Exam 6: Analysis and Characterization of Nucleic Acids and Proteins

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Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

(Multiple Choice)

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Question 21

A northern blot allows you to do which of the following?

(Multiple Choice)

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Question 22

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

(Multiple Choice)

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Question 23

Western blot probes are what type of molecules?

(Multiple Choice)

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Question 24

Which of the following is not a method of transferring nucleic acids from a gel to a membrane substrate?

(Multiple Choice)

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Question 25

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

(Multiple Choice)

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Question 26

How do gene expression arrays differ from comparative genome hybridization (CGH) arrays?

(Multiple Choice)

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Question 27

The entire human genome can be analyzed at one time by which of the following methods?

(Multiple Choice)

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Question 28

A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70^oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?

(Multiple Choice)

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Question 29

A linear molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

(Multiple Choice)

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Question 30

Showing 21 - 30 of 30

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

A northern blot allows you to do which of the following?

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

Western blot probes are what type of molecules?

Which of the following is not a method of transferring nucleic acids from a gel to a membrane substrate?

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

How do gene expression arrays differ from comparative genome hybridization (CGH) arrays?

The entire human genome can be analyzed at one time by which of the following methods?

A linear molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

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