Exam 3: Nucleic Acid Structure and Function

As DNA unwinds and denatures,absorbance is predicted to

Explain the two methods used to determine that plasmid cloning has been successful.

The most common purpose for cloning a gene sequence using mRNA is to

The mixing and matching of novel genes in eukaryotic cells occurs through

Identify the phase of PCR amplification where DNA is denatured and the strands are separated.

A double helix that crosses itself in a right-handed twist is referred to as a

Which enzyme is used to cleave DNA at specific sequences during the production of recombinant DNA?

In the eukaryotic cell,the coding sequences on a gene are referred to as

Compare the primary and secondary structures of DNA.

When DNA is transcribed into RNA,the __________ strand has the same base sequences as the RNA transcript.

How does the ionic strength of DNA affect the T_m? Explain.

Predict the complementary strand of the following DNA sequence: -ATCTGAATCT-

RNA only contains which of the following bases?

Which of the following binds to DNA using a specific sequence?

Less condensed,gene-rich chromatin is referred to as

Which of the following expresses the relationship among the linking number,twist,and writhe?

In plasmid conjugation,DNA is transferred when

Not all single nucleotide polymorphisms cause a phenotypic change because sometimes the changes occur in the

Increasing the ion concentration increases the T_m because the ions

Several histones can bind to one DNA molecule,forming a repeating unit called a