Question 1

All of the following statements are consistent with CFC except:

Accepted Answer

A)  blood is drawn from one phlebotomy site and returned through another. 
B)  reinfusion to the patient completes one cycle. 
C)  the process of phlebotomy, separation, and reinfusion is uninterrupted. 
D)  separation of components is achieved through centrifugation. 
A)  blood is drawn from one phlebotomy site and returned through another. 
B)  reinfusion to the patient completes one cycle. 
C)  the process of phlebotomy, separation, and reinfusion is uninterrupted. 
D)  separation of components is achieved through centrifugation.

Question 2

_____ testing is performed on platelet apheresis products to make sure that HIV, WNV, and hepatitis C are not present.

Accepted Answer

A)  Serological 
B)  Nucleic acid 
C)  Electrophoretic 
D)  Rapid 
A)  Serological 
B)  Nucleic acid 
C)  Electrophoretic 
D)  Rapid

Question 3

Leukopheresis may be indicated when the white blood cell count exceeds_____  .

Accepted Answer

A)  50,000 per µL 
B)  100,000 per µL 
C)  75,000 per µL 
D)  25,000 per µL 
A)  50,000 per µL 
B)  100,000 per µL 
C)  75,000 per µL 
D)  25,000 per µL

Question 4

What effect do steroids have on leukapheresis?

Accepted Answer

A)  Decrease the vascular pool of granulocytes 
B)  Increase the vascular pool of granulocytes 
C)  Help to separate red blood cells from white blood cells, using specific gravity properties 
D)  Neutralize the effect of toxic granulation in neutrophils 
A)  Decrease the vascular pool of granulocytes 
B)  Increase the vascular pool of granulocytes 
C)  Help to separate red blood cells from white blood cells, using specific gravity properties 
D)  Neutralize the effect of toxic granulation in neutrophils

Question 5

Which of the following conditions would necessitate a plasmapheresis procedure?

Accepted Answer

A)  Sickle-cell anemia 
B)  Barbiturate poisoning 
C)  CML 
D)  Polycythemia vera 
A)  Sickle-cell anemia 
B)  Barbiturate poisoning 
C)  CML 
D)  Polycythemia vera

Question 6

A normal healthy donor undergoes a procedure to obtain platelets that will be transfused to a patient is representative of:

Accepted Answer

A)  therapeutic apheresis collection. 
B)  component apheresis collection. 
C)  whole blood donation. 
D)  None of the above. 
A)  therapeutic apheresis collection. 
B)  component apheresis collection. 
C)  whole blood donation. 
D)  None of the above.

Question 7

Fresh frozen plasma (FFP) is not the optimal product for replacement fluids for a therapeutic plasma exchange procedure. For which patient is FFP the optimal product of choice?

Accepted Answer

A)  Patient with liver disease 
B)  Patient with thrombotic thrombocytopenic purpura (TTP) 
C)  Patient scheduled to undergo an invasive procedure 
D)  All of the above 
A)  Patient with liver disease 
B)  Patient with thrombotic thrombocytopenic purpura (TTP) 
C)  Patient scheduled to undergo an invasive procedure 
D)  All of the above

Question 8

The platelet count of the plateletpheresis donor must be _____ prior to the procedure.

Accepted Answer

A)  >100 F1F1F1S1?F1F1F10 109 per L 
B)  >50 F1F1F1S1?F1F1F10 109 per L 
C)  >150 F1F1F1S1?F1F1F10 109 per L 
D)  >20 F1F1F1S1?F1F1F10 109 per L 
A)  >100 F1F1F1S1?F1F1F10 109 per L 
B)  >50 F1F1F1S1?F1F1F10 109 per L 
C)  >150 F1F1F1S1?F1F1F10 109 per L 
D)  >20 F1F1F1S1?F1F1F10 109 per L

Question 9

In what circumstance is a plasmapheresis donor rejected from donation?

Accepted Answer

A)  Blood pressure = 140/95 
B)  Temperature = 99.5°F 
C)  Serum protein = 5.0 g/dL 
D)  Hematocrit = 40% 
A)  Blood pressure = 140/95 
B)  Temperature = 99.5°F 
C)  Serum protein = 5.0 g/dL 
D)  Hematocrit = 40%

Question 10

The method in which a specific ligand is bound to an insoluble matrix in a column and plasma is perfused over the column with select removal of pathogenic substance and return of patient's plasma is known as:

Accepted Answer

A)  immunoinhibition. 
B)  plasma exchange. 
C)  immunoadsorption. 
D)  elution. 
A)  immunoinhibition. 
B)  plasma exchange. 
C)  immunoadsorption. 
D)  elution.

Question 11

When a sample of whole blood is spun, distinct layers form. What cell line or lines make up the buffy coat?

Accepted Answer

A)  White blood cells only 
B)  White blood cells and platelets 
C)  Red blood cells 
D)  Cellular fragments 
A)  White blood cells only 
B)  White blood cells and platelets 
C)  Red blood cells 
D)  Cellular fragments

Question 12

While undergoing plasmapheresis, the donor experienced numbness around his mouth, which is indicative of citrate toxicity. How can this be treated?

