Question 1

A mass spectrometer can be used to identify a protein only if peptides or proteins with the same sequence are already in a database.

Accepted Answer

A) True 
 B)False

Question 2

The nuclear pore complex is formed by a single large protein that undergoes extensive alternative splicing prior to translation.

Accepted Answer

A) True 
 B)False

Question 3

In high throughput yeast two-hybrid assays,bait and prey strains are of the same mating type.

Accepted Answer

A) True 
 B)False

Question 4

In mass spectrometry,m/z represents

Accepted Answer

A)  mass/charge. 
B)  mass/peak height. 
C)  mass/abundance. 
D)  mass/size. 
E)  mass/interactions. 
A)  mass/charge. 
B)  mass/peak height. 
C)  mass/abundance. 
D)  mass/size. 
E)  mass/interactions.

Question 5

What is an advantage of affinity purification-based methods over yeast two-hybrid (Y2H)methods,when screening for interacting proteins?

Accepted Answer

A)  Affinity methods isolate molecules from their native context and do not require recombinant protein production. 
B)  Affinity methods use fusion proteins rather than antibodies,which are more expensive to produce. 
C)  Affinity methods,but not Y2H,can be used to find proteins that interact with transcription factors. 
D)  Proteins used in affinity methods do not need to be properly folded. 
E)  Affinity methods can be applied to proteins from a wider range of eukaryotes than can be used in Y2H. 
A)  Affinity methods isolate molecules from their native context and do not require recombinant protein production. 
B)  Affinity methods use fusion proteins rather than antibodies,which are more expensive to produce. 
C)  Affinity methods,but not Y2H,can be used to find proteins that interact with transcription factors. 
D)  Proteins used in affinity methods do not need to be properly folded. 
E)  Affinity methods can be applied to proteins from a wider range of eukaryotes than can be used in Y2H.

Question 6

What is the relevance of a collision cell in mass spectrometry?

Accepted Answer

A)  It increases the precision of the analysis. 
B)  It is used to break apart large protein complexes prior to mass spectrometry. 
C)  It is the site of undesirable reactions that can reduce accuracy. 
D)  It is the primary site of ionization. 
E)  It rejoins peptide fragments into their original configuration. 
A)  It increases the precision of the analysis. 
B)  It is used to break apart large protein complexes prior to mass spectrometry. 
C)  It is the site of undesirable reactions that can reduce accuracy. 
D)  It is the primary site of ionization. 
E)  It rejoins peptide fragments into their original configuration.

Question 7

Why is trypsin normally used in protein mass spectrometry?

Accepted Answer

A)  It is a positive control found in almost all animal cells. 
B)  It is used as a standard to define the upper and lower boundaries of mass sensitivity. 
C)  It cuts the protein into smaller peptides,which can be more accurately analyzed than longer fragments. 
D)  It is a useful general capture molecule for affinity purification. 
E)  It is an effective antibiotic to suppress contamination from microbes. 
A)  It is a positive control found in almost all animal cells. 
B)  It is used as a standard to define the upper and lower boundaries of mass sensitivity. 
C)  It cuts the protein into smaller peptides,which can be more accurately analyzed than longer fragments. 
D)  It is a useful general capture molecule for affinity purification. 
E)  It is an effective antibiotic to suppress contamination from microbes.

Question 8

Which of the following is not an example of a molecular machine that is comprised of many proteins?

Accepted Answer

A)  DNA repair complex 
B)  proteasome 
C)  nuclear pore complex 
D)  proteome 
E)  spliceosome 
A)  DNA repair complex 
B)  proteasome 
C)  nuclear pore complex 
D)  proteome 
E)  spliceosome

A mass spectrometer can be used to identify a protein only if peptides or proteins with the same sequence are already in a database.

The nuclear pore complex is formed by a single large protein that undergoes extensive alternative splicing prior to translation.

In high throughput yeast two-hybrid assays,bait and prey strains are of the same mating type.

In mass spectrometry,m/z represents

What is an advantage of affinity purification-based methods over yeast two-hybrid (Y2H)methods,when screening for interacting proteins?

What is the relevance of a collision cell in mass spectrometry?

Why is trypsin normally used in protein mass spectrometry?

Which of the following is not an example of a molecular machine that is comprised of many proteins?

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters

Exam 20: Variation and Selection in Populations

A mass spectrometer can be used to identify a protein only if peptides or proteins with the same sequence are already in a database.

The nuclear pore complex is formed by a single large protein that undergoes extensive alternative splicing prior to translation.

In high throughput yeast two-hybrid assays,bait and prey strains are of the same mating type.

In mass spectrometry,m/z represents

What is an advantage of affinity purification-based methods over yeast two-hybrid (Y2H)methods,when screening for interacting proteins?

What is the relevance of a collision cell in mass spectrometry?

Why is trypsin normally used in protein mass spectrometry?

Which of the following is not an example of a molecular machine that is comprised of many proteins?

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters