Question 1

Match the chemiluminescent format with the appropriate description.
-Competitive chemiluminescence

Accepted Answer

A) A fixed amount of labeled antigen competes with unlabeled antigen from a patient specimen for a limited number of antibody-binding sites. 
B) The sample antigen binds to an antibody fixed onto a solid phase; a second antibody, labeled with a chemiluminescent label, binds to the antigen-antibody complex on the solid phase. 
C) A fixed amount of labeled antibody competes with unlabeled antibody from a patient specimen for a limited number of antigen-binding sites. 
D) The sample antibody binds to an antigen fixed onto a solid phase; a second antigen, labeled with a chemiluminescent label, binds to the antigen-antibody complex on the solid phase. 
A) A fixed amount of labeled antigen competes with unlabeled antigen from a patient specimen for a limited number of antibody-binding sites. 
B) The sample antigen binds to an antibody fixed onto a solid phase; a second antibody, labeled with a chemiluminescent label, binds to the antigen-antibody complex on the solid phase. 
C) A fixed amount of labeled antibody competes with unlabeled antibody from a patient specimen for a limited number of antigen-binding sites. 
D) The sample antibody binds to an antigen fixed onto a solid phase; a second antigen, labeled with a chemiluminescent label, binds to the antigen-antibody complex on the solid phase.

Question 2

Chemiluminescent enzyme labels often used in indirect procedures are

Accepted Answer

A) alkaline phosphatase. 
B) horseradish peroxidase. 
C) beta-galactosidase. 
D) All of the above. 
A) alkaline phosphatase. 
B) horseradish peroxidase. 
C) beta-galactosidase. 
D) All of the above.

Question 3

When using a fluorescent substance or dye, the color of the emitted light depends on the nature of the substance.

Accepted Answer

A) True 
 B)False

Question 4

Chemiluminescence

Accepted Answer

A) has excellent sensitivity and dynamic range. 
B) does not require sample radiation. 
C) uses unstable chemiluminescent reagents and conjugates. 
D) Both A and B. 
A) has excellent sensitivity and dynamic range. 
B) does not require sample radiation. 
C) uses unstable chemiluminescent reagents and conjugates. 
D) Both A and B.

Match the chemiluminescent format with the appropriate description. -Competitive chemiluminescence

Chemiluminescent enzyme labels often used in indirect procedures are

When using a fluorescent substance or dye, the color of the emitted light depends on the nature of the substance.

Chemiluminescence

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Rapid Testing

Precipitation and Particle Agglutination Methods

Electrophoresis Techniques

Flow Cytometry

Molecular Laboratory Techniques

Infectious Diseases: Traditional and Alternate Diagnostic Techniques

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Primary and Acquired Immune Deficiency Syndromes

Hypersensitivity Reactions

Immunoproliferative Disorders

Tolerance, Autoimmunity, and Autoimmune Diseases

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Transplantation: Hla, Solid Organ, and Hematopoietic Stem Cells

Tumor Immunology and Up-To-Date Applications of Next-Generation Sequencing

Primer on Vaccines

Filters

Exam 12: Labeling Techniques in Immunoassay

Match the chemiluminescent format with the appropriate description. -Competitive chemiluminescence

Chemiluminescent enzyme labels often used in indirect procedures are

When using a fluorescent substance or dye, the color of the emitted light depends on the nature of the substance.

Chemiluminescence

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Rapid Testing

Precipitation and Particle Agglutination Methods

Electrophoresis Techniques

Flow Cytometry

Molecular Laboratory Techniques

Infectious Diseases: Traditional and Alternate Diagnostic Techniques

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Primary and Acquired Immune Deficiency Syndromes

Hypersensitivity Reactions

Immunoproliferative Disorders

Tolerance, Autoimmunity, and Autoimmune Diseases

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Transplantation: Hla, Solid Organ, and Hematopoietic Stem Cells

Tumor Immunology and Up-To-Date Applications of Next-Generation Sequencing

Primer on Vaccines

Filters