Question 1

Which of the following technologies allows the identification of bacteria within minutes from a culture plate?

Accepted Answer

A) Electron microscopy. 
B) MALDI-TOF MS. 
C) India ink stain. 
D) Blood culture. 
E) Point-of-care testing. 
A) Electron microscopy. 
B) MALDI-TOF MS. 
C) India ink stain. 
D) Blood culture. 
E) Point-of-care testing.

Question 2

Culture of a clinical specimen in the microbiology laboratory

Accepted Answer

A) always provides an accurate diagnosis. 
B) is always the best method for diagnosing an infection. 
C) typically provides a result in less than four hours. 
D) is especially useful for viruses which can't be seen in a microscope. 
E) none of the above. 
A) always provides an accurate diagnosis. 
B) is always the best method for diagnosing an infection. 
C) typically provides a result in less than four hours. 
D) is especially useful for viruses which can't be seen in a microscope. 
E) none of the above.

Question 3

A provisional identification of the cause of an infection can sometimes be made by direct microscopic examination of a clinical specimen.Which of the following diseases could NOT be diagnosed by this means?

Accepted Answer

A) Meningococcal disease. 
B) Urinary tract infection. 
C) Tuberculosis. 
D) Malaria. 
E) Cryptococcal meningitis. 
A) Meningococcal disease. 
B) Urinary tract infection. 
C) Tuberculosis. 
D) Malaria. 
E) Cryptococcal meningitis.

Question 4

The results of bacterial culture and antibiotic sensitivity tests on most clinical specimens are usually available after

Accepted Answer

A) 2-4 hours. 
B) 6 hours. 
C) 8-12 hours. 
D) 36-48 hours. 
E) 1 week. 
A) 2-4 hours. 
B) 6 hours. 
C) 8-12 hours. 
D) 36-48 hours. 
E) 1 week.

Question 5

If a patient's antibody titre to hepatitis B has recently changed from less than 20 to 640,it can be assumed that the patient has

Accepted Answer

A) not got hepatitis B. 
B) seroconverted. 
C) convalesced. 
D) recovered. 
E) immune deficiency. 
A) not got hepatitis B. 
B) seroconverted. 
C) convalesced. 
D) recovered. 
E) immune deficiency.

Question 6

Which of the following is NOT important when collecting specimens for microbiological investigation?

Accepted Answer

A) Time of collection. 
B) Correct labelling of the container. 
C) Using a sterile container. 
D) Collecting duplicate samples. 
E) Using aseptic technique. 
A) Time of collection. 
B) Correct labelling of the container. 
C) Using a sterile container. 
D) Collecting duplicate samples. 
E) Using aseptic technique.

Question 7

A specimen collected from a patient for culture in the microbiology laboratory

Accepted Answer

A) should always be refrigerated prior to sending it to the laboratory. 
B) should always be shipped in transport medium. 
C) should be taken before antibiotic therapy is commenced wherever possible. 
D) is rarely infectious after it has been collected. 
E) all of the above. 
A) should always be refrigerated prior to sending it to the laboratory. 
B) should always be shipped in transport medium. 
C) should be taken before antibiotic therapy is commenced wherever possible. 
D) is rarely infectious after it has been collected. 
E) all of the above.

Question 8

Identification of slow-growing bacteria can be achieved faster by the use of

Accepted Answer

A) polymerase chain reaction (PCR). 
B) increased incubation temperature for growth. 
C) detection of serum antibodies. 
D) electron microscopy. 
E) the streak plate method. 
A) polymerase chain reaction (PCR). 
B) increased incubation temperature for growth. 
C) detection of serum antibodies. 
D) electron microscopy. 
E) the streak plate method.

Question 9

Anaerobic bacteria present in a purulent discharge should be protected from oxygen while being transported to the laboratory.This is done by

Accepted Answer

A) placing the specimen in ice. 
B) transporting the specimen in the collection syringe. 
C) using a sterile container. 
D) using a specialised transport medium. 
E) placing the specimen in a CO₂ chamber. 
A) placing the specimen in ice. 
B) transporting the specimen in the collection syringe. 
C) using a sterile container. 
D) using a specialised transport medium. 
E) placing the specimen in a CO₂ chamber.

Question 10

The growth of microorganisms on agar in a petri dish is called a

Accepted Answer

A) culture. 
B) medium. 
C) sample. 
D) specimen. 
E) broth. 
A) culture. 
B) medium. 
C) sample. 
D) specimen. 
E) broth.

Question 11

Blood cultures

Accepted Answer

A) may not yield a result for several days or more. 
B) should be collected from all patients on admission to hospital. 
C) refers to a special method in which swab specimens are cultured in blood. 
D) require the collection of 500 ml of blood from the patient. 
E) should not be collected more than once from a patient. 
A) may not yield a result for several days or more. 
B) should be collected from all patients on admission to hospital. 
C) refers to a special method in which swab specimens are cultured in blood. 
D) require the collection of 500 ml of blood from the patient. 
E) should not be collected more than once from a patient.

Question 12

Which of the following statements concerning the laboratory diagnosis of urinary tract infections is TRUE?

Accepted Answer

A) All urine samples contain some microorganisms because the bladder has a normal microbiota. 
B) A midstream specimen of urine (MSU)is usually the most appropriate specimen to collect. 
C) Urine is toxic for all microorganisms and thus should not be allowed to stand after collection. 
D) Any bacteria found in a urine specimen is indicative of infection. 
E) Contamination of a urine specimen is usually by microorganisms from the air. 
A) All urine samples contain some microorganisms because the bladder has a normal microbiota. 
B) A midstream specimen of urine (MSU)is usually the most appropriate specimen to collect. 
C) Urine is toxic for all microorganisms and thus should not be allowed to stand after collection. 
D) Any bacteria found in a urine specimen is indicative of infection. 
E) Contamination of a urine specimen is usually by microorganisms from the air.

