Question 1

HLA is an abbreviation for what term?

Accepted Answer

A)  Histocompatability locus array 
B)  Human leukocyte antibody 
C)  Human leukocyte antigen 
D)  Hepatic lipid antigen 
A)  Histocompatability locus array 
B)  Human leukocyte antibody 
C)  Human leukocyte antigen 
D)  Hepatic lipid antigen

Question 2

The level of detail to which an allele is identified is called which of the following?

Accepted Answer

A)  Resolution 
B)  Ambiguity 
C)  Specificity 
D)  Typing 
A)  Resolution 
B)  Ambiguity 
C)  Specificity 
D)  Typing

Question 3

Which are used in crossmatching for organ transplant?

Accepted Answer

A)  Donor serum and recipient lymphocytes 
B)  Recipient serum and donor lymphocytes 
C)  Donor serum and recipient serum 
D)  Donor lymphocytes and recipient lymphocytes 
A)  Donor serum and recipient lymphocytes 
B)  Recipient serum and donor lymphocytes 
C)  Donor serum and recipient serum 
D)  Donor lymphocytes and recipient lymphocytes

Question 4

Which is a nucleic acid-based test?

Accepted Answer

A)  CDC 
B)  Isoelectric focusing 
C)  Crossmatching 
D)  SSOPH 
A)  CDC 
B)  Isoelectric focusing 
C)  Crossmatching 
D)  SSOPH

Question 5

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue. What is the interpretation of this assay?

Accepted Answer

A)  The lymphocytes express antigens specific for the antibodies in the assay. 
B)  The lymphocytes do not have antigens specific for the antibodies in the assay. 
C)  This is not a valid method for identifying HLA antigens. 
D)  The cells are resistant to complement lysis. 
A)  The lymphocytes express antigens specific for the antibodies in the assay. 
B)  The lymphocytes do not have antigens specific for the antibodies in the assay. 
C)  This is not a valid method for identifying HLA antigens. 
D)  The cells are resistant to complement lysis.

Question 6

HLA specificities were first defined in the laboratory based on which type of methodology?

Accepted Answer

A)  Restriction fragment length polymorphism 
B)  Pulsed field gel electrophoresis 
C)  Amino acid sequencing 
D)  Serology 
A)  Restriction fragment length polymorphism 
B)  Pulsed field gel electrophoresis 
C)  Amino acid sequencing 
D)  Serology

Question 7

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Accepted Answer

A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  Class II antigens 
A)  HLA-A 
B)  Complement C2 
C)  TNF-alpha 
D)  Class II antigens

Question 8

Graft rejection of MHC-identical transplants can be caused by which of the following?

Accepted Answer

A)  The alleles present 
B)  Minor histocompatability antigens 
C)  The haplotype location on chromosome 6 
D)  Gender of the recipient 
A)  The alleles present 
B)  Minor histocompatability antigens 
C)  The haplotype location on chromosome 6 
D)  Gender of the recipient

Question 9

In HLA nomenclature, the letter N indicates what type of allele?

Accepted Answer

A)  An allele with a new antigenic phenotype 
B)  An allele with no reaction to antibody 
C)  A new antigen sequence 
D)  Normal antibody binding 
A)  An allele with a new antigenic phenotype 
B)  An allele with no reaction to antibody 
C)  A new antigen sequence 
D)  Normal antibody binding

Question 10

A woman requires a kidney transplant. Five members of her family would like to be tested to determine if they are eligible donors. Technologists use sequence-specific oligonucleotide probe hybridization methods to perform HLA typing. Which of the following anticoagulants is preferred when collecting blood from the woman and her family members?

Accepted Answer

A)  None 
B)  Heparin 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium polyanethol sulfonate 
A)  None 
B)  Heparin 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium polyanethol sulfonate

Question 11

Which of the following methods that can be used to determine HLA types is only a low-resolution method?

Accepted Answer

A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT 
A)  PCR-SSP 
B)  PCR-SSOP 
C)  CDC 
D)  SBT

HLA is an abbreviation for what term?

The level of detail to which an allele is identified is called which of the following?

Which are used in crossmatching for organ transplant?

Which is a nucleic acid-based test?

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue. What is the interpretation of this assay?

HLA specificities were first defined in the laboratory based on which type of methodology?

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Graft rejection of MHC-identical transplants can be caused by which of the following?

In HLA nomenclature, the letter N indicates what type of allele?

Which of the following methods that can be used to determine HLA types is only a low-resolution method?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

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Exam 15: DNA-Based Tissue Typing

HLA is an abbreviation for what term?

The level of detail to which an allele is identified is called which of the following?

Which are used in crossmatching for organ transplant?

Which is a nucleic acid-based test?

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue. What is the interpretation of this assay?

HLA specificities were first defined in the laboratory based on which type of methodology?

Which are only expressed constitutively on "professional antigen-presenting cells," such as B lymphocytes?

Graft rejection of MHC-identical transplants can be caused by which of the following?

In HLA nomenclature, the letter N indicates what type of allele?

Which of the following methods that can be used to determine HLA types is only a low-resolution method?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Quality Assurance and Quality Control in the Molecular Laboratory

Filters