Exam 8: Rna: Transcription and Processing

Suppose that you have sequenced five genes,including the putative promoter region of each gene,that are involved in the cold acclimation process in Arabidopsis.Each of the five genes is expressed only when the temperature falls below a certain temperature.You know that a specific transcription factor (CBF1)binds somewhere between -200 and -250 of these genes to initiate transcription.How could you use the sequences to determine the DNA sequence motif that is bound by CBF1?

Pulse-chase experiments provided evidence that:

In a chromosome,which of the following is TRUE?

Following splicing,the conserved adenosine nucleotide within the intron displays an unusual array of bonds,including:

Which of the following is/are TRUE about functional RNA?

What would the minimum word (codon)size be if the number of different bases in the mRNA were a)two;b)three;c)five.Give your reasoning.Assume that all other features of the translation of mRNA into protein are the same as what we now know.

Of the three classes of functional RNAs,rRNA and tRNA exist in both eukaryotes and prokaryotes.However,snRNAs exist only in one of these types of organisms.Do snRNAs exist in prokaryotes or eukaryotes? Explain why they are only found in that type of organism.

The conserved adenine nucleotide in introns serves as the:

Which of the following is/are role(s)of the 5′ cap?

The role of most microRNAs within a eukaryotic cell is to:

Template strand DNA and encoded RNA are:

The -10 and -35 boxes found in most bacterial promoter elements were discovered by:

The functional equivalent of the TATA binding protein in prokaryotes is: