Question 1

The eukaryotic TATA-binding protein (TBP) functions in a manner similar to

Accepted Answer

A)  sigma ( σ ) factor in E. coli. 
B)  rho ( ρ ) factor in E. coli. 
C)  lac I in E. coli. 
D)  CAP in E. coli. 
A)  sigma ( σ ) factor in E. coli. 
B)  rho ( ρ ) factor in E. coli. 
C)  lac I in E. coli. 
D)  CAP in E. coli.

Question 2

Which of the following is true about riboswitches:

Accepted Answer

A)  They have a molecule sensing domain called an aptamer 
B)  There are known pathogens that use riboswitches as part of their mechanism 
C)  They respond to specific molecules and control translation in a way that is often similar to transcription attenuation 
D)  They contain a sensing domain and a decision making domain 
E)  All of these 
A)  They have a molecule sensing domain called an aptamer 
B)  There are known pathogens that use riboswitches as part of their mechanism 
C)  They respond to specific molecules and control translation in a way that is often similar to transcription attenuation 
D)  They contain a sensing domain and a decision making domain 
E)  All of these

Question 3

Which of the following is involved in regulation of eukaryotic transcription?

Accepted Answer

A)  Histone acetyltransferase 
B)  Histone deacetylase 
C)  Chromatin remodeling complexes 
D)  Octamer sliding 
E)  All of the choices 
A)  Histone acetyltransferase 
B)  Histone deacetylase 
C)  Chromatin remodeling complexes 
D)  Octamer sliding 
E)  All of the choices

Question 4

Control of transcription in prokaryotes does not involve

Accepted Answer

A)  enhancers. 
B)  silencers. 
C)  leucine zipper proteins. 
D)  alternative σ factors. 
A)  enhancers. 
B)  silencers. 
C)  leucine zipper proteins. 
D)  alternative σ factors.

Question 5

There is only one sigma subunit, since the same RNA Polymerase must bind to all genes in E. coli.

Accepted Answer

A) True 
 B)False

Question 6

The leucine zipper is different from other DNA binding domains, since it requires the DNA-binding protein to form a dimer.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following RNAs is noted for having a "cloverleaf" structure?

Accepted Answer

A)  mRNA 
B)  rRNA 
C)  tRNA 
D)  All of these 
A)  mRNA 
B)  rRNA 
C)  tRNA 
D)  All of these

Question 8

What provides the energy for the conversion from the open complex to chain elongation?

Accepted Answer

A)  ATP hydrolysis distinct from any incorporation into the chain. 
B)  Nucleotide hydrolysis associated with incorporation into the chain. 
C)  Torsional stress built into the separating DNA strands. 
D)  Binding of rho ( ρ ) factor to the holoenzyme. 
E)  None of these 
A)  ATP hydrolysis distinct from any incorporation into the chain. 
B)  Nucleotide hydrolysis associated with incorporation into the chain. 
C)  Torsional stress built into the separating DNA strands. 
D)  Binding of rho ( ρ ) factor to the holoenzyme. 
E)  None of these

Question 9

What is the function of the sigma ( σ ) subunit of RNA polymerase in E. coli ?

Accepted Answer

A)  It recognizes promoters where transcription should begin. 
B)  It contains the active site for synthesis of RNA. 
C)  It ensures proper processitvity of the polymerase, so it doesn't stop prematurely. 
D)  It is involved in chain termination. 
E)  It scrunches the DNA 
A)  It recognizes promoters where transcription should begin. 
B)  It contains the active site for synthesis of RNA. 
C)  It ensures proper processitvity of the polymerase, so it doesn't stop prematurely. 
D)  It is involved in chain termination. 
E)  It scrunches the DNA

Question 10

A transcription factor is

Accepted Answer

A)  a subunit of RNA polymerase II that does not have a prokaryotic analogue. 
B)  the part of the promoter sequence closest to the start of transcription. 
C)  a protein other than RNA polymerase that is involved in transcription. 
D)  a sequence that determines whether an upstream element will be an enhancer or silencer. 
A)  a subunit of RNA polymerase II that does not have a prokaryotic analogue. 
B)  the part of the promoter sequence closest to the start of transcription. 
C)  a protein other than RNA polymerase that is involved in transcription. 
D)  a sequence that determines whether an upstream element will be an enhancer or silencer.

Question 11

Which of the conditions would result in the greatest amount of transcription of the lac operon?

Accepted Answer

A)  I 
B)  II 
C)  III 
D)  IV 
A)  I 
B)  II 
C)  III 
D)  IV

Question 12

Post-transcription processing of mRNA in prokaryotes is required.

Accepted Answer

A) True 
 B)False

Question 13

RNA synthesis begins at the base in the DNA sequence designated by the following number:

Accepted Answer

A)  +1 (plus one) 
B)  0 (zero) 
C)  − 1 (minus one) 
D)  − 10 region (minus ten) 
E)  It varies between genes. 
A)  +1 (plus one) 
B)  0 (zero) 
C)  − 1 (minus one) 
D)  − 10 region (minus ten) 
E)  It varies between genes.

