Question 1

PCR with RFLP analysis and reverse hybridization is the principle of which of the following commercial assays?

Accepted Answer

A)  TRUGENE 
B)  Cleavase fragment length polymorphism 
C)  Amplicor HCV 
D)  Inno-LiPA 
A)  TRUGENE 
B)  Cleavase fragment length polymorphism 
C)  Amplicor HCV 
D)  Inno-LiPA

Question 2

A PCR assay using primers specific for the autolysin gene (lytA) was performed on a sputum specimen from a 10-year-old girl. The specimen was positive, and all controls were acceptable. Which of the following is the best interpretation of these results?

Accepted Answer

A)  Definitive diagnosis of clinically significant Streptococcus pneumoniae pneumonia is made. 
B)  Streptococcus pneumoniae is present, but clinical significance is questionable. 
C)  Mycobacterium tuberculosis is detected, and the girl should be isolated. 
D)  The patient has Chlamydophila pneumoniae pneumonia. 
A)  Definitive diagnosis of clinically significant Streptococcus pneumoniae pneumonia is made. 
B)  Streptococcus pneumoniae is present, but clinical significance is questionable. 
C)  Mycobacterium tuberculosis is detected, and the girl should be isolated. 
D)  The patient has Chlamydophila pneumoniae pneumonia.

Question 3

Which of the following is a genotypic method for typing organisms?

Accepted Answer

A)  Antibiogram patterns 
B)  Pulsed field gel electrophoresis 
C)  Serotyping 
D)  Bacteriophage typing 
A)  Antibiogram patterns 
B)  Pulsed field gel electrophoresis 
C)  Serotyping 
D)  Bacteriophage typing

Question 4

Which of the following is the molecular target for most epidemiological investigations of Mycobacterium tuberculosis?

Accepted Answer

A)  katG 
B)  inhA 
C)  IS6110 
D)  IS481 
A)  katG 
B)  inhA 
C)  IS6110 
D)  IS481

Question 5

Which of the following genes is a target in molecular-based assays and confers resistance to vancomycin in the enterococci?

Accepted Answer

A)  inhA 
B)  mecA 
C)  vanA 
D)  gyrA 
A)  inhA 
B)  mecA 
C)  vanA 
D)  gyrA

PCR with RFLP analysis and reverse hybridization is the principle of which of the following commercial assays?

A PCR assay using primers specific for the autolysin gene (lytA) was performed on a sputum specimen from a 10-year-old girl. The specimen was positive, and all controls were acceptable. Which of the following is the best interpretation of these results?

Which of the following is a genotypic method for typing organisms?

Which of the following is the molecular target for most epidemiological investigations of Mycobacterium tuberculosis?

Which of the following genes is a target in molecular-based assays and confers resistance to vancomycin in the enterococci?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 12: Detection and Identification of Microorganisms

PCR with RFLP analysis and reverse hybridization is the principle of which of the following commercial assays?

A PCR assay using primers specific for the autolysin gene (lytA) was performed on a sputum specimen from a 10-year-old girl. The specimen was positive, and all controls were acceptable. Which of the following is the best interpretation of these results?

Which of the following is a genotypic method for typing organisms?

Which of the following is the molecular target for most epidemiological investigations of Mycobacterium tuberculosis?

Which of the following genes is a target in molecular-based assays and confers resistance to vancomycin in the enterococci?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters