Exam 3: Nucleic Acid Structure and Function

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In a single RNA transcript,polycistronic prokaryotic genes encode __________ protein(s).

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Predict how type I topoisomerases change the supercoil region.

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__________ maintain the length of the chromosome after replication.

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Polycistronic genes that contain a coding sequence for proteins that are only involved in one biochemical process are called

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Predict the cleavage products when DNA is exposed to restriction endonucleases using the figure below. Predict the cleavage products when DNA is exposed to restriction endonucleases using the figure below.

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What mediates the binding of histone proteins to DNA?

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Foreign DNA fragments can be inserted into plasmids using

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In the second temperature phase of PCR,why does the temperature vary from 55 In the second temperature phase of PCR,why does the temperature vary from 55   C to 65   C? C to 65 In the second temperature phase of PCR,why does the temperature vary from 55   C to 65   C? C?

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Compare the three different ways that DNA can be transferred between organisms using plasmids.

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Compare and contrast the steps used to induce a positive supercoil and a negative supercoil.

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Type I topoisomerase activity results in a region of __________ DNA.

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DNA regions rich in A-T are more easily denatured than regions with a higher G-C content.Why is this biologically significant?

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How many base pairs per turn does B-DNA contain?

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This method of analyzing RNA transcripts relies on a predetermined collection of complementary DNA sequences.

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Chargaff's rule is that the amount of

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The figure below shows part of the primary structure of DNA.Identify the nucleoside. The figure below shows part of the primary structure of DNA.Identify the nucleoside.

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DNA strands containing 20 base pairs,40 base pairs,and 60 base pairs were denatured and the results were graphed below.Identify the curve from the 60 base pair DNA strand. DNA strands containing 20 base pairs,40 base pairs,and 60 base pairs were denatured and the results were graphed below.Identify the curve from the 60 base pair DNA strand.

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Which form(s)of DNA exhibit(s)a right-handed helical structure?

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The exons of a single gene often encode for different functional domains of a protein,which can result in

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The DNA of a bacteria was isolated and it was determined that 15% of the DNA is composed of cytosine.What percentage of the DNA is adenine?

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