Question 1

Approximately how many digenic mutant combinations result in a lethal phenotype in budding yeast?

Accepted Answer

A)  1,000 
B)  2,500 
C)  25,000 
D)  100,000 
E)  250,000 
A)  1,000 
B)  2,500 
C)  25,000 
D)  100,000 
E)  250,000

Question 2

siRNA contains a stem loop.

Accepted Answer

A) True 
 B)False

Question 3

Why is Saccharomyces cerevisiae a useful model organism for functional genetics?

Accepted Answer

A)  It has approximately the same number of protein coding genes as humans do. 
B)  It has the smallest genome of any organism. 
C)  The function of every one of its genes is known. 
D)  It has the smallest genome of any eukaryote. 
E)  It can exist as a haploid or diploid. 
A)  It has approximately the same number of protein coding genes as humans do. 
B)  It has the smallest genome of any organism. 
C)  The function of every one of its genes is known. 
D)  It has the smallest genome of any eukaryote. 
E)  It can exist as a haploid or diploid.

Question 4

Budding yeast with the genotype A+B+ is haploid.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following is an important control component when using an RNAi library to screening breast cancer cell lines for genes required for cell profileration?

Accepted Answer

A)  genomic DNA 
B)  Cy3 dyes 
C)  Cy5 dyes 
D)  a normal mammary cell line 
E)  barcodes 
A)  genomic DNA 
B)  Cy3 dyes 
C)  Cy5 dyes 
D)  a normal mammary cell line 
E)  barcodes

Question 6

Up to 85% of introduced deletion casettes will undergo homologous recombination in budding yeast.

Accepted Answer

A) True 
 B)False

Question 7

One pair of PCR primers can amplify any of the barcodes in the genome-wide yeast deletion set.

Accepted Answer

A) True 
 B)False

Question 8

RNAi techniques completely knock-out the transcription of their target gene.

Accepted Answer

A) True 
 B)False

Question 9

Where should barcodes be placed in a gene deletion cassette?

Accepted Answer

A)  flanking the selectable marker 
B)  flanking one of the homologous fragments 
C)  flanking both homologous fragments 
D)  in the middle of the selectable marker 
E)  in the middle of the homologous fragment 
A)  flanking the selectable marker 
B)  flanking one of the homologous fragments 
C)  flanking both homologous fragments 
D)  in the middle of the selectable marker 
E)  in the middle of the homologous fragment

Question 10

If A- is the genotype of a query strain,the genotype that is tested for synthetic lethality in a synthetic genetic array is:

Accepted Answer

A)  A-A- 
B)  A-B+ 
C)  A-B- 
D)  A+A- 
E)  A-A-B- B- 
A)  A-A- 
B)  A-B+ 
C)  A-B- 
D)  A+A- 
E)  A-A-B- B-

Question 11

Barcodes are used in knock-out and knock-down screen in both yeast cells and human cells.

Accepted Answer

A) True 
 B)False

Question 12

In a synthetic lethal,which of the following phenotypes is most likely to be non-viable?

Accepted Answer

A)  A+B+ 
B)  A-B+ 
C)  A+B- 
D)  A-B- 
E)  A+A-B+ B+ 
A)  A+B+ 
B)  A-B+ 
C)  A+B- 
D)  A-B- 
E)  A+A-B+ B+

Question 13

Because it cannot be automated,image analysis is the major limitation in phenotyping genome-wide yeast deletion analysis.

Accepted Answer

A) True 
 B)False

Question 14

A diagram showing all of the functional overlap between genes of a given species can be used to describe its

Accepted Answer

A)  synthetic array. 
B)  genome architecture. 
C)  genotype-phenotype relationships. 
D)  phenological array. 
E)  phylogenetic history. 
A)  synthetic array. 
B)  genome architecture. 
C)  genotype-phenotype relationships. 
D)  phenological array. 
E)  phylogenetic history.

Question 15

You grow a barcoded yeast deletion set at 4°C and then a copy of the same set at 25°C.Barcodes from the 4°C sample and 25°C are labeled with a green fluorochrome and a red fluorochrome,respectively.How will you identify genes required for growth specifically at cold temperatures?

