Exam 13: Regulation of Gene Expression in Bacteria and Bacteriophage

Which enzyme cuts dsRNA into 21- 25 bp fragments during RNAi?

_____binds to siRNAs and then either blocks translation or destroys mRNA that is complementary to the siRNA.

The enhancers that are present near a gene are present in every cell in an organism, yet these enhancers can have tissue- specific effects on gene expression. Explain how enhancers could have tissue- specific effects on gene expression.

Enzymes that create epigenetic marks are sometimes referred to as writers, whereas enzymes that eliminate epigenetic marks are called_________ .

What effect does methylation of CpG islands have on human promoters?

What are three proteins or groups of proteins you would expect to find bound to the core promoter region in eukaryotes?

Which protein binds to the silencer sequence and promotes transcriptional silencing in the presence of glucose?

Which of the following is not involved in control of gene expression in eukaryotes?

dsRNA is detected and cleaved by?

What gene is critical for establishment of X- inactivation in mammals?

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80. What effect would you expect to see in the absence of galactose?

You have identified a mutation in a gene that seems to decrease transcription of another gene 2000 bp away from the mutation site. What regulatory sequence, which may be found within another gene, has likely been mutated in this instance?

Histone deacetylase (HDAC) inhibitors are commonly used as mood stabilizers or in the treatment of neurodegenerative diseases. What effect would a HDAC inhibitor have on the target cells?

Typically, methylation of nucleosome N- terminal tails leads to_________ .

Explain how genomic imprinting can effect gene expression in mammalian embryos?