Question 13

The laboratory technique in which a microorganism can be specifically identified by identifying a segment of its DNA is called

Accepted Answer

A) hybridisation. 
B) complement fixation. 
C) radioimmunoassay. 
D) a nucleic acid probe. 
E) molecular serology. 
A) hybridisation. 
B) complement fixation. 
C) radioimmunoassay. 
D) a nucleic acid probe. 
E) molecular serology.

Question 14

A transport medium for microbiological specimens is designed to

Accepted Answer

A) keep the causative organism growing and multiplying. 
B) prevent the specimen from drying out. 
C) inhibit any normal microbiota organisms in the specimen. 
D) enable the specimen to be frozen if necessary. 
E) all of the above. 
A) keep the causative organism growing and multiplying. 
B) prevent the specimen from drying out. 
C) inhibit any normal microbiota organisms in the specimen. 
D) enable the specimen to be frozen if necessary. 
E) all of the above.

Question 15

An antigen-antibody reaction that results in clumping of particles is called

Accepted Answer

A) precipitation. 
B) complement fixation. 
C) agglutination. 
D) enzyme linkage. 
E) seroconversion. 
A) precipitation. 
B) complement fixation. 
C) agglutination. 
D) enzyme linkage. 
E) seroconversion.

Which of the following technologies allows the identification of bacteria within minutes from a culture plate?

Culture of a clinical specimen in the microbiology laboratory

A provisional identification of the cause of an infection can sometimes be made by direct microscopic examination of a clinical specimen.Which of the following diseases could NOT be diagnosed by this means?

The results of bacterial culture and antibiotic sensitivity tests on most clinical specimens are usually available after

If a patient's antibody titre to hepatitis B has recently changed from less than 20 to 640,it can be assumed that the patient has

Which of the following is NOT important when collecting specimens for microbiological investigation?

A specimen collected from a patient for culture in the microbiology laboratory

Identification of slow-growing bacteria can be achieved faster by the use of

Anaerobic bacteria present in a purulent discharge should be protected from oxygen while being transported to the laboratory.This is done by

The growth of microorganisms on agar in a petri dish is called a

Blood cultures

Which of the following statements concerning the laboratory diagnosis of urinary tract infections is TRUE?

The laboratory technique in which a microorganism can be specifically identified by identifying a segment of its DNA is called

A transport medium for microbiological specimens is designed to

An antigen-antibody reaction that results in clumping of particles is called

The Invisible World

Biological Reactions in Microbial Cells

Bacteria

Genes and Biotechnology

Viruses and Viral Diseases

Eucaryotic Microorganisms: Fungi, protozoa and Multicellular Organisms

Hostmicrobe Interactions and Principles of Disease

Epidemiology: How Diseases Are Spread

The Bodys Defence Systems

Pathogenic Mechanisms and Evasion Strategies of Microorganisms

Principles of Sterilisation and Disinfection

Antimicrobial Therapy

Infection Control in Healthcare Facilities

Issues in Public Health

Skin, wound and Eye Infections

Respiratory Tract Infections

Gastrointestinal Tract Infections

Cardiovascular and Multisystem Infections

Infections of the Nervous System

Infections of the Urinary and Reproductive Systems

Filters

Exam 15: Microbial Techniques for Diagnosis of Infection

Which of the following technologies allows the identification of bacteria within minutes from a culture plate?

Culture of a clinical specimen in the microbiology laboratory

A provisional identification of the cause of an infection can sometimes be made by direct microscopic examination of a clinical specimen.Which of the following diseases could NOT be diagnosed by this means?

The results of bacterial culture and antibiotic sensitivity tests on most clinical specimens are usually available after

If a patient's antibody titre to hepatitis B has recently changed from less than 20 to 640,it can be assumed that the patient has

Which of the following is NOT important when collecting specimens for microbiological investigation?

A specimen collected from a patient for culture in the microbiology laboratory

Identification of slow-growing bacteria can be achieved faster by the use of

Anaerobic bacteria present in a purulent discharge should be protected from oxygen while being transported to the laboratory.This is done by

The growth of microorganisms on agar in a petri dish is called a

Blood cultures

Which of the following statements concerning the laboratory diagnosis of urinary tract infections is TRUE?

The laboratory technique in which a microorganism can be specifically identified by identifying a segment of its DNA is called

A transport medium for microbiological specimens is designed to

An antigen-antibody reaction that results in clumping of particles is called

The Invisible World

Biological Reactions in Microbial Cells

Bacteria

Genes and Biotechnology

Viruses and Viral Diseases

Eucaryotic Microorganisms: Fungi, protozoa and Multicellular Organisms

Hostmicrobe Interactions and Principles of Disease

Epidemiology: How Diseases Are Spread

The Bodys Defence Systems

Pathogenic Mechanisms and Evasion Strategies of Microorganisms

Principles of Sterilisation and Disinfection

Antimicrobial Therapy

Infection Control in Healthcare Facilities

Issues in Public Health

Skin, wound and Eye Infections

Respiratory Tract Infections

Gastrointestinal Tract Infections

Cardiovascular and Multisystem Infections

Infections of the Nervous System

Infections of the Urinary and Reproductive Systems

Filters