Exam 11: Regulation of Gene Expression in Bacteria and Their Viruses

Give the levels of $\beta$ -galactosidase activity ( $\textbf{ high}$ or $\textbf{ low }$ )expected for the following partial diploids for the lac operon. $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ Lactose absent Lactose present a) $I ^ { + } P ^ { + } O ^ { + } Z ^ { - } / I ^ { + } P ^- O ^ { + } Z ^ { + }$ b) $I ^ { + } P ^ { + } O ^ { + } Z ^ { - } / I ^ { - } P ^ { + } O ^ { + } Z ^ { + }$ c) $I ^ { + } P ^ { + } O ^ { \mathrm { C } } Z ^ { - } / I ^ { + } P ^ { + } O ^ { + } Z ^ { + }$ d) $I^- P ^ { + } O ^ { \mathrm { C } } Z ^ { + } / I ^ { + } P ^ { + } O ^ { + } Z ^ { + }$ e) $I ^ { + } P ^ { + } O ^ { \mathrm { C } } Z ^ { + } / I ^ { S } P ^ { + } O ^ { + } Z ^ { + }$

The specific conformation (shape)of DNA binding regulatory proteins is such that these proteins can interact and bind to:

A partial diploid of genotype I^- P⁺ O⁺ Z⁺ / I⁺ P⁺ O⁺ Z^- will show:

In the bacteriophage $\lambda$ ,the cI gene encodes the $\lambda$ repressor,which:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -regulatory molecule must be present at site in DNA (such as promoter)so that transcription occurs

Which is the synthetic inducer Jacob and Monod used to examine the expression of $\beta$ -galactosidase?

A promoter mutation (P^-)results in:

E.coli bacteria are placed into a medium containing both glucose and lactose.Which of the gene products (i.e.,enzymes)listed below do you predict will be "turned on"?

Which of the following is/are NOT part of the lac operon?

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -phenotype of I⁺ O⁺ Z^- / I^- O^C Z^-

In the absence of arabinose,the AraC protein represses the ara operon by binding to:

The attenuator region (AR)of the trp operon is located:

The regulation of operon gene expression by attenuator control is commonly found in:

The lac operon is controlled by:

A null repressor mutation (I^-)results in:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -post-translational control of biosynthetic pathway

In the presence of the repressor molecule and the absence of free tryptophan,the trp operon is:

Any regulatory protein that acts by preventing transcription termination is called a:

In the bacteriophage $\lambda$ ,cro gene encodes the Cro repressor,which:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -regulatory molecule that binds to operator region in DNA