Question 14

The weakness of A − U base pairs at the end of the RNA molecule may help in dissociation of the new RNA product.

Accepted Answer

A) True 
 B)False

Question 15

​ In the template DNA strand formed during transcription, the area from the end of the Upstream Promoter (UP) element to the transcription start site (TSS) is called the _____.

Accepted Answer

A)  proximal promoter 
B)  core promoter 
C)  distal promoter 
D)  extended promoter 
A)  proximal promoter 
B)  core promoter 
C)  distal promoter 
D)  extended promoter

Question 16

Alternative removal of exons from mRNA is important in which of the following?

Accepted Answer

A)  Troponins and other contractile proteins. 
B)  Different types of collagen. 
C)  The disease lupus. 
D)  Alzheimer's disease. 
E)  All of these. 
A)  Troponins and other contractile proteins. 
B)  Different types of collagen. 
C)  The disease lupus. 
D)  Alzheimer's disease. 
E)  All of these.

Question 17

The sequences in eukaryotic DNA known as introns are

Accepted Answer

A)  those included in the final sequence of messenger RNA 
B)  the intervening sequences not expressed in the final sequence of messenger RNA 
C)  the binding sites for DNA polymerase 
D)  the binding sites for RNA polymerase 
A)  those included in the final sequence of messenger RNA 
B)  the intervening sequences not expressed in the final sequence of messenger RNA 
C)  the binding sites for DNA polymerase 
D)  the binding sites for RNA polymerase

Question 18

Which of the following is not a key step in the activation of mRNA synthesis in eukaryotes?

Accepted Answer

A)  Binding of TBP to the DNA. 
B)  Binding of other transcription factors. 
C)  Binding of RNA Pol I. 
D)  Phosphorylation of the RNA Pol. 
E)  All of these are necessary to initiate RNA synthesis in eukaryotes. 
A)  Binding of TBP to the DNA. 
B)  Binding of other transcription factors. 
C)  Binding of RNA Pol I. 
D)  Phosphorylation of the RNA Pol. 
E)  All of these are necessary to initiate RNA synthesis in eukaryotes.

Question 19

Which category of ribozymes requires an external guanosine frothier action?

Accepted Answer

A)  Group I 
B)  Group II 
C)  Both Group I & Group II 
D)  Neither Group I nor Group II 
A)  Group I 
B)  Group II 
C)  Both Group I & Group II 
D)  Neither Group I nor Group II

Question 20

Which of the following influence the amount of expression of an operon?

Accepted Answer

A)  Availability of the specific sigma factor for that operon. 
B)  How well the Pribnow box conforms to the consensus sequence. 
C)  Attenuation mechanisms. 
D)  Presence of 3' 5' cyclic AMP. 
E)  All of these. 
A)  Availability of the specific sigma factor for that operon. 
B)  How well the Pribnow box conforms to the consensus sequence. 
C)  Attenuation mechanisms. 
D)  Presence of 3' 5' cyclic AMP. 
E)  All of these.

Exam 11: Transcription of the Genetic Code the Biosynthesis of Rna

The eukaryotic TATA-binding protein (TBP) functions in a manner similar to

Which of the following is true about riboswitches:

Which of the following is involved in regulation of eukaryotic transcription?

Control of transcription in prokaryotes does not involve

There is only one sigma subunit, since the same RNA Polymerase must bind to all genes in E. coli.

The leucine zipper is different from other DNA binding domains, since it requires the DNA-binding protein to form a dimer.

Which of the following RNAs is noted for having a "cloverleaf" structure?

What provides the energy for the conversion from the open complex to chain elongation?

What is the function of the sigma ( σ ) subunit of RNA polymerase in E. coli ?

A transcription factor is

Which of the conditions would result in the greatest amount of transcription of the lac operon?

Post-transcription processing of mRNA in prokaryotes is required.

RNA synthesis begins at the base in the DNA sequence designated by the following number:

The weakness of A − U base pairs at the end of the RNA molecule may help in dissociation of the new RNA product.

​ In the template DNA strand formed during transcription, the area from the end of the Upstream Promoter (UP) element to the transcription start site (TSS) is called the _____.

Alternative removal of exons from mRNA is important in which of the following?

The sequences in eukaryotic DNA known as introns are

Which of the following is not a key step in the activation of mRNA synthesis in eukaryotes?

Which category of ribozymes requires an external guanosine frothier action?

Which of the following influence the amount of expression of an operon?

Biochemistry and the Organization of Cells

Water the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins Enzymes

The Behavior of Proteins Enzymes Mechanisms and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids How Structure Conveys Information

Biosynthesis of Nucleic Acids Replication

Protein Synthesis Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses Cancer and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism Cellular Signaling

Filters

In the template DNA strand formed during transcription, the area from the end of the Upstream Promoter (UP) element to the transcription start site (TSS) is called the _____.