Exam 18: Recombinant Dna and Biotechnology

Which characteristic would not be useful in a vector?

Which DNA sequence forms a palindromic restriction site?

Which tool or technique is most likely to serve a purpose similar to that of gene guns?

Which characteristic makes plasmids useful as vectors?

What are small, circular DNA molecules that replicate autonomously in many prokaryotic cells?

Which enzymes used in biotechnology perform opposite functions?

Which is a pesticide that acts by inhibiting an enzyme system in the chloroplast?

An miRNA has the sequence 5′-GAUCGCAUUCAG-3′.Which motif on mRNA would most likely be a target of this miRNA?

Biologists examining social structure in baboons are interested in which genes in the genome change in expression when changes in the dominance hierarchy occur.One tool of biotechnology they can use to assess such changes is _______.Once candidate genes have been found, RT-PCR can be used for confirmation.

Which DNA sequence is most likely a palindromic restriction site? (Note: Only the 5ʹ-to-3ʹ strand is shown.)

Refer to the table showing results from an experiment in which researchers used CRISPR technology to knock out various genes from a plant.They then examined plant stem height (in centimeters, with calculated means) and seed production (number of seeds, with calculated means) with each treatment.(Note: The "x" marks genes that have been knocked out.) Some researchers have suggested that trade-offs exist between plant stem height and seed production-if too many resources are used in growing stems, seed production may suffer.Do the results in the table lend support to that hypothesis? Explain your answer.

Refer to the table showing results from an experiment in which researchers used CRISPR technology to knock out various genes from a fly.They then examined the developmental time (in days, with calculated means) and larval survival (percent, with calculated means) for each treatment.(Note: The "x" marks genes that have been knocked out.) Besides CRISPR, what two other technologies could be used for such studies examining effects of knocking out gene expression? Of these two, which is generally preferred, and why?

Biologists are using transgenics to produce large quantities of a human protein in yeast cells in liquid medium.They find the cells are producing satisfactory levels of the protein, but the protein is not going to the appropriate locations in the cells.The most likely defect in the expression vector used is in the _______.

Refer to the figure showing an experiment using selectable marker (reporter) genes. Which conclusion is supported by this experiment?

A vector designed for the expression of a eukaryotic transgene in E.coli requires a

Which enzyme could be used to join two DNA fragments?

What changes are typically made to the Ti plasmid when it is used to transform plant cells?

Which statement about genomic and cDNA libraries is true?

Suppose one restriction enzyme (enzyme A) makes two cuts in a circular DNA molecule, and another restriction enzyme (enzyme B) makes one cut in that same DNA.The cuts are not in the same place, and the cut fragments are not exactly the same size.The restriction digestion is set up on an electrophoresis gel.If both enzymes are used in the restriction digestion, the number of bands the gel should pick up is _______.

The lacZ gene encodes