Accepted Answer

A)  Administering exogenous sodium 
B)  Administering exogenous potassium 
C)  Administering exogenous calcium 
D)  Decreasing the extracorporeal volume 
A)  Administering exogenous sodium 
B)  Administering exogenous potassium 
C)  Administering exogenous calcium 
D)  Decreasing the extracorporeal volume

Question 13

What is the physiological cause of citrate toxicity in cytapheresis procedures?

Accepted Answer

A)  The extracorporeal blood volume leaks citrate into the reinfusion site of the donor. 
B)  The anticoagulant in plasma contains citrate, which binds calcium, lowering the body's ionized calcium. 
C)  The anticoagulant in plasma contains citrate, which binds chloride, lowering the anion gap. 
D)  None of the above. 
A)  The extracorporeal blood volume leaks citrate into the reinfusion site of the donor. 
B)  The anticoagulant in plasma contains citrate, which binds calcium, lowering the body's ionized calcium. 
C)  The anticoagulant in plasma contains citrate, which binds chloride, lowering the anion gap. 
D)  None of the above.

Question 14

Which apheresis method carries the additional risk of returning red blood cells to the wrong individual and yielding the smallest volume of selected blood component?

Accepted Answer

A)  IFC 
B)  Manual apheresis 
C)  CFC 
D)  Membrane filtration 
A)  IFC 
B)  Manual apheresis 
C)  CFC 
D)  Membrane filtration

Question 15

All of the following statements are consistent with photopheresis except:

Accepted Answer

A)  the patient is given an oral dose of psoralen, which binds to the DNA of all nucleated cells. 
B)  collected white blood cells are exposed to ultraviolet light, which activates psoralen, preventing replication. 
C)  the patient is given an oral dose of piroxicam, which binds to the RNA of all nucleated cells. 
D)  treated cells are returned to the patient, inducing an immune response against the abnormal lymphocyte clone. 
A)  the patient is given an oral dose of psoralen, which binds to the DNA of all nucleated cells. 
B)  collected white blood cells are exposed to ultraviolet light, which activates psoralen, preventing replication. 
C)  the patient is given an oral dose of piroxicam, which binds to the RNA of all nucleated cells. 
D)  treated cells are returned to the patient, inducing an immune response against the abnormal lymphocyte clone.

Question 16

One unit of apheresed platelets should increase the platelet count_____  .

Accepted Answer

A)  5,000 to 10,000 per µL 
B)  20,000 to 60,000 per µL 
C)  80,000 to 100,000 per µL 
D)  15,000 to 30,000 per µL 
A)  5,000 to 10,000 per µL 
B)  20,000 to 60,000 per µL 
C)  80,000 to 100,000 per µL 
D)  15,000 to 30,000 per µL

Question 17

A donor's estimated total blood volume was determined to be 4,500 mL before a plasmapheresis procedure. The extracorporeal blood volume should not exceed:

Accepted Answer

A)  675 mL. 
B)  335 mL. 
C)  187 mL. 
D)  50 mL. 
A)  675 mL. 
B)  335 mL. 
C)  187 mL. 
D)  50 mL.

Exam 14: Apheresis

All of the following statements are consistent with CFC except:

_____ testing is performed on platelet apheresis products to make sure that HIV, WNV, and hepatitis C are not present.

Leukopheresis may be indicated when the white blood cell count exceeds_____ .

What effect do steroids have on leukapheresis?

Which of the following conditions would necessitate a plasmapheresis procedure?

A normal healthy donor undergoes a procedure to obtain platelets that will be transfused to a patient is representative of:

Fresh frozen plasma (FFP) is not the optimal product for replacement fluids for a therapeutic plasma exchange procedure. For which patient is FFP the optimal product of choice?

The platelet count of the plateletpheresis donor must be _____ prior to the procedure.

In what circumstance is a plasmapheresis donor rejected from donation?

The method in which a specific ligand is bound to an insoluble matrix in a column and plasma is perfused over the column with select removal of pathogenic substance and return of patient's plasma is known as:

When a sample of whole blood is spun, distinct layers form. What cell line or lines make up the buffy coat?

While undergoing plasmapheresis, the donor experienced numbness around his mouth, which is indicative of citrate toxicity. How can this be treated?

What is the physiological cause of citrate toxicity in cytapheresis procedures?

Which apheresis method carries the additional risk of returning red blood cells to the wrong individual and yielding the smallest volume of selected blood component?

All of the following statements are consistent with photopheresis except:

One unit of apheresed platelets should increase the platelet count_____ .

A donor's estimated total blood volume was determined to be 4,500 mL before a plasmapheresis procedure. The extracorporeal blood volume should not exceed:

Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends

Basic Genetics

Fundamentals of Immunology

Concepts in Molecular Biology

The Antiglobulin Test

The Abo Blood Group System

The RH Blood Group System

Blood Group Terminology and the Other Blood Groups

Detection and Identification of Antibodies

Pre-Transfusion Testing

Overview of the Routine Blood Bank Laboratory

Other Technologies and Automation

Donor Screening and Component Preparation

Transfusion Therapy

Cellular Therapy

Transfusion-Transmitted Diseases

Hemolytic Disease of the Fetus and Newborn

Autoimmune Hemolytic Anemias

The Hla System

Relationship Testing

Quality Management

Transfusion Safety and Federal Regulatory Requirements

Utilization Management

Laboratory Information Systems

Medicolegal and Ethical Aspects of Providing Blood Collection and Transfusion Services

Tissue Banking: A New Role for the Transfusion Service

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