Accepted Answer

A)  separate the PCR products on an agarose gel and look for red fluorescent bands 
B)  separate the PCR products on an agarose gel and look for yellow fluorescent bands 
C)  hybridize the products to a microarray and look for red spots 
D)  hybridize the products to a microarray and look for green spots 
E)  separate the PCR products on an agarose gel and look for green fluorescent bands 
A)  separate the PCR products on an agarose gel and look for red fluorescent bands 
B)  separate the PCR products on an agarose gel and look for yellow fluorescent bands 
C)  hybridize the products to a microarray and look for red spots 
D)  hybridize the products to a microarray and look for green spots 
E)  separate the PCR products on an agarose gel and look for green fluorescent bands

Question 16

Two crossovers are usually involved when using a yeast deletion cassette to knock-out a gene.

Accepted Answer

A) True 
 B)False

Question 17

Genetic buffering describes

Accepted Answer

A)  the ability of cells to accumulate mutations. 
B)  loss of mutations through repair and recombination. 
C)  the accumulation of extra,non-coding DNA in eukaryotic genomes. 
D)  the ability of one gene to mask variability in another gene. 
E)  the expansion of introns throughout evolution. 
A)  the ability of cells to accumulate mutations. 
B)  loss of mutations through repair and recombination. 
C)  the accumulation of extra,non-coding DNA in eukaryotic genomes. 
D)  the ability of one gene to mask variability in another gene. 
E)  the expansion of introns throughout evolution.

Question 18

What is the benefit of using barcodes in genome-wide deletion screens?

Accepted Answer

Barcodes allow individual DNA molecules

Question 19

Which of the following statements about shRNA is not true?

Accepted Answer

A)  shRNAs are synthetic 19-22bp dsRNAs. 
B)  shRNA encodes a substrate of RISC. 
C)  shRNAs encodes ssRNA. 
D)  shRNA is used to produce siRNA. 
E)  shRNA can be packaged into a virus. 
A)  shRNAs are synthetic 19-22bp dsRNAs. 
B)  shRNA encodes a substrate of RISC. 
C)  shRNAs encodes ssRNA. 
D)  shRNA is used to produce siRNA. 
E)  shRNA can be packaged into a virus.

Question 20

Retroviruses can be used for targeted gene silencing in mammal cells.

Accepted Answer

A) True 
 B)False

Exam 21: Genetics of Complex Traits

Approximately how many digenic mutant combinations result in a lethal phenotype in budding yeast?

siRNA contains a stem loop.

Why is Saccharomyces cerevisiae a useful model organism for functional genetics?

Budding yeast with the genotype A+B+ is haploid.

Which of the following is an important control component when using an RNAi library to screening breast cancer cell lines for genes required for cell profileration?

Up to 85% of introduced deletion casettes will undergo homologous recombination in budding yeast.

One pair of PCR primers can amplify any of the barcodes in the genome-wide yeast deletion set.

RNAi techniques completely knock-out the transcription of their target gene.

Where should barcodes be placed in a gene deletion cassette?

If A- is the genotype of a query strain,the genotype that is tested for synthetic lethality in a synthetic genetic array is:

Barcodes are used in knock-out and knock-down screen in both yeast cells and human cells.

In a synthetic lethal,which of the following phenotypes is most likely to be non-viable?

Because it cannot be automated,image analysis is the major limitation in phenotyping genome-wide yeast deletion analysis.

A diagram showing all of the functional overlap between genes of a given species can be used to describe its

You grow a barcoded yeast deletion set at 4°C and then a copy of the same set at 25°C.Barcodes from the 4°C sample and 25°C are labeled with a green fluorochrome and a red fluorochrome,respectively.How will you identify genes required for growth specifically at cold temperatures?

Two crossovers are usually involved when using a yeast deletion cassette to knock-out a gene.

Genetic buffering describes

What is the benefit of using barcodes in genome-wide deletion screens?

Which of the following statements about shRNA is not true?

Retroviruses can be used for targeted gene silencing in mammal cells.

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters

Budding yeast with the genotype A⁺B⁺ is haploid.