Question 1

Gene therapy in humans has been used to treat

Accepted Answer

A) cystic fibrosis. 
B) cancer. 
C) inborn errors of metabolism. 
D) All of the above. 
E) Only two of the above. 
A) cystic fibrosis. 
B) cancer. 
C) inborn errors of metabolism. 
D) All of the above. 
E) Only two of the above.

Question 2

All of the following are true regarding transgenic animals EXCEPT

Accepted Answer

A) foreign genes are micro-injected into eggs,fertilized in vitro,and the zygotes are placed in host females to develop. 
B) the process has inserted bovine growth hormone into fish,cows,and other animals. 
C) the process has been used to develop organisms that are partially animals and partially plant. 
D) the product desired by the transgenic procedure may be secreted in the milk of female offspring. 
A) foreign genes are micro-injected into eggs,fertilized in vitro,and the zygotes are placed in host females to develop. 
B) the process has inserted bovine growth hormone into fish,cows,and other animals. 
C) the process has been used to develop organisms that are partially animals and partially plant. 
D) the product desired by the transgenic procedure may be secreted in the milk of female offspring.

Question 3

Gene therapy is being used in cancer patients to make healthy cells more tolerant of chemotherapy and to make tumors more vulnerable to chemotherapy.

Accepted Answer

A) True 
 B)False

Question 4

Plants are being genetically engineered to have

Accepted Answer

A) a requirement for more fertilizer. 
B) an increased water requirement. 
C) the ability to produce human proteins. 
D) increased susceptibility to herbicides. 
E) All of the choices are correct. 
A) a requirement for more fertilizer. 
B) an increased water requirement. 
C) the ability to produce human proteins. 
D) increased susceptibility to herbicides. 
E) All of the choices are correct.

Question 5

Which of the following molecules forms lengths of DNA with "sticky ends"?

Accepted Answer

A) DNA ligase 
B) DNA polymerase 
C) RNA polymerase 
D) reverse transcriptase 
E) restriction enzyme 
A) DNA ligase 
B) DNA polymerase 
C) RNA polymerase 
D) reverse transcriptase 
E) restriction enzyme

Question 6

Place the following steps in cloning DNA in the correct order 1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product

Accepted Answer

A) 1,2,3,4,5 
B) 5,3,2,4,1 
C) 2,5,3,1,4 
D) 3,2,4,1,5 
A) 1,2,3,4,5 
B) 5,3,2,4,1 
C) 2,5,3,1,4 
D) 3,2,4,1,5

Question 7

When bone marrow stem cells are removed from the patient,infected with a virus that carries a normal gene,and returned to the patient,this is an example of

Accepted Answer

A) PCR. 
B) chemotherapy. 
C) viral disinfection. 
D) in vivo gene therapy. 
E) ex vivo gene therapy. 
A) PCR. 
B) chemotherapy. 
C) viral disinfection. 
D) in vivo gene therapy. 
E) ex vivo gene therapy.

Question 8

The function of DNA ligase in recombinant technology is to

Accepted Answer

A) cut DNA into many fragments. 
B) carry DNA into a new cell. 
C) seal DNA into an opening created by restriction enzymes. 
D) make millions of copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges. 
A) cut DNA into many fragments. 
B) carry DNA into a new cell. 
C) seal DNA into an opening created by restriction enzymes. 
D) make millions of copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges.

Question 9

The function of a vector in genetic engineering is to

Accepted Answer

A) cut DNA into many fragments. 
B) introduce rDNA into a host cell. 
C) link together newly joined fragments of DNA. 
D) make millions of copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges. 
A) cut DNA into many fragments. 
B) introduce rDNA into a host cell. 
C) link together newly joined fragments of DNA. 
D) make millions of copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges.

Question 10

How are DNA microarrays used in the study of genomics?

Accepted Answer

A) They are used to identify the mutations that an individual possesses. 
B) They can be used to determine which genes are turned on or off in an individual. 
C) They can be used to help determine an individuals genetic profile. 
D) All of these are uses for DNA microarrays. 
E) None of these are uses for DNA microarrays. 
A) They are used to identify the mutations that an individual possesses. 
B) They can be used to determine which genes are turned on or off in an individual. 
C) They can be used to help determine an individuals genetic profile. 
D) All of these are uses for DNA microarrays. 
E) None of these are uses for DNA microarrays.

Question 11

Which of the following health disorders can be treated with ex vivo gene therapy?

Accepted Answer

A) Liver cells lack a protein that enables it to remove cholesterol from the blood which leads to high levels of cholesterol in the blood.This can increase the patients susceptibility to heart attacks. 
B) The liver cells lack a protein that causes it to produce excessive cholesterol which leads to high levels of cholesterol in the blood.This can increase the patients susceptibility to heart attacks. 
C) A gene is implanted that will code for the correct transmembrane carrier of the chloride ion. 
D) Genes are implanted that make healthy cells more tolerant to chemotherapy while making the tumor cells more susceptible to chemotherapy 
A) Liver cells lack a protein that enables it to remove cholesterol from the blood which leads to high levels of cholesterol in the blood.This can increase the patients susceptibility to heart attacks. 
B) The liver cells lack a protein that causes it to produce excessive cholesterol which leads to high levels of cholesterol in the blood.This can increase the patients susceptibility to heart attacks. 
C) A gene is implanted that will code for the correct transmembrane carrier of the chloride ion. 
D) Genes are implanted that make healthy cells more tolerant to chemotherapy while making the tumor cells more susceptible to chemotherapy

Question 12

Gene cloning can be accomplished by recombinant DNA technology and polymerase chain reactions.

Accepted Answer

A) True 
 B)False

Question 13

What is the function of gel electrophoresis in genetic engineering?

