient will often reject the animal organ. 
B) There are not enough animal organs available for transplant. 
C) People are not willing to accept an animal organ during a transplant. 
D) There are not enough human organs to be able to transplant them to the animals in need. 
A) The human patient will often reject the animal organ. 
B) There are not enough animal organs available for transplant. 
C) People are not willing to accept an animal organ during a transplant. 
D) There are not enough human organs to be able to transplant them to the animals in need. A

Question 4

All of the following statements are true about restriction enzymes EXCEPT

Accepted Answer

A) they are made by bacteria and viruses. 
B) hundreds of different ones have been isolated and purified. 
C) they produce single-stranded complementary ends that can join to two different DNA strands by complementary base pairing. 
D) each enzyme cuts DNA at a different specific base sequence. 
A) they are made by bacteria and viruses. 
B) hundreds of different ones have been isolated and purified. 
C) they produce single-stranded complementary ends that can join to two different DNA strands by complementary base pairing. 
D) each enzyme cuts DNA at a different specific base sequence.

Question 5

To carry out a polymerase chain reaction (PCR),you must have DNA polymerase and

Accepted Answer

A) a blueprint or gene map of the sequence you wish to copy. 
B) the nucleotides to synthesize new DNA strands. 
C) a DNA synthesizer machine. 
D) a DNA probe. 
A) a blueprint or gene map of the sequence you wish to copy. 
B) the nucleotides to synthesize new DNA strands. 
C) a DNA synthesizer machine. 
D) a DNA probe.

Question 6

Human gene therapy

Accepted Answer

A) includes the insertion of genetic material into human cells for the treatment of a genetic disease. 
B) has been used for treatment of children who have severe combined immunodeficiency. 
C) has been used in a trial to treat familial hypercholesterolemia. 
D) All of the choices are correct. 
A) includes the insertion of genetic material into human cells for the treatment of a genetic disease. 
B) has been used for treatment of children who have severe combined immunodeficiency. 
C) has been used in a trial to treat familial hypercholesterolemia. 
D) All of the choices are correct.

Question 7

Familial hypercholesterolemia

Accepted Answer

A) is characterized by high blood levels of cholesterol. 
B) is a condition that develops when liver cells lack a receptor protein for removing cholesterol from the blood. 
C) may be treated by ex vivo gene therapy. 
D) All of the above are true. 
E) Only two of the above are true. 
A) is characterized by high blood levels of cholesterol. 
B) is a condition that develops when liver cells lack a receptor protein for removing cholesterol from the blood. 
C) may be treated by ex vivo gene therapy. 
D) All of the above are true. 
E) Only two of the above are true.

Question 8

Biotechnology products produced by bacteria include all of the following EXCEPT

Accepted Answer

A) an antibody to deliver radioisotopes to tumor cells. 
B) clotting factor VIII. 
C) human growth hormone. 
D) insulin. 
A) an antibody to deliver radioisotopes to tumor cells. 
B) clotting factor VIII. 
C) human growth hormone. 
D) insulin.

Question 9

All of the following statements about CNVs are true EXCEPT

Accepted Answer

A) CNVs occur when genes have changed their number. 
B) the changes may result from fork stalling and template switching. 
C) CNVs have known links to disease. 
D) All of the above are true. 
A) CNVs occur when genes have changed their number. 
B) the changes may result from fork stalling and template switching. 
C) CNVs have known links to disease. 
D) All of the above are true.

Question 10

What enzyme is used to make cDNA from mRNA?

Accepted Answer

A) DNA ligase 
B) helicase 
C) reverse transcriptase 
D) RNA polymerase 
E) restriction enzyme 
A) DNA ligase 
B) helicase 
C) reverse transcriptase 
D) RNA polymerase 
E) restriction enzyme

Question 11

What consequences could arise if the field of bioinformatics ceased to exist as a field of study?

Accepted Answer

A) Progress in determining the function of DNA sequences,making comparisons between our genome and other model organisms,and understanding how genes and proteins interact with cells would stop. 
B) Many cures for genetic disorders would not be discovered. 
C) The creation of new transgenic plants,animals and bacteria that benefit society would not occur. 
D) All of these are consequences if the field of bioinformatics ceased to exist as a field of study. 
A) Progress in determining the function of DNA sequences,making comparisons between our genome and other model organisms,and understanding how genes and proteins interact with cells would stop. 
B) Many cures for genetic disorders would not be discovered. 
C) The creation of new transgenic plants,animals and bacteria that benefit society would not occur. 
D) All of these are consequences if the field of bioinformatics ceased to exist as a field of study.

Question 12

Gene pharming is the use of

Accepted Answer

A) transgenic plants to produce larger vegetables and sweeter fruits. 
B) bacteria to produce organic compounds like aspartame and phenylalanine. 
C) STR profiling to determine evolutionary relationships among organisms. 
D) transgenic farm animals to produce pharmaceuticals. 
A) transgenic plants to produce larger vegetables and sweeter fruits. 
B) bacteria to produce organic compounds like aspartame and phenylalanine. 
C) STR profiling to determine evolutionary relationships among organisms. 
D) transgenic farm animals to produce pharmaceuticals.

Question 13

With both bacterial and eukaryotic cells constantly dying and breaking down around us,and with a tremendous load of fragmentary DNA washing around in the fluid world,why aren't genes being transferred among various living organisms at a massive scale daily?

