Question 1

Western blot probes are what type of molecules?

Accepted Answer

A)  DNA 
B)  RNA 
C)  protein 
D)  PNA 
A)  DNA 
B)  RNA 
C)  protein 
D)  PNA

Question 2

In order to depurinate DNA after electrophoresis, the gel should be soaked in which of the following?

Accepted Answer

A)  NaOH 
B)  HCl 
C)  Saline sodium citrate 
D)  Formamide 
A)  NaOH 
B)  HCl 
C)  Saline sodium citrate 
D)  Formamide

Question 3

The microarray or chip is an example of which of the following blotting procedures?

Accepted Answer

A)  Southern blot 
B)  Dot blot 
C)  Reverse dot blot 
D)  Western blot 
A)  Southern blot 
B)  Dot blot 
C)  Reverse dot blot 
D)  Western blot

Question 4

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

Accepted Answer

A)  Probe hybridization, restriction enzyme digestion, denaturation, electrophoresis 
B)  Denaturation, electrophoresis, probe hybridization, restriction enzyme digestion 
C)  Restriction enzyme digestion, electrophoresis, denaturation, probe hybridization 
D)  Electrophoresis, restriction enzyme digestion, probe hybridization, denaturation 
A)  Probe hybridization, restriction enzyme digestion, denaturation, electrophoresis 
B)  Denaturation, electrophoresis, probe hybridization, restriction enzyme digestion 
C)  Restriction enzyme digestion, electrophoresis, denaturation, probe hybridization 
D)  Electrophoresis, restriction enzyme digestion, probe hybridization, denaturation

Question 5

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

Accepted Answer

A)  Incomplete electrophoresis of the nucleic acid 
B)  Loss of alkaline phosphatase activity 
C)  Incorrect stringency conditions for probe binding 
D)  Bubbles or incomplete contact between the membrane and the gel 
A)  Incomplete electrophoresis of the nucleic acid 
B)  Loss of alkaline phosphatase activity 
C)  Incorrect stringency conditions for probe binding 
D)  Bubbles or incomplete contact between the membrane and the gel

Question 6

What is star activity?

Accepted Answer

A)  Optimal specific activity of a restriction enzyme 
B)  Nonspecific digestion of DNA by a restriction enzyme 
C)  Degradation of DNA ends 
D)  Re-ligation of cut DNA 
A)  Optimal specific activity of a restriction enzyme 
B)  Nonspecific digestion of DNA by a restriction enzyme 
C)  Degradation of DNA ends 
D)  Re-ligation of cut DNA

Question 7

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

Accepted Answer

A)  Decrease the incubation temperature. 
B)  Decrease the salt concentration. 
C)  Increase the formamide concentration. 
D)  Increase the incubation temperature. 
A)  Decrease the incubation temperature. 
B)  Decrease the salt concentration. 
C)  Increase the formamide concentration. 
D)  Increase the incubation temperature.

Question 8

A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?

Accepted Answer

A)  Decrease formamide 
B)  Increase temperature 
C)  Increase SSC concentration 
D)  Decrease temperature 
A)  Decrease formamide 
B)  Increase temperature 
C)  Increase SSC concentration 
D)  Decrease temperature

Question 9

What is the melting temperature (Tm) of the following hybrid: AGCTATGCCGGCTAGCAC
TCGATACGGCCGATCGTG

Accepted Answer

A)  40oC 
B)  50oC 
C)  58oC 
D)  65oC 
A)  40oC 
B)  50oC 
C)  58oC 
D)  65oC

Question 10

The specificity of western blots depends on what interaction?

Accepted Answer

A)  DNA/RNA complementarity 
B)  RNA/RNA complementarity 
C)  DNA antigens 
D)  Antigen/antibody recognition 
A)  DNA/RNA complementarity 
B)  RNA/RNA complementarity 
C)  DNA antigens 
D)  Antigen/antibody recognition

Western blot probes are what type of molecules?

In order to depurinate DNA after electrophoresis, the gel should be soaked in which of the following?

The microarray or chip is an example of which of the following blotting procedures?

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

What is star activity?

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

What is the melting temperature (T_m) of the following hybrid: AGCTATGCCGGCTAGCAC TCGATACGGCCGATCGTG

The specificity of western blots depends on what interaction?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 6: Analysis and Characterization of Nucleic Acids and Proteins

Western blot probes are what type of molecules?

In order to depurinate DNA after electrophoresis, the gel should be soaked in which of the following?

The microarray or chip is an example of which of the following blotting procedures?

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

What is star activity?

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

What is the melting temperature (Tm) of the following hybrid: AGCTATGCCGGCTAGCAC TCGATACGGCCGATCGTG

The specificity of western blots depends on what interaction?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

What is the melting temperature (T_m) of the following hybrid: AGCTATGCCGGCTAGCAC TCGATACGGCCGATCGTG