Question 1

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

Accepted Answer

A)  20 
B)  30 
C)  40 
D)  50 
A)  20 
B)  30 
C)  40 
D)  50

Question 2

Which of the following will not inhibit RNAses?

Accepted Answer

A)  Guanidine isothiocyanate (GIT
C)  
B)  Tris buffer 
C)  Strong reducing agents 
D)  High-salt buffers 
A)  Guanidine isothiocyanate (GIT
C)  
B)  Tris buffer 
C)  Strong reducing agents 
D)  High-salt buffers

Question 3

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Accepted Answer

A)  Proceed with DNA analysis as necessary. 
B)  Reprecipitate the DNA to remove excess protein. 
C)  Treat the sample with RNAse to remove RNA. 
D)  Dilute the sample to 1:10 and repeat spectrophotometry. 
A)  Proceed with DNA analysis as necessary. 
B)  Reprecipitate the DNA to remove excess protein. 
C)  Treat the sample with RNAse to remove RNA. 
D)  Dilute the sample to 1:10 and repeat spectrophotometry.

Question 4

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

Accepted Answer

A)  Inactivate DNAses 
B)  Measure DNA concentration 
C)  Isolate mitochondria 
D)  Inactivate RNAses 
A)  Inactivate DNAses 
B)  Measure DNA concentration 
C)  Isolate mitochondria 
D)  Inactivate RNAses

Question 5

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

Accepted Answer

A)  Degrade DNA 
B)  Denature all proteins 
C)  Inhibit RNAses 
D)  Separate RNA from DNA 
A)  Degrade DNA 
B)  Denature all proteins 
C)  Inhibit RNAses 
D)  Separate RNA from DNA

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

Which of the following will not inhibit RNAses?

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 4: Nucleic Acid Extraction Methods

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

Which of the following will not inhibit RNAses?

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

DNA-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters