Exam 11: Genetics of Bacteria and Archaea

F⁻strains of Escherichia coli

When UV radiation damage occurs, DNA repair occurs only in the absence of template instruction.

Many Bacteria isolated from nature are natural lysogens.

You work for a biotechnology company that uses Streptomyces strains to produce pharmaceutical products. A phage has infected and killed some of your Streptomyces strains during production, resulting in dramatically decreased yields. To protect the strains from infection you propose to

The uptake of free DNA from the environment ________, while transfer of DNA with cell-to-cell contact would most likely result in ________.

Which of the following features are common to transformation, transduction, and conjugation? (1) unidirectional transfer of genes (2) incomplete gene transfer (3) homologous recombination (4) meiosis occurring in the recipient

Which of the following is NOT required for homologous recombination?

Bacterial mating (or conjugation) is a bidirectional process where nucleic acids (DNA or RNA) are transferred between two cells.

Explain how electroporation works and the significance of the procedure for genetic experiments.

Almost all plasmids are double-stranded DNA.

Which of the following is TRUE of competence?

Why is the outcome of a base-pair substitution mutation variable? List and describe the possible outcomes of a base-pair substitution mutation in your answer.

In specialized transduction, as exemplified by lambda phage in E. coli, transduction occurs at high efficiency for only a restricted group of genes near the insertion site of lambda.

The production of a functional gene product by transforming bacteria that lack a lacZ gene with a plasmid containing a lacZ gene is known as

What is one of the changes that occur when a cell contains an F plasmid that is NOT integrated into the chromosome?

The enzyme transposase may be coded for by insertion sequences on a

High-efficiency, natural transformation

You have performed the following mating experiment using Hfr and F-strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ) × F⁻ (thr⁻ leu⁻ gal⁻ strʳ). Which of the following selective media would you use to score recombinant colonies?

Intercalating agents, like acridine orange and ethidium bromide, lead to mutagenesis by pushing DNA base pairs apart, which can lead to insertions or deletions.

Plasmids that govern their own transfer are known as