Question 1

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

Accepted Answer

A) Branched chain PCR 
B) Ligase chain reaction 
C) Nucleic acid sequence based amplification 
D) In situ amplification 
A) Branched chain PCR 
B) Ligase chain reaction 
C) Nucleic acid sequence based amplification 
D) In situ amplification

Question 2

What is an advantage of nucleic acid sequence based amplification (NASBA)?

Accepted Answer

A) It is faster than PCR. 
B) Primers are not necessary. 
C) The reaction is isothermal. 
D) Product can be detected in real time. 
A) It is faster than PCR. 
B) Primers are not necessary. 
C) The reaction is isothermal. 
D) Product can be detected in real time.

Question 3

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

Accepted Answer

A) Nucleic acid probe techniques. 
B) Nucleic acid amplification techniques. 
C) The sensitivities of the two techniques are approximately equal. 
D) It depends on the particular technique as some amplification techniques are more sensitive than others. 
A) Nucleic acid probe techniques. 
B) Nucleic acid amplification techniques. 
C) The sensitivities of the two techniques are approximately equal. 
D) It depends on the particular technique as some amplification techniques are more sensitive than others.

Question 4

The three basic steps of polymerase chain reaction (PCR)are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Accepted Answer

A) Primer design-oligonucleotide of 15- 30 base pairs 
B) Primer extension-10 minutes during each cycle 
C) Primer annealing-95° C for 1 minute 
D) Denaturation-50° C for 5 minutes 
E) Primer extension-37° C when Taq polymerase is used 
A) Primer design-oligonucleotide of 15- 30 base pairs 
B) Primer extension-10 minutes during each cycle 
C) Primer annealing-95° C for 1 minute 
D) Denaturation-50° C for 5 minutes 
E) Primer extension-37° C when Taq polymerase is used

Question 5

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

Accepted Answer

A) True 
 B)False

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

What is an advantage of nucleic acid sequence based amplification (NASBA)?

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

The three basic steps of polymerase chain reaction (PCR)are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

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Exam 13: Amplification Techniques

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

What is an advantage of nucleic acid sequence based amplification (NASBA)?

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

The three basic steps of polymerase chain reaction (PCR)are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

Filters