Accepted Answer

A) cut DNA into many fragments 
B) carry DNA into a new cell 
C) link together newly joined fragments of DNA 
D) make millions of copies of a specific segment of DNA 
E) separate fragments of DNA by their length and electrical charges 
A) cut DNA into many fragments 
B) carry DNA into a new cell 
C) link together newly joined fragments of DNA 
D) make millions of copies of a specific segment of DNA 
E) separate fragments of DNA by their length and electrical charges

Question 14

The function of the polymerase chain reaction in genetic engineering is to

Accepted Answer

A) cut DNA into many fragments. 
B) carry DNA into a new cell. 
C) link together newly joined fragments of DNA. 
D) make multiple copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges. 
A) cut DNA into many fragments. 
B) carry DNA into a new cell. 
C) link together newly joined fragments of DNA. 
D) make multiple copies of a specific segment of DNA. 
E) separate fragments of DNA by their length and electrical charges.

Question 15

Animal cloning is a difficult process with a low success rate.

Accepted Answer

A) True 
 B)False

Question 16

Which description best fits the term xenotransplantation?

Accepted Answer

A) The use of animal organs in human transplant patients. 
B) The use of human organs in animal transplants. 
C) The use of animal DNA during genetic modification. 
D) None of these descriptions fit xenotransplantation. 
A) The use of animal organs in human transplant patients. 
B) The use of human organs in animal transplants. 
C) The use of animal DNA during genetic modification. 
D) None of these descriptions fit xenotransplantation.

Question 17

A transgenic organism is

Accepted Answer

A) one that contains a gene from another organism of the same or different species. 
B) one produced by cloning a mutant cell. 
C) one that acts as the donor for DNA to be moved into another organism. 
D) one produced by the polymerase chain reaction. 
E) any genetically modified organism resulting from laboratory research. 
A) one that contains a gene from another organism of the same or different species. 
B) one produced by cloning a mutant cell. 
C) one that acts as the donor for DNA to be moved into another organism. 
D) one produced by the polymerase chain reaction. 
E) any genetically modified organism resulting from laboratory research.

Question 18

DNA fingerprinting may be used to establish paternity.Analyze the banding pattern to determine the father of the child.

Accepted Answer

A) Male 1 
B) Male 2 
A) Male 1 
B) Male 2

Question 19

It is estimated that humans have approximately ______ base pairs in our genome.

Accepted Answer

A) 3 billion 
B) 2.5 million 
C) 180 million 
D) 12 trillion 
A) 3 billion 
B) 2.5 million 
C) 180 million 
D) 12 trillion

Question 20

What is the first step in producing a transgenic animal?

Accepted Answer

A) implantation of a donor egg into the host mother. 
B) inject the desired gene(s)into a donor egg. 
C) transgenic organism produces offspring that have the desired gene which can then produce more transgenic offspring. 
D) transgenic eggs are harvested from the surrogate mother. 
A) implantation of a donor egg into the host mother. 
B) inject the desired gene(s)into a donor egg. 
C) transgenic organism produces offspring that have the desired gene which can then produce more transgenic offspring. 
D) transgenic eggs are harvested from the surrogate mother.

Exam 14: Biotechnology and Genomics

Gene therapy in humans has been used to treat

All of the following are true regarding transgenic animals EXCEPT

Gene therapy is being used in cancer patients to make healthy cells more tolerant of chemotherapy and to make tumors more vulnerable to chemotherapy.

Plants are being genetically engineered to have

Which of the following molecules forms lengths of DNA with "sticky ends"?

When bone marrow stem cells are removed from the patient,infected with a virus that carries a normal gene,and returned to the patient,this is an example of

The function of DNA ligase in recombinant technology is to

The function of a vector in genetic engineering is to

How are DNA microarrays used in the study of genomics?

Which of the following health disorders can be treated with ex vivo gene therapy?

Gene cloning can be accomplished by recombinant DNA technology and polymerase chain reactions.

What is the function of gel electrophoresis in genetic engineering?

The function of the polymerase chain reaction in genetic engineering is to

Animal cloning is a difficult process with a low success rate.

Which description best fits the term xenotransplantation?

A transgenic organism is

DNA fingerprinting may be used to establish paternity.Analyze the banding pattern to determine the father of the child.

It is estimated that humans have approximately ______ base pairs in our genome.

What is the first step in producing a transgenic animal?

A View of Life

Basic Chemistry

The Chemistry of Organic Molecules

Cell Structure and Function

Membrane Structure and Function

Metabolism: Energy and Enzymes

Photosynthesis

Cellular Respiration

The Cell Cycle and Cellular Reproduction

Meiosis and Sexual Reproduction

Mendelian Patterns of Inheritance

Molecular Biology of the Gene

Regulation of Gene Expression

Darwin and Evolution

How Populations Evolve

Speciation and Macroevolution

Origin and History of Life

Taxonomy,systematics,and Phylogeny

Viruses,bacteria,and Archaea

Protist Evolution and Diversity

Fungi Evolution and Diversity

Plant Evolution and Diversity

Flowering Plants: Structure and Organization

Flowering Plants: Nutrition and Transport

Flowering Plants: Control of Growth Responses

Flowering Plants: Reproduction

Invertebrate Evolution

Vertebrate Evolution

Human Evolution

Animal Organization and Homeostasis

Circulation and Cardiovascular Systems

The Lymphatic and Immune Systems

Digestive Systems and Nutrition

Respiratory Systems

Body Fluid Regulation and Excretory Systems

Neurons and Nervous Systems

Sense Organs

Locomotion and Support Systems

Hormones and Endocrine Systems

Reproductive Systems

Animal Development

Behavioral Ecology

Population Ecology

Community and Ecosystem Ecology

Major Ecosystems of the Biosphere

Conservation of Biodiversity

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