Question 1

Transgenic animals made by pronuclear injection can be limited for studies of the links between genes and disease because

Accepted Answer

A) the transgene allele must be dominant to the normal, endogenous allele and some diseases are caused by recessive mutations. 
B) the transgene allele must be recessive to the normal, endogenous allele and some diseases are caused by dominant mutations. 
C) all diseases are caused by mutations in multiple genes. 
D) diseases are not caused by specific alleles of a gene, but by posttranslational modifications of proteins. 
A) the transgene allele must be dominant to the normal, endogenous allele and some diseases are caused by recessive mutations. 
B) the transgene allele must be recessive to the normal, endogenous allele and some diseases are caused by dominant mutations. 
C) all diseases are caused by mutations in multiple genes. 
D) diseases are not caused by specific alleles of a gene, but by posttranslational modifications of proteins.

Question 2

Huntington disease is caused by expansion of the trinucleotide repeat region of the HD gene that results in the production of a Huntingtin protein with an expanded number of glutamines.An animal model with most features of this syndrome could be created by

Accepted Answer

A) adding a transgene containing a disease-causing mutant allele of the HD gene to a mouse or primate genome. 
B) adding a transgene containing a wild-type copy of the HD gene to a mouse or primate genome. 
C) knocking out both copies of the HD gene from a mouse or primate genome. 
D) knocking out one copy of the HD gene from a mouse or primate genome. 
A) adding a transgene containing a disease-causing mutant allele of the HD gene to a mouse or primate genome. 
B) adding a transgene containing a wild-type copy of the HD gene to a mouse or primate genome. 
C) knocking out both copies of the HD gene from a mouse or primate genome. 
D) knocking out one copy of the HD gene from a mouse or primate genome.

Question 3

The coding sequence of the green fluorescent protein gene (GFP)is placed under the control of an enhancer active in neurons.When this transgene is introduced into flies, GFP will be expressed in

Accepted Answer

A) all neurons or a subset of neurons. 
B) all fly cells that have the transgene. 
C) all cells in the early embryo and then GFP will be silenced. 
D) all cells in the late embryo after neurons form. 
A) all neurons or a subset of neurons. 
B) all fly cells that have the transgene. 
C) all cells in the early embryo and then GFP will be silenced. 
D) all cells in the late embryo after neurons form.

Question 4

Currently the two main vectors for delivery of therapeutic genes for human gene therapy are

Accepted Answer

A) AAV and retroviruses. 
B) AAV and Agrobacterium. 
C) Retroviruses and Agrobacterium. 
D) AAV and P elements. 
A) AAV and retroviruses. 
B) AAV and Agrobacterium. 
C) Retroviruses and Agrobacterium. 
D) AAV and P elements.

Question 5

What is the purpose of the P element in the creation of transgenic Drosophila?

Accepted Answer

A) Efficiently transfer DNA into Drosophila chromosomes. 
B) Transfer DNA into a specific site in the host chromosome. 
C) Transfer DNA only into the nuclei of somatic cells. 
D) Transfer DNA only into primitive eye cells. 
A) Efficiently transfer DNA into Drosophila chromosomes. 
B) Transfer DNA into a specific site in the host chromosome. 
C) Transfer DNA only into the nuclei of somatic cells. 
D) Transfer DNA only into primitive eye cells.

Question 6

Knockin mice can be created using the Cre/loxP system.In this procedure, the loxP sites are used to

Accepted Answer

A) delete the neomycin-resistance marker gene. 
B) recombine the mutation into the genome. 
C) express Cre protein. 
D) flip the orientation of genomic DNA after the mutation is inserted. 
A) delete the neomycin-resistance marker gene. 
B) recombine the mutation into the genome. 
C) express Cre protein. 
D) flip the orientation of genomic DNA after the mutation is inserted.

Question 7

If integration of DNA provided by pronuclear injection occurs after three cell divisions, the resulting mouse will

Accepted Answer

A) not contain the transgene in any cells. 
B) be a mosaic of cells with some cells containing the transgene and others not. 
C) contain the transgene in only germ cells. 
D) contain the transgene in only somatic cells. 
A) not contain the transgene in any cells. 
B) be a mosaic of cells with some cells containing the transgene and others not. 
C) contain the transgene in only germ cells. 
D) contain the transgene in only somatic cells.

Question 8

The FDA-approved GM Atlantic salmon have a growth hormone transgene that is expressed year-round, which is advantageous because

Accepted Answer

A) the salmon is more nutritious. 
B) the salmon achieve their full weight faster. 
C) the salmon produce more offspring. 
D) the salmon are more resistant to infection. 
A) the salmon is more nutritious. 
B) the salmon achieve their full weight faster. 
C) the salmon produce more offspring. 
D) the salmon are more resistant to infection.

Transgenic animals made by pronuclear injection can be limited for studies of the links between genes and disease because

Huntington disease is caused by expansion of the trinucleotide repeat region of the HD gene that results in the production of a Huntingtin protein with an expanded number of glutamines.An animal model with most features of this syndrome could be created by

The coding sequence of the green fluorescent protein gene (GFP)is placed under the control of an enhancer active in neurons.When this transgene is introduced into flies, GFP will be expressed in

Currently the two main vectors for delivery of therapeutic genes for human gene therapy are

What is the purpose of the P element in the creation of transgenic Drosophila?

Knockin mice can be created using the Cre/loxP system.In this procedure, the loxP sites are used to

If integration of DNA provided by pronuclear injection occurs after three cell divisions, the resulting mouse will

The FDA-approved GM Atlantic salmon have a growth hormone transgene that is expressed year-round, which is advantageous because

Genetics: the Study of Biological Information

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

DNA Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Dna

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

The Genetics of Complex Traits

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Exam 18: Manipulating the Genomes of Eukaryotes

Transgenic animals made by pronuclear injection can be limited for studies of the links between genes and disease because

Huntington disease is caused by expansion of the trinucleotide repeat region of the HD gene that results in the production of a Huntingtin protein with an expanded number of glutamines.An animal model with most features of this syndrome could be created by

The coding sequence of the green fluorescent protein gene (GFP)is placed under the control of an enhancer active in neurons.When this transgene is introduced into flies, GFP will be expressed in

Currently the two main vectors for delivery of therapeutic genes for human gene therapy are

What is the purpose of the P element in the creation of transgenic Drosophila?

Knockin mice can be created using the Cre/loxP system.In this procedure, the loxP sites are used to

If integration of DNA provided by pronuclear injection occurs after three cell divisions, the resulting mouse will

The FDA-approved GM Atlantic salmon have a growth hormone transgene that is expressed year-round, which is advantageous because

Genetics: the Study of Biological Information

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

DNA Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Dna

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

The Genetics of Complex Traits

Filters