Exam 21: Gene Expression: I the Genetic Code and Transcription

With respect to RNA processing, which of the following is false?

Which of the following enzymes is associated with "transcription factories" in the nuclease?

An agent that would work well as an antibiotic against prokaryotic infection would target the

A more contemporary technique for identification of promoter sequences is the assay.

An individual has been infected with a particular retrovirus. In order for the latent virus DNA to be inherited by subsequent generations stemming from this individual, it must be found

Transcription of ribosomal RNA is primarily the function of

The DNA technique used to initially identity promoter sequences was

You are wishing to design a drug that acts against a recently identified retrovirus. Based upon what you know about retroviruses, which of the following would be the best target for specifically interrupting the viral life cycle in eukaryotic cells?

With regard to RNA polymerase proofreading ability, which of the following is CORRECT?

You expect that a mutation in E. coli that was recently isolated results in loss of enzyme R activity. In looking for the gene you find comparable sequences. Using an in vitro transcription translation system, you use the gene obtained from the mutant as well as a wild type E. coli as a control. Following protein isolation and gel electrophoresis, you observe that the wild type E. coli produces a protein that is 35K in size, corresponding to enzyme R. However, the mutant strain produces a protein that is 25K in size. What does this information suggest?

- driven promoters do not contain TATA boxes or BREs.

Which of the following is NOT a reason for the importance of mRNA processing in eukaryotes?

A change in a nucleotide sequence that results in addition or deletion of a single nucleotide and largely changes the amino acid sequence of the resulting peptide is known as a

If you were designing a method to specifically inhibit prokaryotic transcription, but not eukaryotic transcription, interfering with which of the following would work best?

The technique initially used in the identification of promoter sequences was .

Examine the following double- stranded DNA sequence: A- T- G- T- G- G- C- C- A- C- T- A- T- A- A- T- G- A T- A- C- A- C- C- G- G- T- G- A- T- A- T- T- A- C- T Which strand is the coding strand, and where is the N- terminal end of the resulting peptide located?

Answer the questions below based on this diagram: 5'- AUGAUGAUGAUG- 3' This RNA transcript was generated using a synthetic DNA template. The letters B, D, E, F, G, and H have been selected to represent different amino acids. Assume that each different group of codons is a different amino acid. -If the genetic code were overlapping by one nucleotide, using three nucleotides to encode an amino acid, then the resulting amino acid sequence using this sequence would be