Question 1

In the yeast two-hybrid system,the Gal4 DNA binding domain is unable to activate transcription when it is bound to a promoter.

Accepted Answer

A) True 
 B)False

Question 2

Given a set of genes that you know to be more abundant in tumour than in normal tissues,design an experiment to identify networks of protein-protein interactions that exist in malignant tumours,but not in normal tissues.How would the network analysis affect your choice of proteins to target with new chemotherapy drugs?

Accepted Answer

Affinity capture,followed by mass spectr

Question 3

The intracellular environment of yeast is sufficiently similar to animal cells that any protein interaction that occurs in a yeast cell will also occur in an animal cell.

Accepted Answer

A) True 
 B)False

Question 4

An application of protein arrays is

Accepted Answer

A)  screening thousands of proteins to identify substrates of a particular kinase 
B)  identifying interacting proteins by parallel sequencing 
C)  detecting protein interactions through two-hybrid reporter gene activation 
D)  detecting differential protein expression 
E)  structural determination through x-ray analysis 
A)  screening thousands of proteins to identify substrates of a particular kinase 
B)  identifying interacting proteins by parallel sequencing 
C)  detecting protein interactions through two-hybrid reporter gene activation 
D)  detecting differential protein expression 
E)  structural determination through x-ray analysis

Question 5

The structural diversity of proteins in a cell is greatly increased by alternative splicing and posttranslational modifications.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following can be used in mass spectrometry for quantifying differences in protein abundance in a comparison of two samples?

Accepted Answer

A)  relative fluorescent intensity 
B)  relative gamma emissions 
C)  relative beta emissions 
D)  relative height of peaks in the mass spectrum 
E)  relative increases in molecular mass 
A)  relative fluorescent intensity 
B)  relative gamma emissions 
C)  relative beta emissions 
D)  relative height of peaks in the mass spectrum 
E)  relative increases in molecular mass

Question 7

A mass analyzer separates ions based on their mass and charge.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following properties of a gene or proteins is not affected by alternative splicing?

Accepted Answer

A)  CpG methylation 
B)  protein structure 
C)  subcellular localization 
D)  enzymatic activity 
E)  protein stability 
A)  CpG methylation 
B)  protein structure 
C)  subcellular localization 
D)  enzymatic activity 
E)  protein stability

Question 9

Yeast two-hybrid experiments can only be used to find proteins that interact with transcription factors.

Accepted Answer

A) True 
 B)False

Question 10

In a typical yeast two-hybrid experiment,which of the following is true of a cell that contains an interaction between bait and prey?

Accepted Answer

A)  No growth on media that contains His,and no growth on media that contains Gal 
B)  No growth on media that lacks His,and no growth on media that lacks Gal 
C)  Growth on media that lacks His,and no growth on media that contains His 
D)  Growth on media that contains His,and no growth on media that lacks His 
E)  Growth on media that lacks His,and growth on media that contains His 
A)  No growth on media that contains His,and no growth on media that contains Gal 
B)  No growth on media that lacks His,and no growth on media that lacks Gal 
C)  Growth on media that lacks His,and no growth on media that contains His 
D)  Growth on media that contains His,and no growth on media that lacks His 
E)  Growth on media that lacks His,and growth on media that contains His

Question 11

Hub proteins are proteins that interact with a large number of other proteins.

Accepted Answer

A) True 
 B)False

Question 12

Protein arrays are often made using

Accepted Answer

A)  96 well plates 
B)  agar medium 
C)  microscope slides 
D)  petri dishes 
E)  1536 well plates 
A)  96 well plates 
B)  agar medium 
C)  microscope slides 
D)  petri dishes 
E)  1536 well plates

Question 13

Design an experiment to identify proteins that are more abundant in malignant tumours,but not in normal tissues.

Accepted Answer

Extract proteins from both the normal sa

Question 14

Ionization is required in mass spectrometry to allow formation of stable ionic bonds between peptides.

Accepted Answer

A) True 
 B)False

Question 15

The nuclear pore complex transports mRNA but not proteins.

Accepted Answer

A) True 
 B)False

Question 16

Affinity purification is often involved in the production of protein microarrays.

Accepted Answer

A) True 
 B)False

Question 17

Yeast two-hybrid screens may produce false-positives.

Accepted Answer

A) True 
 B)False

Question 18

Mass spectrometers operate under high vacuum.

Accepted Answer

A) True 
 B)False

Question 19

The centrality-lethality rule states that

Accepted Answer

A)  lethality of cancer cells is central to their evolutionary persistence. 
B)  yeast colonies nearest to the center of a petri dish have the lowest viability. 
C)  the amino acids at the center of a folded protein can cause lethality if unfolding occurs. 
D)  no two proteins at the center of a protein interaction network can exist in the same cell. 
E)  hub proteins are likely to be essential for viability. 
A)  lethality of cancer cells is central to their evolutionary persistence. 
B)  yeast colonies nearest to the center of a petri dish have the lowest viability. 
C)  the amino acids at the center of a folded protein can cause lethality if unfolding occurs. 
D)  no two proteins at the center of a protein interaction network can exist in the same cell. 
E)  hub proteins are likely to be essential for viability.

Question 20

What is the role of HIS3 in yeast two-hybrid experiments?

Accepted Answer

A)  It is a reporter gene. 
B)  It is required for growth in media that contain histidine. 
C)  It activates transcription. 
D)  It binds DNA. 
E)  It acts as a bait for other proteins. 
A)  It is a reporter gene. 
B)  It is required for growth in media that contain histidine. 
C)  It activates transcription. 
D)  It binds DNA. 
E)  It acts as a bait for other proteins.

Exam 20: Variation and Selection in Populations

In the yeast two-hybrid system,the Gal4 DNA binding domain is unable to activate transcription when it is bound to a promoter.

The intracellular environment of yeast is sufficiently similar to animal cells that any protein interaction that occurs in a yeast cell will also occur in an animal cell.

An application of protein arrays is

The structural diversity of proteins in a cell is greatly increased by alternative splicing and posttranslational modifications.

Which of the following can be used in mass spectrometry for quantifying differences in protein abundance in a comparison of two samples?

A mass analyzer separates ions based on their mass and charge.

Which of the following properties of a gene or proteins is not affected by alternative splicing?

Yeast two-hybrid experiments can only be used to find proteins that interact with transcription factors.

In a typical yeast two-hybrid experiment,which of the following is true of a cell that contains an interaction between bait and prey?

Hub proteins are proteins that interact with a large number of other proteins.

Protein arrays are often made using

Design an experiment to identify proteins that are more abundant in malignant tumours,but not in normal tissues.

Ionization is required in mass spectrometry to allow formation of stable ionic bonds between peptides.

The nuclear pore complex transports mRNA but not proteins.

Affinity purification is often involved in the production of protein microarrays.

Yeast two-hybrid screens may produce false-positives.

Mass spectrometers operate under high vacuum.

The centrality-lethality rule states that

What is the role of HIS3 in yeast two-hybrid experiments?

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

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