Accepted Answer

A) DNA that is not protected inside a protein coat or membrane is very vulnerable. 
B) Living cells maintain a very effective barrier to the introduction of large molecules. 
C) Since a vector appears necessary to snatch up DNA from one protected source and inject it into a cell,"naked" DNA is apparently vulnerable and unable to cross membranes easily. 
D) All of the choices are correct. 
E) None of the choices are correct,since DNA is readily exchanged among all organisms in nature. 
A) DNA that is not protected inside a protein coat or membrane is very vulnerable. 
B) Living cells maintain a very effective barrier to the introduction of large molecules. 
C) Since a vector appears necessary to snatch up DNA from one protected source and inject it into a cell,"naked" DNA is apparently vulnerable and unable to cross membranes easily. 
D) All of the choices are correct. 
E) None of the choices are correct,since DNA is readily exchanged among all organisms in nature.

Question 14

A common method used to introduce rDNA into bacterial host cells is

Accepted Answer

A) viral infection. 
B) bacteriophage infection. 
C) plasmid transfer. 
D) microinjection of DNA. 
E) laser irradiation of cells in a fluid containing DNA. 
A) viral infection. 
B) bacteriophage infection. 
C) plasmid transfer. 
D) microinjection of DNA. 
E) laser irradiation of cells in a fluid containing DNA.

Question 15

Transgenic _______ have been given suicide genes that cause them to self-destruct when the job for which they are engineered has been accomplished.

Accepted Answer

A) animals 
B) bacteria 
C) plants 
D) fungi 
E) All of the choices are correct. 
A) animals 
B) bacteria 
C) plants 
D) fungi 
E) All of the choices are correct.

Question 16

The polymerase chain reaction uses RNA polymerase.

Accepted Answer

A) True 
 B)False

Question 17

Proteomics involves the application of computer technologies to study databases of genetic information.

Accepted Answer

A) True 
 B)False

Question 18

Transgenic plant products include all of the following EXCEPT

Accepted Answer

A) soybeans that are resistant to a common herbicide. 
B) an antibody produced by soybeans to treat genital herpes. 
C) suicide genes that cause the plants to self destruct after their product has been produced. 
D) antibodies produced by corn plants that deliver radioisotopes to tumor cells. 
A) soybeans that are resistant to a common herbicide. 
B) an antibody produced by soybeans to treat genital herpes. 
C) suicide genes that cause the plants to self destruct after their product has been produced. 
D) antibodies produced by corn plants that deliver radioisotopes to tumor cells.

Question 19

Which of the following is mismatched?

Accepted Answer

A) bioinformatics - the study of a genomic and proteomic information using computer analysis 
B) polymerase chain reaction - process that separates DNA fragments according to size 
C) genomics - the study of the genomes of humans and other organisms 
D) proteomics - the study of species' proteins 
A) bioinformatics - the study of a genomic and proteomic information using computer analysis 
B) polymerase chain reaction - process that separates DNA fragments according to size 
C) genomics - the study of the genomes of humans and other organisms 
D) proteomics - the study of species' proteins

Question 20

Under which of the following circumstances would a DNA microarray be useful?

Accepted Answer

A) It would enable a researcher to determine the differences in gene expression between cell types. 
B) Researchers are trying to determine if an individual has inherited a genetic disease from their parents. 
C) It would enable doctors to determine which genes are responsible for producing diseased tissue in an individual. 
D) All of these are circumstances in which a DNA microarray would be useful. 
E) None of these are circumstances in which a DNA microarray would be useful. 
A) It would enable a researcher to determine the differences in gene expression between cell types. 
B) Researchers are trying to determine if an individual has inherited a genetic disease from their parents. 
C) It would enable doctors to determine which genes are responsible for producing diseased tissue in an individual. 
D) All of these are circumstances in which a DNA microarray would be useful. 
E) None of these are circumstances in which a DNA microarray would be useful.

Exam 14: Biotechnology and Genomics

Transgenic plants must be approved by the Food and Drug Administration before they are considered safe for human consumption.

What was the minimal requirement to perform a "classic DNA fingerprinting" test in order to associate a blood sample with a criminal?

What is the major reason why xenotransplantation is not being widely used in medicine today?

All of the following statements are true about restriction enzymes EXCEPT

To carry out a polymerase chain reaction (PCR),you must have DNA polymerase and

Human gene therapy

Familial hypercholesterolemia

Biotechnology products produced by bacteria include all of the following EXCEPT

All of the following statements about CNVs are true EXCEPT

What enzyme is used to make cDNA from mRNA?

What consequences could arise if the field of bioinformatics ceased to exist as a field of study?

Gene pharming is the use of

With both bacterial and eukaryotic cells constantly dying and breaking down around us,and with a tremendous load of fragmentary DNA washing around in the fluid world,why aren't genes being transferred among various living organisms at a massive scale daily?

A common method used to introduce rDNA into bacterial host cells is

Transgenic _______ have been given suicide genes that cause them to self-destruct when the job for which they are engineered has been accomplished.

The polymerase chain reaction uses RNA polymerase.

Proteomics involves the application of computer technologies to study databases of genetic information.

Transgenic plant products include all of the following EXCEPT

Which of the following is mismatched?

Under which of the following circumstances would a DNA microarray be useful?

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Basic Chemistry

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Molecular Biology of the Gene

Regulation of Gene Expression

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How Populations Evolve

Speciation and Macroevolution

Origin and History of Life

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Protist Evolution and Diversity

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